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Collagen V (COL5A1) (NM_000093) Human Tagged ORF Clone
CAT＃: RG212443
- TrueORF®
COL5A1 (tGFP-tagged) - Human collagen, type V, alpha 1 (COL5A1)
ORF Plasmid: DDK
"NM_000093" in other vectors (2)
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CNY 20,128.00
货期*
现货
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | EDSC; EDSCL1; FMDMF |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG212443 representing NM_000093
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTCCATACCCGCTGGAAAGCGCGCAGCGCGCTCCGCCCGGGCGCCCCGCTGCTGCCCCCGCTGC TGCTGCTGCTGCTGTGGGCGCCGCCTCCGAGCCGCGCAGCTCAGCCAGCAGATCTCCTGAAGGTTCTAGA TTTTCACAACTTGCCTGATGGAATAACAAAGACAACAGGCTTTTGCGCCACGCGGCGATCTTCCAAAGGC CCGGATGTCGCTTACAGAGTCACCAAAGACGCGCAGCTCAGCGCACCCACCAAGCAGCTGTACCCTGCGT CTGCATTTCCCGAGGACTTCTCCATCCTAACAACTGTGAAAGCCAAGAAAGGCAGCCAGGCCTTCCTGGT CTCCATCTACAACGAGCAGGGTATCCAGCAGATTGGGCTGGAGCTGGGCCGCTCTCCCGTCTTCCTCTAC GAGGACCACACGGGGAAGCCTGGCCCGGAAGACTACCCCCTCTTCCGGGGCATCAACCTGTCAGATGGCA AGTGGCACAGAATTGCTCTCAGCGTCCACAAGAAAAATGTCACCTTGATCCTCGACTGTAAAAAGAAGAC CACCAAATTCCTCGACCGCAGCGACCACCCCATGATCGACATCAATGGCATCATCGTGTTTGGCACCCGG ATCCTGGATGAGGAGGTGTTTGAGGGTGACATCCAGCAGCTGCTCTTTGTCTCGGACCACCGGGCAGCTT ATGATTACTGTGAGCACTACAGCCCTGACTGTGACACCGCAGTACCTGACACCCCACAGTCGCAGGACCC CAATCCAGATGAATATTACACGGAAGGAGACGGCGAGGGTGAGACCTATTACTACGAATACCCCTACTAC GAAGACCCCGAAGACCTAGGGAAGGAGCCCACCCCCAGCAAGAAGCCCGTGGAAGCTGCCAAAGAAACCA CAGAGGTCCCCGAGGAGCTGACCCCGACCCCCACGGAAGCTGCTCCCATGCCTGAAACCAGTGAAGGGGC TGGGAAGGAAGAGGACGTCGGCATCGGGGACTATGACTACGTGCCCAGTGAGGACTACTACACGCCCTCA CCGTATGATGACCTCACCTATGGCGAGGGGGAGGAGAACCCTGACCAGCCCACAGACCCAGGCGCTGGGG CCGAAATTCCCACCAGCACCGCCGACACCTCCAACTCCTCCAATCCAGCTCCGCCTCCAGGGGAAGGTGC GGATGACTTGGAGGGGGAGTTCACTGAGGAAACGATCCGGAACCTTGACGAGAACTACTACGACCCCTAC TACGACCCCACCAGCTCCCCGTCGGAGATCGGGCCGGGAATGCCGGCGAACCAGGATACCATCTATGAAG GGATTGGAGGACCTCGGGGCGAGAAAGGCCAAAAGGGAGAACCAGCGATTATCGAGCCGGGCATGCTCAT CGAGGGCCCGCCTGGCCCAGAAGGCCCCGCGGGTCTTCCCGGACCTCCAGGAACCATGGGTCCCACTGGC CAAGTCGGGGACCCTGGAGAAAGGGGCCCCCCTGGACGCCCAGGCCTTCCTGGGGCCGATGGCCTGCCCG GTCCTCCAGGAACCATGCTCATGCTGCCCTTCCGGTTTGGAGGTGGCGGCGATGCGGGCTCCAAAGGCCC