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Collagen IV alpha 6 (COL4A6) (NM_033641) Human Tagged ORF Clone
CAT＃: RG213974
- TrueORF®
COL4A6 (tGFP-tagged) - Human collagen, type IV, alpha 6 (COL4A6), transcript variant B
ORF Plasmid: DDK
"NM_033641" in other vectors (2)
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CNY 18,530.00
货期*
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CXDELq22.3; DELXq22.3; DFNX6 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG213974 representing NM_033641
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACCCTGGGTTGTGGCTGCTCCTGGTTACGTTGTGCCTGACCGAGGAACTGGCAGCAGCGGGAGAGA AGTCTTATGGAAAGCCATGTGGGGGCCAGGACTGCAGTGGGAGCTGTCAGTGTTTTCCTGAGAAAGGAGC GAGAGGACGACCTGGACCAATTGGAATTCAAGGCCCAACAGGTCCTCAAGGATTCACTGGCTCTACTGGT TTATCGGGATTGAAAGGAGAAAGGGGTTTCCCAGGCCTTCTGGGACCTTATGGACCAAAAGGAGATAAGG GTCCCATGGGAGTTCCTGGCTTTCTTGGCATCAATGGGATTCCGGGCCACCCTGGACAACCAGGCCCCAG AGGCCCACCTGGTCTGGATGGCTGTAATGGAACTCAAGGAGCTGTTGGATTTCCAGGCCCTGATGGCTAT CCTGGGCTTCTCGGACCACCCGGGCTTCCTGGTCAGAAAGGATCAAAAGGTGACCCTGTCCTTGCTCCAG GTAGTTTCAAAGGAATGAAGGGGGATCCTGGGCTGCCTGGACTGGATGGAATCACTGGCCCACAAGGAGC ACCCGGATTTCCTGGAGCTGTAGGACCTGCAGGACCACCAGGATTACAAGGTCCTCCAGGGCCTCCTGGT CCTCTTGGTCCTGATGGGAATATGGGGCTAGGTTTTCAAGGAGAGAAAGGAGTCAAGGGGGATGTTGGCC TCCCTGGCCCAGCAGGACCTCCACCATCTACTGGAGAGCTGGAATTCATGGGATTCCCCAAAGGGAAGAA AGGATCCAAGGGTGAACCAGGGCCTAAGGGTTTTCCAGGCATAAGTGGCCCTCCAGGCTTCCCGGGCCTT GGAACTACTGGAGAAAAGGGAGAAAAGGGAGAAAAGGGAATCCCTGGTTTGCCAGGACCTAGGGGTCCCA TGGGTTCAGAAGGAGTCCAAGGCCCTCCAGGGCAACAGGGCAAGAAAGGGACCCTGGGATTTCCTGGGCT TAATGGATTCCAAGGAATTGAGGGTCAAAAGGGTGACATTGGCCTGCCAGGCCCAGATGTTTTCATCGAT ATAGATGGTGCTGTGATCTCAGGTAATCCTGGAGATCCTGGTGTACCTGGCCTCCCAGGCCTTAAAGGAG ATGAAGGCATCCAAGGCCTACGTGGCCCTTCTGGTGTCCCTGGATTGCCAGCATTATCAGGTGTCCCAGG AGCCCTAGGGCCTCAGGGATTTCCAGGGCTGAAGGGGGACCAAGGAAACCCAGGCCGTACCACAATTGGA GCAGCTGGCCTCCCTGGCAGAGATGGTTTGCCAGGCCCACCAGGTCCACCAGGCCCACCTAGTCCAGAAT TTGAGACTGAAACTCTACACAACAAAGAGTCAGGGTTCCCTGGTCTCCGAGGAGAACAAGGTCCAAAAGG AAACCTAGGCCTCAAAGGAATAAAAGGAGACTCAGGTTTCTGTGCTTGTGACGGTGGTGTTCCCAACACT