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GPLD1 (NM_001503) Human Tagged ORF Clone
CAT＃: RG217578
- TrueORF®
GPLD1 (tGFP-tagged) - Human glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant 1
ORF Plasmid: DDK
"NM_001503" in other vectors (3)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217578 representing NM_001503
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGTCGCCATGGCCCGGGCAACGACGGCGACAGGAAACAGGTTGTGGCCTGGCCTGCTGATCATGT TGGGTTCTCTCTGCCATAGAGGTTCACCGTGTGGCCTTTCAACACACGTAGAAATAGGACACAGAGCTCT GGAGTTTCTTCAGCTTCACAATGGGCGTGTTAACTACAGAGAGCTGTTACTAGAACACCAGGATGCGTAT CAGGCTGGAATCGTGTTTCCTGATTGTTTTTACCCTAGCATCTGCAAAGGAGGAAAATTCCATGATGTGT CTGAGAGCACTCACTGGACTCCGTTTCTTAATGCAAGCGTTCATTATATCCGAGAGAACTATCCCCTTCC CTGGGAGAAGGACACAGAGAAACTGGTAGCTTTCTTGTTTGGAATTACTTCTCACATGGCGGCAGATGTC AGCTGGCATAGTCTGGGCCTTGAACAAGGATTCCTTAGGACCATGGGAGCTATTGATTTTCACGGCTCCT ATTCAGAGGCTCATTCGGCTGGTGATTTTGGAGGAGATGTGTTGAGCCAGTTTGAATTTAATTTTAATTA CCTTGCACGACGCTGGTATGTGCCAGTCAAAGATCTACTGGGAATTTATGAGAAACTGTATGGTCGAAAA GTCATCACCGAAAATGTAATCGTTGATTGTTCACATATCCAGTTCTTAGAAATGTATGGTGAGATGCTAG CTGTTTCCAAGTTATATCCCACTTACTCTACAAAGTCCCCGTTTTTGGTGGAACAATTCCAAGAGTATTT TCTTGGAGGACTGGATGATATGGCATTTTGGTCCACTAATATTTACCATCTAACAAGCTTCATGTTGGAG AATGGGACCAGTGACTGCAACCTGCCTGAGAACCCTCTGTTCATTGCATGTGGCGGCCAGCAAAACCACA CCCAGGGCTCAAAAATGCAGAAAAATGATTTTCACAGAAATTTGACTACATCCCTAACTGAAAGTGTTGA CAGGAATATAAACTATACTGAAAGAGGAGTGTTCTTTAGTGTAAATTCCTGGACCCCGGATTCCATGTCC TTTATCTACAAGGCTTTGGAAAGGAACATAAGGACAATGTTCATAGGTGGCTCTCAGTTGTCACAAAAGC ACGTCTCCAGCCCCTTAGCATCTTACTTCTTGTCATTTCCTTATGCGAGGCTTGGCTGGGCAATGACCTC AGCTGACCTCAACCAGGATGGGCACGGTGACCTCGTGGTGGGCGCACCAGGCTACAGCCGCCCCGGCCAC ATCCACATCGGGCGCGTGTACCTCATCTACGGCAATGACCTGGGCCTGCCACCTGTTGACCTGGACCTGG ACAAGGAGGCCCACAGGATCCTTGAAGGCTTCCAGCCCTCAGGTCGGTTTGGCTCGGCCTTGGCTGTGTT GGACTTTAACGTGGACGGCGTGCCTGACCTGGCCGTGGGAGCTCCCTCGGTGGGCTCCGAGCAGCTCACC TACAAAGGTGCCGTGTATGTCTACTTTGGTTCCAAACAAGGAGGAATGTCTTCTTCCCCTAACATCACCA TTTCTTGCCAGGACATCTACTGTAACTTGGGCTGGACTCTCTTGGCTGCAGATGTGAATGGAGACAGTGA ACCCGATCTGGTCATCGGCTCCCCTTTTGCACCAGGTGGAGGGAAGCAGAAGGGAATTGTGGCTGCGTTT TATTCTGGCCCCAGCCTGAGCGACAAAGAAAAACTGAACGTGGAGGCAGCCAACTGGACGGTGAGAGGCG AGGAAGACTTCTCCTGGTTTGGATATTCCCTTCACGGTGTCACTGTGGACAACAGAACCTTGCTGTTGGT TGGGAGCCCGACCTGGAAGAATGCCAGCAGGCTGGGCCATTTGTTACACATCCGAGATGAGAAAAAGAGC CTTGGGAGGGTGTATGGCTACTTCCCACCAAACGGCCAAAGCTGGTTTACCATTTCTGGAGACAAGGCAA