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IDI1 (NM_004508) Human Tagged ORF Clone
CAT＃: RG214341
- TrueORF®
IDI1 (tGFP-tagged) - Human isopentenyl-diphosphate delta isomerase 1 (IDI1)
ORF Plasmid: DDK
"NM_004508" in other vectors (7)
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CNY 4,370.00
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | IPP1; IPPI1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214341 representing NM_004508
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCGTGGACTGGCGCTGGCGCGAGCGATTGGCTGCGCGGCCCGGGGGCGGGGCCAGTGGGCGGTGC GCGCCGCAGACTGTGCTCAAAGCGGGCGCCATCCGGGACCGGCGGTTGTCTGTGGCCGGAGGCTGATCAG TGTTCTAGAACAGATCAGACATTTTGTAATGATGCCTGAAATAAACACTAACCACCTCGACAAGCAACAG GTTCAACTCCTGGCAGAGATGTGTATCCTTATTGATGAAAATGACAATAAAATTGGAGCTGAGACCAAGA AGAATTGTCACCTGAACGAGAACATTGAGAAAGGATTATTGCATCGAGCTTTTAGTGTCTTCTTATTCAA CACCGAAAATAAGCTTCTGCTACAGCAAAGATCAGATGCTAAGATTACCTTTCCAGGTTGTTTTACGAAT ACGTGTTGTAGTCATCCATTAAGCAATCCAGCCGAGCTTGAGGAAAGTGACGCCCTTGGAGTGAGGCGAG CAGCACAGAGACGGCTGAAAGCTGAGCTAGGAATTCCCTTGGAAGAGGTTCCTCCAGAAGAAATTAATTA TTTAACACGAATTCACTACAAAGCTCAGTCTGATGGTATCTGGGGTGAACATGAAATTGATTACATTTTG TTGGTGAGGAAGAATGTAACTTTGAATCCAGATCCCAATGAGATTAAAAGCTATTGTTATGTGTCAAAGG AAGAACTAAAAGAACTTCTGAAAAAAGCAGCCAGTGGTGAAATTAAGATAACGCCATGGTTTAAAATTAT TGCAGCGACTTTTCTCTTTAAATGGTGGGATAACTTAAATCATTTGAATCAGTTTGTTGACCATGAGAAA ATATACAGAATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214341 representing NM_004508
Red=Cloning site Green=Tags(s) MWRGLALARAIGCAARGRGQWAVRAADCAQSGRHPGPAVVCGRRLISVLEQIRHFVMMPEINTNHLDKQQ VQLLAEMCILIDENDNKIGAETKKNCHLNENIEKGLLHRAFSVFLFNTENKLLLQQRSDAKITFPGCFTN TCCSHPLSNPAELEESDALGVRRAAQRRLKAELGIPLEEVPPEEINYLTRIHYKAQSDGIWGEHEIDYIL LVRKNVTLNPDPNEIKSYCYVSKEELKELLKKAASGEIKITPWFKIIAATFLFKWWDNLNHLNQFVDHEK IYRM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004508 |
| ORF Size | 852 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004508.3, NP_004499.2 |
| RefSeq Size | 2150 bp |
| RefSeq ORF | 855 bp |
| Locus ID | 3422 |
| UniProt ID | Q13907 |
| Domains | NUDIX |
| Protein Pathways | Metabolic pathways, Terpenoid backbone biosynthesis |
| Gene Summary | IDI1 encodes a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP), which are the substrates for the successive reaction that results in the synthesis of farnesyl diphosphate and, ultimately, cholesterol. It has been shown in peroxisomal deficiency diseases such as Zellweger syndrome and neonatal adrenoleukodystrophy that there is reduction in IPP isomerase activity. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214341 | IDI1 (Myc-DDK-tagged)-Human isopentenyl-diphosphate delta isomerase 1 (IDI1) |
CNY 2,400.00 |
|
| RC214341L1 | Lenti ORF clone of Human isopentenyl-diphosphate delta isomerase 1 (IDI1), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC214341L2 | Lenti ORF clone of Human isopentenyl-diphosphate delta isomerase 1 (IDI1), mGFP tagged |
CNY 5,890.00 |
|
| RC214341L3 | Lenti ORF clone of Human isopentenyl-diphosphate delta isomerase 1 (IDI1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC214341L4 | Lenti ORF clone of Human isopentenyl-diphosphate delta isomerase 1 (IDI1), mGFP tagged |
CNY 5,890.00 |
|
| SC117309 | IDI1 (untagged)-Human isopentenyl-diphosphate delta isomerase 1 (IDI1) |
CNY 2,400.00 |
|
| SC317411 | IDI1 (untagged)-Human isopentenyl-diphosphate delta isomerase 1 (IDI1) |
CNY 6,270.00 |