GGACCACCCGGGGAACCAGGCCCACCTGGTCCATGGGGTCTCATAGGCCTTCCAGGCCTTAAAGGAGCCA GAGGAGATCGAGGCTCTGGGGGTGCACAGGGCCCAGCAGGGGCTCCAGGCTTAGTTGGGCCTCTGGGTCC TTCAGGACCCAAAGGAAAGAAGGGGGAACCAATTCTCAGTACAATCCAAGGAATGCCAGGAGATCGGGGT GATTCTGGCTCCCAGGGCTTCCGTGGTGTAATAGGAGAACCAGGCAAGGACGGAGTACCAGGTTTACCAG GTCTGCCAGGCCTTCCGGGTGATGGTGGACAGGGCTTCCCAGGTGAAAAGGGGTTACCTGGACTTCCTGG TGAAAAAGGCCATCCTGGTCCACCTGGCCTCCCAGGAAATGGGTTACCAGGACTTCCTGGACCCCGTGGG CTTCCTGGAGATAAAGGCAAGGATGGATTACCGGGACAACAAGGCCTTCCCGGATCTAAGGGAATCACCC TGCCCTGTATTATTCCTGGGTCATACGGTCCATCAGGATTTCCAGGCACTCCCGGATTCCCAGGCCCTAA AGGGTCTCGAGGCCTCCCTGGGACCCCAGGCCAGCCTGGGTCAAGTGGAAGTAAAGGAGAGCCAGGGAGT CCAGGATTGGTTCATCTTCCTGAATTACCAGGATTTCCTGGACCTCGTGGGGAGAAGGGCTTGCCTGGGT TTCCTGGGCTCCCTGGAAAAGATGGCTTGCCTGGGATGATTGGCAGTCCAGGCTTACCTGGTTCCAAGGG AGCCACTGGTGACATCTTTGGTGCTGAAAATGGTGCTCCGGGGGAACAAGGCCTACAAGGATTAACAGGG CACAAAGGATTTCTTGGAGACTCTGGCCTTCCAGGACTCAAGGGTGTGCACGGGAAGCCTGGCTTACTAG GCCCCAAAGGTGAGCGGGGCAGCCCTGGGACACCAGGACAGGTGGGACAGCCAGGCACCCCAGGATCTAG TGGTCCATATGGCATCAAGGGCAAATCTGGGCTCCCAGGAGCACCAGGCTTCCCAGGCATCTCAGGACAT CCTGGAAAGAAAGGAACAAGAGGCAAGAAAGGTCCTCCTGGATCAATTGTAAAGAAAGGGCTGCCAGGGC TAAAAGGCCTTCCTGGAAATCCAGGCCTAGTAGGACTGAAAGGAAGCCCAGGCTCTCCAGGGGTCGCTGG GTTGCCAGCCCTCTCTGGACCCAAGGGAGAGAAGGGGTCTGTTGGATTCGTAGGTTTTCCAGGAATACCA GGTCTGCCTGGTATTCCTGGAACAAGAGGATTAAAAGGAATTCCAGGATCAACTGGAAAAATGGGACCAT CTGGACGTGCTGGTACTCCTGGTGAAAAGGGAGACAGAGGCAATCCGGGGCCAGTCGGAATACCTAGTCC AAGACGTCCAATGTCAAACCTTTGGCTCAAAGGAGACAAAGGCTCTCAAGGCTCAGCCGGATCCAATGGA TTTCCTGGGCCAAGAGGTGACAAAGGAGAGGCTGGTCGACCTGGACCACCAGGCCTACCTGGAGCTCCTG GCCTCCCAGGCATTATCAAAGGAGTTAGTGGAAAGCCAGGGCCCCCTGGCTTCATGGGAATCCGGGGCTT ACCTGGCCTGAAGGGGTCCTCTGGGATCACAGGTTTCCCAGGAATGCCAGGAGAAAGTGGTTCACAAGGT ATCAGAGGGTCGCCTGGACTCCCAGGAGCATCTGGTCTCCCAGGCCTGAAAGGAGACAACGGCCAGACAG TTGAAATTTCCGGTAGCCCAGGACCCAAGGGACAGCCTGGCGAATCTGGTTTTAAAGGCACAAAAGGAAG AGATGGACTAATAGGCAATATAGGCTTCCCTGGAAACAAAGGTGAAGATGGAAAAGTTGGTGTTTCTGGA GATGTTGGCCTTCCTGGAGCTCCAGGATTTCCAGGAGTTGCCGGCATGAGAGGAGAACCAGGACTTCCAG GTTCTTCTGGTCACCAAGGGGCAATTGGGCCTCTAGGATCCCCCGGATTAATAGGACCCAAAGGCTTCCC TGGATTTCCTGGTTTACATGGACTGAATGGGCTTCCGGGCACCAAGGGTACCCATGGCACTCCAGGACCT