CATGGTCTCAGCCCAGGAGTCCCAGGCGCAAGCCATTCTCCAGCAGGCCAGGTTGGCACTGAGGGGACCA GCTGGCCCGATGGGTCTCACAGGGAGACCTGGCCCTGTGGGTCCCCCTGGGAGCGGAGGTTTGAAGGGCG AGCCGGGAGACGTGGGGCCTCAGGGTCCTCGAGGTGTGCAAGGCCCGCCTGGTCCGGCCGGGAAGCCCGG AAGACGGGGTCGGGCTGGGAGTGATGGAGCCAGAGGAATGCCTGGACAAACTGGCCCCAAGGGTGACCGG GGTTTCGACGGCCTGGCTGGGTTGCCAGGCGAGAAGGGCCACAGGGGTGACCCTGGTCCTTCCGGCCCAC CAGGACCTCCGGGAGACGATGGAGAAAGGGGTGACGACGGAGAAGTTGGGCCCAGGGGGCTGCCTGGGGA GCCCGGGCCACGTGGTCTGCTTGGGCCGAAAGGGCCCCCAGGTCCTCCCGGACCTCCCGGTGTCACGGGT ATGGACGGCCAGCCGGGGCCAAAAGGAAATGTGGGTCCCCAGGGAGAGCCTGGCCCCCCAGGACAGCAGG GTAATCCAGGCGCCCAGGGTCTTCCAGGCCCCCAGGGTGCAATTGGTCCTCCAGGAGAAAAGGGTCCCTT GGGGAAACCAGGCCTTCCAGGAATGCCCGGTGCTGACGGACCCCCGGGACACCCTGGCAAAGAAGGCCCT CCAGGAGAGAAAGGAGGTCAGGGTCCACCTGGCCCCCAGGGTCCGATTGGCTACCCAGGTCCTCGAGGAG TCAAGGGGGCCGATGGCATCCGTGGTCTGAAGGGCACAAAGGGCGAGAAGGGTGAAGACGGCTTTCCTGG GTTTAAAGGAGACATGGGCATCAAGGGTGATCGGGGGGAGATCGGCCCACCCGGTCCCAGGGGAGAAGAT GGCCCTGAAGGCCCAAAGGGTCGCGGAGGTCCCAATGGTGACCCCGGTCCTCTGGGACCCCCTGGGGAGA AGGGAAAACTCGGAGTCCCAGGGTTACCAGGGTATCCAGGAAGACAAGGACCAAAGGGCTCTATTGGATT CCCTGGATTTCCTGGCGCCAATGGAGAGAAGGGCGGCAGGGGGACCCCTGGAAAGCCAGGACCGCGGGGG CAGCGAGGCCCAACGGGTCCGAGGGGTGAAAGAGGCCCCCGGGGCATCACTGGGAAGCCTGGCCCCAAGG GCAACTCCGGAGGTGACGGCCCAGCTGGCCCTCCTGGTGAACGGGGACCCAATGGACCCCAAGGACCCAC AGGATTTCCTGGACCAAAGGGCCCCCCTGGCCCTCCAGGCAAGGATGGACTCCCAGGACACCCTGGACAG AGAGGCGAGACTGGTTTCCAAGGCAAGACCGGCCCTCCAGGCCCCCCCGGCGTGGTCGGCCCTCAGGGTC CCACGGGAGAAACGGGCCCAATGGGTGAGCGTGGCCACCCTGGGCCCCCTGGACCCCCCGGTGAACAGGG GCTTCCGGGCCTTGCTGGAAAAGAAGGGACGAAGGGTGACCCAGGCCCTGCAGGCCTCCCTGGGAAAGAT GGCCCTCCAGGATTACGTGGTTTCCCTGGGGACCGAGGGCTTCCTGGTCCAGTGGGAGCTCTTGGACTGA AAGGCAATGAAGGGCCCCCTGGCCCACCAGGCCCTGCGGGATCTCCAGGGGAGAGAGGTCCAGCTGGAGC CGCTGGGCCCATCGGAATTCCAGGGAGACCTGGGCCCCAGGGACCCCCAGGGCCGGCAGGAGAGAAAGGG GCTCCTGGCGAGAAAGGCCCACAAGGCCCAGCTGGCCGAGACGGTCTCCAGGGGCCTGTGGGGCTCCCGG GTCCAGCTGGCCCTGTGGGTCCCCCTGGAGAAGACGGAGATAAGGGAGAGATCGGGGAGCCGGGGCAGAA AGGAAGCAAGGGGGACAAAGGAGAACAGGGTCCTCCTGGGCCTACAGGTCCTCAAGGCCCCATCGGACAG CCAGGCCCCTCTGGAGCTGACGGCGAGCCGGGGCCTCGGGGCCAGCAGGGCCTTTTCGGGCAGAAAGGTG ATGAAGGTCCCAGAGGCTTTCCTGGACCCCCTGGGCCAGTGGGGCTGCAGGGTTTGCCAGGACCTCCAGG CGAGAAGGGTGAGACAGGAGACGTGGGCCAGATGGGCCCCCCGGGTCCCCCTGGCCCCCGAGGACCCTCC