Olfactory receptor 10X1 (OR10X1) (NM_001004477) Human Tagged ORF Clone

CAT#: RG218457

  • TrueORF®

OR10X1 (tGFP-tagged) - Human olfactory receptor, family 10, subfamily X, member 1 (OR10X1)

ORF Plasmid: DDK tGFP



  "NM_001004477" in other vectors (4)

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CNY 4,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit polyclonal anti-OR10X1 antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms OR1-13; OR1-14; OR10X1P
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG218457 representing NM_001004477
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGTTGAATGTTTATTGTTGTTTCTTTCAAATTTCAGACATTCAAACGATGAAGATCAACCAGACAA
TCCTGAAGGAATTCATTCTTGTTGGCTTTTCTGTGTACCCACATGTACAGACATTTCTTTTTGTGGTCTT
CTTTTGTCTCTACCTTCTCACCCTTGCAGGTAATCTGATCATCATGGGTCTAACTTGGGTGGACAGGTCC
CTCCACACCCCTATGTATCTCTTCCTTAGTGCACTCTCCTTCTCTGAGACCTGCTATACGCTGACCATCG
TCCCCAAGATGCTGGAAGATCTACTGGCCAAGGACAGAAGCATTTCAGTCACAGGTTGTAGCTTACAGAT
GTGCTTCTTCTTGGGACTTGGTGGCACAAACTGTATCATTCTCACTTTGATGGGATATGACCGCTTCCTG
GCCATCTGTAACCCTCTAAGATATCCACTGCTTATGACCAACATTGTATGTGGACAACTTGTGGCCTCTG
CTTGCACTGCAGGCTTCTTTATCTCTCTTACAGAGACTGCACTGATATTCAGGGACTCTTTCTGCAGACC
CAACCTTGTCAAACACTTCTTCTGCCATATGCTGGCAGTTATTAGGCTGTCTTGTATAGACAGTAACCAC
ACAGAATTCATTATAACACTGATCTCAGTGTCTGGTTTGCTGGGTACCCTTCTGCTCATCATCCTGACTG
ATGTCTTCATTATTTCTACTGTCCTCAGGATCCCTTCAGCTGAGGGCAAGCAGAAGGCCTTCACCACCTG
TGCCTCCCACCTCACCGTGGTTATAATCCACTTTGGTTTTGCATCTATTGTTTATTTGAAGCCAGAAGCC
TCAGGAGATGACACACTCATAGCAGTCCCTTATACTGTCATTACCCCCTTCCTCAGCCCCATCATATTCA
GCCTGAGGAATAAGGACATGAAAAATGCTTTTAGAAGAATGATGGGAAACACAGTTGCCTTGAAAAAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG218457 representing NM_001004477
Red=Cloning site Green=Tags(s)

MVLNVYCCFFQISDIQTMKINQTILKEFILVGFSVYPHVQTFLFVVFFCLYLLTLAGNLIIMGLTWVDRS
LHTPMYLFLSALSFSETCYTLTIVPKMLEDLLAKDRSISVTGCSLQMCFFLGLGGTNCIILTLMGYDRFL
AICNPLRYPLLMTNIVCGQLVASACTAGFFISLTETALIFRDSFCRPNLVKHFFCHMLAVIRLSCIDSNH
TEFIITLISVSGLLGTLLLIILTDVFIISTVLRIPSAEGKQKAFTTCASHLTVVIIHFGFASIVYLKPEA
SGDDTLIAVPYTVITPFLSPIIFSLRNKDMKNAFRRMMGNTVALKK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001004477
ORF Size 978 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001004477.1, NP_001004477.1
RefSeq Size 981 bp
RefSeq ORF 981 bp
Locus ID 128367
UniProt ID Q8NGY0
Protein Families Transmembrane
Protein Pathways Olfactory transduction
Gene Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. This olfactory receptor gene is a segregating pseudogene, where some individuals have an allele that encodes a functional olfactory receptor, while other individuals have an allele encoding a protein that is predicted to be non-functional. [provided by RefSeq, Jun 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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