AGTATCACCGGTGTGCCTGGGCCTGCTGGTCTCCCTGGACCCAAAGGAGAAAAAGGATATCCAGGAATTG GCATCGGAGCTCCAGGGAAGCCGGGCCTGAGAGGGCAAAAAGGTGATCGAGGTTTCCCAGGTCTCCAGGG CCCTGCTGGTCTCCCCGGTGCCCCAGGCATCTCCTTGCCCTCACTCATAGCAGGACAGCCTGGTGACCCC GGGCGACCAGGCCTAGATGGAGAACGAGGCCGCCCAGGCCCCGCTGGACCCCCAGGTCCCCCTGGGCCAT CCTCGAATCAAGGCGACACCGGAGACCCTGGCTTCCCTGGAATTCCTGGACCTAAAGGGCCTAAGGGAGA CCAAGGAATTCCAGGTTTTTCTGGCCTCCCTGGAGAGCTAGGACTGAAAGGCATGAGAGGTGAGCCTGGC TTCATGGGGACTCCAGGCAAGGTTGGGCCACCTGGAGACCCAGGATTTCCCGGAATGAAGGGGAAGGCAG GGCCAAGAGGCTCTTCTGGCCTCCAAGGTGATCCTGGACAAACACCAACTGCAGAAGCTGTCCAGGTTCC TCCTGGACCCTTGGGTCTACCAGGGATCGATGGCATCCCTGGCCTCACTGGGGACCCTGGGGCTCAAGGC CCTGTAGGCCTACAAGGCTCCAAAGGTTTACCTGGCATCCCCGGTAAAGATGGCCCCAGTGGGCTCCCAG GCCCACCTGGGGCTCTTGGTGATCCTGGTCTGCCTGGACTGCAAGGCCCTCCAGGATTTGAAGGAGCTCC AGGGCAGCAAGGCCCCTTCGGGATGCCTGGAATGCCTGGCCAGAGCATGAGAGTGGGCTACACGTTGGTA AAGCACAGCCAGTCGGAACAGGTGCCCCCGTGTCCCATCGGGATGAGCCAGCTGTGGGTGGGGTACAGCT TACTGTTTGTGGAGGGGCAAGAGAAAGCCCACAACCAGGACCTGGGCTTTGCTGGCTCCTGTCTGCCCCG CTTCAGCACCATGCCCTTCATCTACTGCAACATCAACGAGGTGTGCCACTATGCCAGGCGCAATGATAAA TCTTACTGGCTCTCCACTACCGCCCCTATCCCCATGATGCCCGTCAGCCAGACCCAGATTCCCCAGTACA TCAGCCGCTGCTCTGTGTGTGAGGCACCCTCGCAAGCCATTGCTGTGCACAGCCAGGACATCACCATCCC GCAGTGCCCCCTGGGCTGGCGCAGCCTCTGGATTGGGTACTCTTTCCTCATGCACACTGCCGCTGGTGCC GAGGGTGGAGGCCAGTCCCTGGTCTCACCTGGCTCCTGCCTAGAGGACTTTCGGGCCACTCCTTTCATCG AATGCAGTGGTGCCCGAGGCACCTGCCACTACTTTGCAAACAAGTACAGTTTCTGGTTGACCACAGTGGA GGAGAGGCAGCAGTTTGGGGAGTTGCCTGTGTCTGAAACGCTGAAAGCTGGGCAGCTCCACACTCGAGTC AGTCGCTGCCAGGTGTGTATGAAAAGCCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213974 representing NM_033641
Red=Cloning site Green=Tags(s) MHPGLWLLLVTLCLTEELAAAGEKSYGKPCGGQDCSGSCQCFPEKGARGRPGPIGIQGPTGPQGFTGSTG LSGLKGERGFPGLLGPYGPKGDKGPMGVPGFLGINGIPGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGY PGLLGPPGLPGQKGSKGDPVLAPGSFKGMKGDPGLPGLDGITGPQGAPGFPGAVGPAGPPGLQGPPGPPG PLGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPKGFPGISGPPGFPGL GTTGEKGEKGEKGIPGLPGPRGPMGSEGVQGPPGQQGKKGTLGFPGLNGFQGIEGQKGDIGLPGPDVFID IDGAVISGNPGDPGVPGLPGLKGDEGIQGLRGPSGVPGLPALSGVPGALGPQGFPGLKGDQGNPGRTTIG