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SI (NM_001041) Human Tagged ORF Clone
CAT＃: RG217558
- TrueORF®
SI (tGFP-tagged) - Human sucrase-isomaltase (alpha-glucosidase) (SI)
ORF Plasmid: DDK
"NM_001041" in other vectors (3)
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CNY 20,024.00
货期*
现货
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217558 representing NM_001041
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAGAAAGAAATTTAGTGGATTGGAAATCTCTCTGATTGTCCTTTTTGTCATAGTTACTATAATAG CTATTGCCTTAATTGTTGTTTTAGCAACTAAGACACCTGCTGTTGATGAAATTAGTGATTCTACTTCAAC TCCAGCTACTACTCGTGTGACTACAAATCCTTCTGATTCAGGAAAATGTCCAAATGTGTTAAATGATCCT GTCAATGTGAGAATAAACTGCATTCCAGAACAATTCCCAACAGAGGGAATTTGTGCACAGAGAGGCTGCT GCTGGAGGCCGTGGAATGACTCTCTTATTCCTTGGTGCTTCTTCGTTGATAATCATGGTTATAACGTTCA AGACATGACAACAACAAGTATTGGAGTTGAAGCCAAATTAAACAGGATACCTTCACCTACACTATTTGGA AATGACATCAACAGTGTTCTCTTCACAACTCAAAATCAGACACCCAATCGTTTCCGGTTCAAGATTACTG ATCCAAATAATAGAAGATATGAAGTTCCTCATCAGTATGTAAAAGAGTTTACTGGACCCACAGTTTCTGA TACGTTGTATGATGTGAAGGTTGCCCAAAACCCATTTAGCATCCAAGTTATTAGGAAAAGCAACGGTAAA ACTTTGTTTGACACCAGCATTGGTCCCTTAGTGTACTCTGACCAGTACTTACAGATCTCAGCCCGTCTTC CAAGTGATTATATTTATGGTATTGGAGAACAAGTTCATAAGAGATTTCGTCATGATTTATCCTGGAAAAC ATGGCCAATTTTTACTCGAGACCAACTTCCTGGTGATAATAATAATAATTTATACGGCCATCAAACATTC TTTATGTGTATTGAAGATACATCTGGAAAGTCATTCGGTGTTTTTTTAATGAATAGCAATGCAATGGAGA TTTTTATCCAGCCTACTCCAATAGTAACATATAGAGTTACCGGTGGCATTCTGGATTTTTACATCCTTCT AGGAGATACACCAGAACAAGTAGTTCAACAGTATCAACAGCTTGTTGGACTACCAGCAATGCCAGCATAT TGGAATCTTGGATTCCAACTAAGTCGCTGGAATTATAAGTCACTAGATGTAGTGAAAGAAGTGGTAAGGA GAAACCGGGAAGCTGGCATACCATTTGATACACAGGTCACTGATATTGACTACATGGAAGACAAGAAAGA CTTTACTTATGATCAAGTTGCGTTTAACGGACTCCCTCAATTTGTGCAAGATTTGCATGACCATGGACAG AAATATGTCATCATCTTGGACCCTGCAATTTCCATAGGTCGACGTGCCAATGGAACAACATATGCAACCT ATGAGAGGGGAAACACACAACATGTGTGGATAAATGAGTCAGATGGAAGTACACCAATTATTGGAGAGGT ATGGCCAGGATTAACAGTATACCCTGATTTCACTAATCCAAACTGCATTGATTGGTGGGCAAATGAATGC AGTATTTTCCATCAAGAAGTGCAATATGATGGACTTTGGATTGACATGAATGAAGTTTCCAGCTTTATTC AAGGTTCAACAAAAGGATGTAATGTAAACAAATTGAATTATCCACCGTTTACTCCTGATATTCTTGACAA ACTCATGTATTCCAAAACAATTTGCATGGATGCTGTGCAGAACTGGGGTAAACAGTATGATGTTCATAGC CTCTATGGATACAGCATGGCTATAGCCACAGAGCAAGCTGTACAAAAAGTTTTTCCTAATAAGAGAAGCT TCATTCTTACCCGCTCAACATTTGCTGGATCTGGAAGACATGCTGCTCATTGGTTAGGAGACAATACTGC TTCATGGGAACAAATGGAATGGTCTATAACTGGAATGCTGGAGTTCAGTTTGTTTGGAATACCTTTGGTT