GFRAL (NM_207410) Human Tagged ORF Clone

CAT#: RG213001

  • TrueORF®

GFRAL (tGFP-tagged) - Human GDNF family receptor alpha like (GFRAL)

ORF Plasmid: DDK tGFP



  "NM_207410" in other vectors (6)

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CNY 7,088.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-GFRAL Antibody
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms bA360D14.1; C6orf144; GRAL; UNQ9356
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG213001 representing NM_207410
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATAGTGTTTATTTTCTTGGCTATGGGGTTAAGCTTGGAAAATGAATACACTTCCCAAACCAATAATT
GCACATATTTAAGAGAGCAATGCTTACGTGATGCAAATGGATGTAAACATGCTTGGAGAGTAATGGAAGA
TGCCTGCAATGATTCAGATCCAGGTGACCCCTGCAAGATGAGGAATTCATCATACTGTAACCTGAGTATC
CAGTACTTAGTGGAAAGCAATTTCCAATTTAAAGAGTGTCTTTGCACTGATGACTTCTATTGTACTGTGA
ACAAACTGCTTGGAAAAAAATGTATCAATAAATCAGATAACGTGAAAGAGGATAAATTCAAATGGAATCT
AACTACACGTTCCCATCATGGATTCAAAGGGATGTGGTCCTGTTTGGAAGTGGCAGAGGCATGTGTAGGG
GATGTGGTCTGTAATGCACAGTTGGCCTCTTACCTTAAAGCTTGCTCAGCAAATGGAAATCCGTGTGATC
TGAAACAGTGCCAAGCAGCCATACGGTTCTTCTATCAAAATATACCTTTTAACATTGCCCAGATGTTGGC
TTTTTGTGACTGTGCTCAATCTGATATACCTTGTCAGCAGTCCAAAGAAGCTCTTCACAGCAAGACATGT
GCAGTGAACATGGTTCCACCCCCTACTTGCCTCAGTGTAATTCGCAGCTGCCAAAATGATGAATTATGCA
GGAGGCACTATAGAACATTTCAGTCAAAATGCTGGCAGCGTGTGACTAGAAAGTGCCATGAAGATGAGAA
TTGCATTAGCACCTTAAGCAAACAGGACCTCACTTGTTCAGGAAGTGATGACTGCAAAGCTGCTTACATA
GATATCCTTGGGACGGTCCTTCAAGTGCAATGTACCTGTAGGACCATTACACAAAGTGAGGAATCTTTGT
GTAAGATTTTCCAGCACATGCTTCATAGAAAATCATGTTTCAATTATCCAACCCTGTCTAATGTCAAAGG
CATGGCATTGTATACAAGAAAACATGCAAACAAAATCACTTTAACTGGATTTCATTCCCCCTTCAATGGA
GAAGTAATCTATGCTGCCATGTGCATGACAGTCACCTGTGGAATCCTTCTGTTGGTTATGGTCAAGCTTA
GAACTTCCAGAATATCAAGTAAAGCAAGAGATCCTTCACCGATCCAAATACCTGGAGAACTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG213001 representing NM_207410
Red=Cloning site Green=Tags(s)

MIVFIFLAMGLSLENEYTSQTNNCTYLREQCLRDANGCKHAWRVMEDACNDSDPGDPCKMRNSSYCNLSI
QYLVESNFQFKECLCTDDFYCTVNKLLGKKCINKSDNVKEDKFKWNLTTRSHHGFKGMWSCLEVAEACVG
DVVCNAQLASYLKACSANGNPCDLKQCQAAIRFFYQNIPFNIAQMLAFCDCAQSDIPCQQSKEALHSKTC
AVNMVPPPTCLSVIRSCQNDELCRRHYRTFQSKCWQRVTRKCHEDENCISTLSKQDLTCSGSDDCKAAYI
DILGTVLQVQCTCRTITQSEESLCKIFQHMLHRKSCFNYPTLSNVKGMALYTRKHANKITLTGFHSPFNG
EVIYAAMCMTVTCGILLLVMVKLRTSRISSKARDPSPIQIPGEL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_207410
ORF Size 1182 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_207410.1, NP_997293.1
RefSeq Size 1911 bp
RefSeq ORF 1185 bp
Locus ID 389400
UniProt ID Q6UXV0
Protein Families Druggable Genome, Transmembrane
Gene Summary Brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses (PubMed:28953886, PubMed:28846097, PubMed:28846098, PubMed:28846099). Upon interaction with its ligand, GDF15, interacts with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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