AAGLPGRDGLPGPPGPPGPPSPEFETETLHNKESGFPGLRGEQGPKGNLGLKGIKGDSGFCACDGGVPNT GPPGEPGPPGPWGLIGLPGLKGARGDRGSGGAQGPAGAPGLVGPLGPSGPKGKKGEPILSTIQGMPGDRG DSGSQGFRGVIGEPGKDGVPGLPGLPGLPGDGGQGFPGEKGLPGLPGEKGHPGPPGLPGNGLPGLPGPRG LPGDKGKDGLPGQQGLPGSKGITLPCIIPGSYGPSGFPGTPGFPGPKGSRGLPGTPGQPGSSGSKGEPGS PGLVHLPELPGFPGPRGEKGLPGFPGLPGKDGLPGMIGSPGLPGSKGATGDIFGAENGAPGEQGLQGLTG HKGFLGDSGLPGLKGVHGKPGLLGPKGERGSPGTPGQVGQPGTPGSSGPYGIKGKSGLPGAPGFPGISGH PGKKGTRGKKGPPGSIVKKGLPGLKGLPGNPGLVGLKGSPGSPGVAGLPALSGPKGEKGSVGFVGFPGIP GLPGIPGTRGLKGIPGSTGKMGPSGRAGTPGEKGDRGNPGPVGIPSPRRPMSNLWLKGDKGSQGSAGSNG FPGPRGDKGEAGRPGPPGLPGAPGLPGIIKGVSGKPGPPGFMGIRGLPGLKGSSGITGFPGMPGESGSQG IRGSPGLPGASGLPGLKGDNGQTVEISGSPGPKGQPGESGFKGTKGRDGLIGNIGFPGNKGEDGKVGVSG DVGLPGAPGFPGVAGMRGEPGLPGSSGHQGAIGPLGSPGLIGPKGFPGFPGLHGLNGLPGTKGTHGTPGP SITGVPGPAGLPGPKGEKGYPGIGIGAPGKPGLRGQKGDRGFPGLQGPAGLPGAPGISLPSLIAGQPGDP GRPGLDGERGRPGPAGPPGPPGPSSNQGDTGDPGFPGIPGPKGPKGDQGIPGFSGLPGELGLKGMRGEPG FMGTPGKVGPPGDPGFPGMKGKAGPRGSSGLQGDPGQTPTAEAVQVPPGPLGLPGIDGIPGLTGDPGAQG PVGLQGSKGLPGIPGKDGPSGLPGPPGALGDPGLPGLQGPPGFEGAPGQQGPFGMPGMPGQSMRVGYTLV KHSQSEQVPPCPIGMSQLWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDK SYWLSTTAPIPMMPVSQTQIPQYISRCSVCEAPSQAIAVHSQDITIPQCPLGWRSLWIGYSFLMHTAAGA EGGGQSLVSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERQQFGELPVSETLKAGQLHTRV SRCQVCMKSL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033641 |
| ORF Size | 5070 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033641.4 |
| RefSeq Size | 6721 bp |
| RefSeq ORF | 5073 bp |
| Locus ID | 1288 |
| UniProt ID | Q14031 |
| Domains | C4, Collagen |
| Protein Pathways | ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer |
| Gene Summary | This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene, alpha 5 type IV collagen, so that the gene pair shares a common promoter. Deletions in the alpha 5 gene that extend into the alpha 6 gene result in diffuse leiomyomatosis accompanying the X-linked Alport syndrome caused by the deletion in the alpha 5 gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013] |
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