GGAGCAGACATCTGTGGATTTGTGGCTGAAACCACAGAAGAACTTTGCAGAAGATGGATGCAACTTGGGG CATTTTATCCATTTTCCAGAAACCATAATTCTGACGGATATGAACATCAGGATCCTGCATTTTTTGGGCA GAATTCACTTTTGGTTAAATCATCAAGGCAGTATTTAACTATTCGCTACACCTTATTACCCTTCCTCTAC ACTCTGTTTTATAAAGCCCATGTGTTTGGAGAAACAGTAGCAAGACCAGTTCTTCATGAGTTTTATGAGG ATACGAACAGCTGGATTGAGGACACTGAGTTTTTGTGGGGCCCTGCATTACTTATTACTCCTGTTCTAAA ACAGGGAGCAGATACTGTGAGTGCCTACATCCCTGATGCTATTTGGTATGATTATGAATCTGGTGCAAAA AGGCCATGGAGGAAACAACGGGTTGATATGTATCTTCCAGCAGACAAAATAGGATTACATCTTAGAGGAG GTTATATCATCCCCATTCAAGAACCAGATGTAACAACAACAGCAAGCCGTAAGAATCCTCTAGGACTTAT AGTCGCATTAGGTGAAAACAACACAGCCAAAGGAGACTTTTTCTGGGATGATGGAGAAACTAAAGATACA ATACAAAATGGCAACTACATATTATATACATTTTCAGTTTCTAATAACACATTAGATATTGTGTGCACAC ATTCATCATATCAGGAAGGAACTACCTTAGCATTTCAGACTGTAAAAATCCTTGGGTTGACAGACAGTGT TACAGAAGTTAGAGTGGCGGAAAATAATCAACCAATGAACGCTCATTCCAATTTCACTTATGATGCTTCT AACCAGGTTCTCCTAATTGCAGATCTCAAACTTAATCTTGGAAGAAACTTTAGTGTTCAATGGAATCAAA TTTTCTCAGAAAATGAAAGATTTAATTGTTATCCAGATGCAGATTTGGCAACTGAACAAAAGTGCACACA ACGTGGCTGTGTATGGAGAACGGGTTCTTCTCTATCCAAAGCACCTGAGTGTTACTTTCCCAGACAAGAT AACTCTTATTCAGTCAACTCAGCTCGCTATTCATCCATGGGTATAACAGCTGACCTCCAACTAAATACTG CAAATGCCAGAATAAAGTTACCTTCTGACCCCATCTCAACTCTTCGTGTGGAGGTGAAATATCACAAAAA TGATATGTTGCAGTTTAAGATTTATGATCCCCAAAAGAAGAGATATGAAGTACCAGTACCGTTAAACATT CCAACCACCCCAATAAGTACTTATGAAGACAGACTTTATGATGTGGAAATCAAGGAAAATCCTTTTGGCA TCCAGATTCGACGGAGAAGCAGTGGAAGAGTCATTTGGGATTCTTGGCTGCCTGGATTTGCTTTTAATGA CCAGTTCATTCAAATATCGACTCGCCTGCCATCAGAATATATATATGGTTTTGGGGAAGTGGAACATACA GCATTTAAGCGAGATCTGAACTGGAATACTTGGGGAATGTTCACAAGAGACCAACCCCCTGGTTACAAAC TTAATTCCTATGGATTTCATCCCTATTACATGGCTCTGGAAGAGGAGGGCAATGCTCATGGTGTTTTCTT ACTCAACAGCAATGCAATGGATGTTACATTCCAGCCAACTCCTGCTCTAACTTACCGTACAGTTGGAGGG ATCTTGGATTTTTATATGTTTTTGGGCCCAACTCCAGAAGTTGCAACAAAGCAATACCATGAAGTAATTG GCCATCCAGTCATGCCAGCTTATTGGGCTTTGGGATTCCAATTATGTCGTTATGGATATGCAAATACTTC AGAGGTTCGGGAATTATATGACGCTATGGTGGCTGCTAACATCCCCTATGATGTTCAGTACACAGACATT GACTACATGGAAAGGCAGCTAGACTTTACAATTGGTGAAGCATTCCAGGACCTTCCTCAGTTTGTTGACA AAATAAGAGGAGAAGGAATGAGATACATTATTATCCTGGATCCAGCAATTTCAGGAAATGAAACAAAGAC TTACCCTGCATTTGAAAGAGGACAGCAGAATGATGTCTTTGTCAAATGGCCAAACACCAATGACATTTGT TGGGCAAAGGTTTGGCCAGATTTGCCCAACATAACAATAGATAAAACTCTAACGGAAGATGAAGCTGTTA ATGCTTCCAGAGCTCATGTAGCTTTCCCAGATTTCTTCAGGACTTCCACAGCAGAGTGGTGGGCCAGAGA