TGGGGAAACTGGGTACTTCCCTTTCCAGTGGCCACGTACTGATGAATGGGACTCTGAAACAAGTGCTGCT GGTTGGAGCCCCTACGTACGATGACGTGTCTAAGGTGGCATTCCTGACCGTGACCCTACACCAAGGCGGA GCCACTCGCATGTACGCACTCATATCTGACGCGCAGCCTCTGCTGCTCAGCACCTTCAGCGGAGACCGCC GCTTCTCCCGATTTGGTGGCGTTCTGCACTTGAGTGACCTGGATGATGATGGCTTAGATGAAATCATCAT GGCAGCCCCCCTGAGGATAGCAGATGTAACCTCTGGACTGATTGGGGGAGAAGACGGCCGAGTATATGTA TATAATGGCAAAGAGACCACCCTTGGTGACATGACTGGCAAATGCAAATCATGGATAACTCCATGTCCAG AAGAAAAGGCCCAATATGTATTGATTTCTCCTGAAGCCAGCTCAAGGTTTGGGAGCTCCCTCATCACCGT GAGGTCCAAGGCAAAGAACCAAGTCGTCATTGCTGCTGGAAGGAGTTCTTTGGGAGCCCGACTCTCCGGG GCACTTCACGTCTATAGCCTTGGCTCAGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217578 representing NM_001503
Red=Cloning site Green=Tags(s) MQVAMARATTATGNRLWPGLLIMLGSLCHRGSPCGLSTHVEIGHRALEFLQLHNGRVNYRELLLEHQDAY QAGIVFPDCFYPSICKGGKFHDVSESTHWTPFLNASVHYIRENYPLPWEKDTEKLVAFLFGITSHMAADV SWHSLGLEQGFLRTMGAIDFHGSYSEAHSAGDFGGDVLSQFEFNFNYLARRWYVPVKDLLGIYEKLYGRK VITENVIVDCSHIQFLEMYGEMLAVSKLYPTYSTKSPFLVEQFQEYFLGGLDDMAFWSTNIYHLTSFMLE NGTSDCNLPENPLFIACGGQQNHTQGSKMQKNDFHRNLTTSLTESVDRNINYTERGVFFSVNSWTPDSMS FIYKALERNIRTMFIGGSQLSQKHVSSPLASYFLSFPYARLGWAMTSADLNQDGHGDLVVGAPGYSRPGH IHIGRVYLIYGNDLGLPPVDLDLDKEAHRILEGFQPSGRFGSALAVLDFNVDGVPDLAVGAPSVGSEQLT YKGAVYVYFGSKQGGMSSSPNITISCQDIYCNLGWTLLAADVNGDSEPDLVIGSPFAPGGGKQKGIVAAF YSGPSLSDKEKLNVEAANWTVRGEEDFSWFGYSLHGVTVDNRTLLLVGSPTWKNASRLGHLLHIRDEKKS LGRVYGYFPPNGQSWFTISGDKAMGKLGTSLSSGHVLMNGTLKQVLLVGAPTYDDVSKVAFLTVTLHQGG ATRMYALISDAQPLLLSTFSGDRRFSRFGGVLHLSDLDDDGLDEIIMAAPLRIADVTSGLIGGEDGRVYV YNGKETTLGDMTGKCKSWITPCPEEKAQYVLISPEASSRFGSSLITVRSKAKNQVVIAAGRSSLGARLSG ALHVYSLGSD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001503 |
| ORF Size | 2520 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001503.2, NP_001494.2 |
| RefSeq Size | 3489 bp |
| RefSeq ORF | 2523 bp |
| Locus ID | 2822 |
| UniProt ID | P80108 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis |
| Gene Summary | Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217578 | GPLD1 (Myc-DDK-tagged)-Human glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant 1 |
CNY 6,152.00 |
|
| RC217578L3 | Lenti ORF clone of Human glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant 1, Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC217578L4 | Lenti ORF clone of Human glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant 1, mGFP tagged |
CNY 9,030.00 |
