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ALG11 (NM_001004127) Human Tagged ORF Clone
CAT＃: RG218890
- TrueORF®
ALG11 (tGFP-tagged) - Human asparagine-linked glycosylation 11, alpha-1,2-mannosyltransferase homolog (yeast) (ALG11), transcript variant A
ORF Plasmid: DDK
"NM_001004127" in other vectors (4)
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CNY 4,750.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CDG1P; GT8 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG218890 representing NM_001004127
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCGGCGAAAGGAGCTGGTGCCTGTGCAAGTTGTTGAGGTTTTTTTATTCATTATTCTTCCCTG GGCTCATTGTATGTGGAACTTTATGTGTGTGTTTGGTCATTGTCCTTTGGGGAATCAGACTGCTGCTACA GAGAAAGAAAAAATTAGTGTCAACTAGCAGAAATGGGAAAAATCAAATGGTGATTGCATTTTTTCATCCA TACTGCAATGCTGGTGGAGGAGGAGAAAGAGTTTTATGGTGTGCTTTAAGAGCCCTGCAGAAAAAGTATC CTGAAGCAGTTTATGTTGTTTATACCGGCGATGTTAATGTCAACGGTCAACAGATACTAGAAGGTGCTTT CAGAAGATTTAACATCAGATTAATTCACCCAGTGCAGTTTGTTTTTTTAAGGAAACGCTATCTTGTGGAA GATTCACTGTATCCTCACTTCACACTGCTGGGCCAAAGTCTAGGATCCATTTTTCTTGGCTGGGAAGCTC TAATGCAGTGTGTTCCTGATGTTTACATTGATTCAATGGGATACGCTTTTACGCTTCCTCTGTTTAAGTA TATAGGGGGTTGCCAAGTTGGAAGCTATGTTCATTATCCTACTATCAGCACCGACATGCTCTCTGTAGTG AAGAATCAAAATATTGGATTTAATAATGCAGCCTTCATTACCAGGAATCCTTTTCTCAGCAAAGTAAAGC TCATCTACTACTATTTATTTGCTTTTATTTATGGACTTGTTGGTTCTTGCAGTGATGTAGTCATGGTCAA TTCTTCTTGGACACTAAACCATATTCTCTCACTATGGAAAGTTGGGAATTGCACTAACATTGTTTATCCA CCTTGTGATGTGCAGACATTTCTGGACATTCCCTTACATGAGAAAAAGATGACCCCAGGACATTTGCTGG TTTCTGTTGGCCAGTTTAGGCCGGAAAAGAATCATCCATTGCAGATCAGAGCCTTTGCTAAATTGCTGAA TAAGAAGATGGTTGAGTCACCTCCTTCGCTTAAACTTGTCCTCATTGGAGGTTGTCGTAACAAAGATGAT GAACTTAGGGTAAACCAACTGAGAAGGCTGTCTGAGGATTTAGGAGTTCAAGAATATGTGGAATTTAAAA TAAACATTCCATTTGATGAATTAAAGAATTATTTGTCTGAAGCAACAATTGGTCTGCATACCATGTGGAA CGAGCATTTTGGGATTGGAGTTGTGGAGTGTATGGCAGCTGGCACAATTATCCTTGCACACAATTCGGGG GGCCCAAAGCTTGACATTGTGATTCCTCACGAAGGAGATATAACTGGCTTTCTGGCTGAGAGTGAAGAAG ACTATGCTGAAACTATCGCTCACATTCTTTCCATGTCTGCAGAAAAGAGACTCCAAATCAGAAAAAGTGC TCGTGCATCTGTAAGCAGATTCTCTGATCAGGAATTTGAAGTGACATTCCTATCATCTGTGGAAAAGTTA TTTAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218890 representing NM_001004127
Red=Cloning site Green=Tags(s) MAAGERSWCLCKLLRFFYSLFFPGLIVCGTLCVCLVIVLWGIRLLLQRKKKLVSTSRNGKNQMVIAFFHP YCNAGGGGERVLWCALRALQKKYPEAVYVVYTGDVNVNGQQILEGAFRRFNIRLIHPVQFVFLRKRYLVE DSLYPHFTLLGQSLGSIFLGWEALMQCVPDVYIDSMGYAFTLPLFKYIGGCQVGSYVHYPTISTDMLSVV KNQNIGFNNAAFITRNPFLSKVKLIYYYLFAFIYGLVGSCSDVVMVNSSWTLNHILSLWKVGNCTNIVYP PCDVQTFLDIPLHEKKMTPGHLLVSVGQFRPEKNHPLQIRAFAKLLNKKMVESPPSLKLVLIGGCRNKDD ELRVNQLRRLSEDLGVQEYVEFKINIPFDELKNYLSEATIGLHTMWNEHFGIGVVECMAAGTIILAHNSG GPKLDIVIPHEGDITGFLAESEEDYAETIAHILSMSAEKRLQIRKSARASVSRFSDQEFEVTFLSSVEKL FK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001004127 |
| ORF Size | 1476 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001004127.1, NP_001004127.1 |
| RefSeq Size | 2585 bp |
| RefSeq ORF | 1479 bp |
| Locus ID | 440138 |
| UniProt ID | Q2TAA5 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| Gene Summary | This gene encodes a GDP-Man:Man3GlcNAc2-PP-dolichol-alpha1,2-mannosyltransferase which is localized to the cytosolic side of the endoplasmic reticulum (ER) and catalyzes the transfer of the fourth and fifth mannose residue from GDP-mannose (GDP-Man) to Man3GlcNAc2-PP-dolichol and Man4GlcNAc2-PP-dolichol resulting in the production of Man5GlcNAc2-PP-dolichol. Mutations in this gene are associated with congenital disorder of glycosylation type Ip (CDGIP). This gene overlaps but is distinct from the UTP14, U3 small nucleolar ribonucleoprotein, homolog C (yeast) gene. A pseudogene of the GDP-Man:Man3GlcNAc2-PP-dolichol-alpha1,2-mannosyltransferase has been identified on chromosome 19. [provided by RefSeq, Aug 2010] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218890 | ALG11 (Myc-DDK-tagged)-Human asparagine-linked glycosylation 11, alpha-1,2-mannosyltransferase homolog (yeast) (ALG11), transcript variant A |
CNY 3,656.00 |
|
| RC218890L3 | Lenti ORF clone of Human asparagine-linked glycosylation 11, alpha-1,2-mannosyltransferase homolog (yeast) (ALG11), transcript variant A, Myc-DDK-tagged |
CNY 6,180.00 |
|
| RC218890L4 | Lenti ORF clone of Human asparagine-linked glycosylation 11, alpha-1,2-mannosyltransferase homolog (yeast) (ALG11), transcript variant A, mGFP tagged |
CNY 6,180.00 |
|
| SC300606 | ALG11 (untagged)-Human asparagine-linked glycosylation 11, alpha-1,2-mannosyltransferase homolog (yeast) (ALG11), transcript variant A |
CNY 7,890.00 |