AATTGTGGACTTTTACAATGAAAAGATGAAGTTTGATGGTTTGTGGATTGATATGAATGAGCCATCAAGT TTTGTAAATGGAACAACTACTAATCAATGCAGAAATGACGAACTAAATTATCCACCTTATTTCCCAGAAC TCACAAAAAGAACTGATGGATTACATTTCAGAACAATTTGCATGGAAGCTGAGCAGATTCTTAGTGATGG AACATCAGTTTTGCATTACGATGTTCACAATCTCTATGGATGGTCACAGATGAAACCTACTCATGATGCA TTGCAGAAGACAACTGGAAAAAGAGGGATTGTAATTTCTCGTTCCACGTATCCTACTAGTGGACGATGGG GAGGACACTGGCTTGGAGACAACTATGCACGATGGGACAACATGGACAAATCAATCATTGGTATGATGGA ATTTAGTCTGTTTGGAATATCATATACTGGAGCAGACATCTGTGGTTTTTTCAACAACTCAGAATATCAT CTCTGTACCCGCTGGATGCAACTTGGAGCATTTTATCCATACTCAAGGAATCACAACATTGCAAATACTA GAAGACAAGATCCCGCTTCCTGGAATGAAACTTTTGCTGAAATGTCAAGGAATATTCTAAATATTAGATA CACCTTATTGCCCTATTTTTACACACAAATGCATGAAATTCATGCTAATGGTGGCACTGTTATCCGACCC CTTTTGCATGAGTTCTTTGATGAAAAACCAACCTGGGATATATTCAAGCAGTTCTTATGGGGTCCAGCAT TTATGGTTACCCCAGTACTGGAACCTTATGTTCAAACTGTAAATGCCTACGTCCCCAATGCTCGGTGGTT TGACTACCATACAGGCAAAGATATTGGCGTCAGAGGACAATTTCAAACATTTAATGCTTCTTATGACACA ATAAACCTACATGTCCGTGGTGGTCACATCCTACCATGTCAAGAGCCAGCTCAAAACACATTTTACAGTC GACAAAAACACATGAAGCTCATTGTTGCTGCAGATGATAATCAGATGGCACAGGGTTCTCTGTTTTGGGA TGATGGAGAGAGTATAGACACCTATGAAAGAGACCTATATTTATCTGTACAATTTAATTTAAACCAGACC ACCTTAACAAGCACTATATTGAAGAGAGGTTACATAAATAAAAGTGAAACGAGGCTTGGATCCCTTCATG TATGGGGGAAAGGAACTACTCCTGTCAATGCAGTTACTCTAACGTATAACGGAAATAAAAATTCGCTTCC TTTTAATGAAGACACTACCAACATGATATTACGTATTGATCTGACCACACACAATGTTACTCTAGAAGAA CCAATAGAAATCAACTGGTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217558 representing NM_001041
Red=Cloning site Green=Tags(s) MARKKFSGLEISLIVLFVIVTIIAIALIVVLATKTPAVDEISDSTSTPATTRVTTNPSDSGKCPNVLNDP VNVRINCIPEQFPTEGICAQRGCCWRPWNDSLIPWCFFVDNHGYNVQDMTTTSIGVEAKLNRIPSPTLFG NDINSVLFTTQNQTPNRFRFKITDPNNRRYEVPHQYVKEFTGPTVSDTLYDVKVAQNPFSIQVIRKSNGK TLFDTSIGPLVYSDQYLQISARLPSDYIYGIGEQVHKRFRHDLSWKTWPIFTRDQLPGDNNNNLYGHQTF FMCIEDTSGKSFGVFLMNSNAMEIFIQPTPIVTYRVTGGILDFYILLGDTPEQVVQQYQQLVGLPAMPAY WNLGFQLSRWNYKSLDVVKEVVRRNREAGIPFDTQVTDIDYMEDKKDFTYDQVAFNGLPQFVQDLHDHGQ KYVIILDPAISIGRRANGTTYATYERGNTQHVWINESDGSTPIIGEVWPGLTVYPDFTNPNCIDWWANEC SIFHQEVQYDGLWIDMNEVSSFIQGSTKGCNVNKLNYPPFTPDILDKLMYSKTICMDAVQNWGKQYDVHS LYGYSMAIATEQAVQKVFPNKRSFILTRSTFAGSGRHAAHWLGDNTASWEQMEWSITGMLEFSLFGIPLV GADICGFVAETTEELCRRWMQLGAFYPFSRNHNSDGYEHQDPAFFGQNSLLVKSSRQYLTIRYTLLPFLY TLFYKAHVFGETVARPVLHEFYEDTNSWIEDTEFLWGPALLITPVLKQGADTVSAYIPDAIWYDYESGAK RPWRKQRVDMYLPADKIGLHLRGGYIIPIQEPDVTTTASRKNPLGLIVALGENNTAKGDFFWDDGETKDT IQNGNYILYTFSVSNNTLDIVCTHSSYQEGTTLAFQTVKILGLTDSVTEVRVAENNQPMNAHSNFTYDAS NQVLLIADLKLNLGRNFSVQWNQIFSENERFNCYPDADLATEQKCTQRGCVWRTGSSLSKAPECYFPRQD NSYSVNSARYSSMGITADLQLNTANARIKLPSDPISTLRVEVKYHKNDMLQFKIYDPQKKRYEVPVPLNI PTTPISTYEDRLYDVEIKENPFGIQIRRRSSGRVIWDSWLPGFAFNDQFIQISTRLPSEYIYGFGEVEHT AFKRDLNWNTWGMFTRDQPPGYKLNSYGFHPYYMALEEEGNAHGVFLLNSNAMDVTFQPTPALTYRTVGG ILDFYMFLGPTPEVATKQYHEVIGHPVMPAYWALGFQLCRYGYANTSEVRELYDAMVAANIPYDVQYTDI DYMERQLDFTIGEAFQDLPQFVDKIRGEGMRYIIILDPAISGNETKTYPAFERGQQNDVFVKWPNTNDIC WAKVWPDLPNITIDKTLTEDEAVNASRAHVAFPDFFRTSTAEWWAREIVDFYNEKMKFDGLWIDMNEPSS FVNGTTTNQCRNDELNYPPYFPELTKRTDGLHFRTICMEAEQILSDGTSVLHYDVHNLYGWSQMKPTHDA LQKTTGKRGIVISRSTYPTSGRWGGHWLGDNYARWDNMDKSIIGMMEFSLFGISYTGADICGFFNNSEYH LCTRWMQLGAFYPYSRNHNIANTRRQDPASWNETFAEMSRNILNIRYTLLPYFYTQMHEIHANGGTVIRP LLHEFFDEKPTWDIFKQFLWGPAFMVTPVLEPYVQTVNAYVPNARWFDYHTGKDIGVRGQFQTFNASYDT INLHVRGGHILPCQEPAQNTFYSRQKHMKLIVAADDNQMAQGSLFWDDGESIDTYERDLYLSVQFNLNQT TLTSTILKRGYINKSETRLGSLHVWGKGTTPVNAVTLTYNGNKNSLPFNEDTTNMILRIDLTTHNVTLEE PIEINWS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001041 |
| ORF Size | 5481 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001041.1, NP_001032.1 |
| RefSeq Size | 6021 bp |
| RefSeq ORF | 5484 bp |
| Locus ID | 6476 |
| UniProt ID | P14410 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Metabolic pathways, Starch and sucrose metabolism |
| Gene Summary | This gene encodes a sucrase-isomaltase enzyme that is expressed in the intestinal brush border. The encoded protein is synthesized as a precursor protein that is cleaved by pancreatic proteases into two enzymatic subunits sucrase and isomaltase. These two subunits heterodimerize to form the sucrose-isomaltase complex. This complex is essential for the digestion of dietary carbohydrates including starch, sucrose and isomaltose. Mutations in this gene are the cause of congenital sucrase-isomaltase deficiency.[provided by RefSeq, Apr 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217558 | SI (Myc-DDK-tagged)-Human sucrase-isomaltase (alpha-glucosidase) (SI) |
CNY 18,424.00 |
|
| RC217558L1 | Lenti ORF clone of Human sucrase-isomaltase (alpha-glucosidase) (SI), Myc-DDK-tagged |
CNY 20,824.00 |
|
| SC302959 | SI (untagged)-Human sucrase-isomaltase (alpha-glucosidase) (SI) |
CNY 12,288.00 |
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