GGAGCTCCAGGTGCTGATGGCCCACAAGGTCCCCCAGGTGGAATAGGAAACCCTGGTGCAGTGGGAGAGA AGGGCGAGCCTGGCGAAGCAGGTGAGCCTGGCCCTTCCGGGAGAAGCGGCCCCCCGGGACCCAAAGGAGA AAGGGGAGAGAAGGGCGAGTCAGGCCCTTCAGGTGCTGCCGGACCCCCTGGACCCAAAGGCCCTCCCGGA GATGATGGTCCCAAAGGCAGCCCTGGCCCAGTGGGTTTTCCTGGAGATCCTGGCCCCCCCGGAGAGCCTG GCCCCGCGGGTCAAGATGGTCCCCCTGGTGACAAAGGAGATGATGGTGAACCCGGGCAGACGGGATCCCC CGGCCCTACTGGTGAACCAGGTCCATCGGGGCCTCCAGGAAAAAGGGGTCCCCCAGGCCCCGCAGGCCCC GAAGGCAGACAGGGAGAGAAAGGGGCCAAGGGAGAAGCCGGCTTGGAAGGCCCTCCTGGGAAGACTGGCC CCATCGGCCCCCAGGGGGCCCCTGGGAAGCCCGGACCGGATGGCCTTCGAGGGATCCCTGGCCCTGTGGG AGAACAAGGTCTCCCAGGATCCCCAGGCCCGGACGGTCCCCCCGGCCCCATGGGTCCCCCAGGACTTCCC GGCCTCAAAGGAGATTCTGGTCCCAAAGGTGAAAAGGGTCATCCAGGCCTGATCGGGCTCATCGGTCCTC CGGGTGAACAGGGTGAGAAGGGCGACCGTGGTCTCCCTGGCCCCCAGGGCTCCTCCGGTCCTAAGGGAGA ACAGGGTATCACTGGTCCTTCTGGCCCGATTGGGCCTCCTGGGCCCCCTGGCCTGCCGGGTCCGCCTGGT CCAAAAGGTGCTAAGGGCTCCTCGGGTCCAACTGGCCCGAAGGGTGAGGCAGGCCACCCAGGACCCCCAG GCCCCCCGGGCCCCCCGGGAGAGGTCATCCAGCCCCTGCCAATCCAGGCATCCAGGACGCGGCGGAACAT CGACGCCAGCCAGCTGCTGGACGACGGGAATGGCGAGAACTACGTGGACTACGCGGACGGCATGGAAGAG ATCTTCGGCTCTCTCAACTCTCTGAAGCTGGAGATTGAGCAGATGAAACGGCCCCTGGGCACGCAGCAGA ACCCCGCCCGCACCTGCAAGGACCTGCAGCTCTGCCACCCCGACTTCCCAGATGGTGAATACTGGGTCGA TCCTAACCAAGGATGCTCCAGGGATTCCTTCAAGGTTTACTGCAACTTCACAGCCGGGGGGTCGACATGC GTCTTCCCTGACAAGAAGTCCGAAGGGGCCAGAATCACTTCTTGGCCCAAAGAAAACCCGGGCTCCTGGT TCAGTGAATTCAAGCGTGGGAAACTGCTCTCCTATGTGGACGCCGAGGGCAACCCTGTGGGTGTGGTACA GATGACCTTCCTGCGGCTGCTGAGCGCCTCTGCCCACCAGAACGTCACCTACCACTGCTACCAGTCAGTG GCCTGGCAGGACGCAGCCACGGGCAGCTACGACAAGGCCCTCCGCTTCCTGGGCTCCAACGACGAGGAGA TGTCCTATGACAACAACCCCTACATCCGCGCCCTGGTGGACGGCTGTGCTACCAAGAAAGGCTACCAGAA GACGGTTCTGGAGATCGACACCCCCAAAGTGGAGCAGGTGCCCATCGTGGACATCATGTTCAATGACTTC GGTGAAGCGTCACAGAAATTTGGATTTGAAGTGGGGCCGGCTTGCTTCATGGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212443 representing NM_000093
Red=Cloning site Green=Tags(s) MDVHTRWKARSALRPGAPLLPPLLLLLLWAPPPSRAAQPADLLKVLDFHNLPDGITKTTGFCATRRSSKG PDVAYRVTKDAQLSAPTKQLYPASAFPEDFSILTTVKAKKGSQAFLVSIYNEQGIQQIGLELGRSPVFLY EDHTGKPGPEDYPLFRGINLSDGKWHRIALSVHKKNVTLILDCKKKTTKFLDRSDHPMIDINGIIVFGTR ILDEEVFEGDIQQLLFVSDHRAAYDYCEHYSPDCDTAVPDTPQSQDPNPDEYYTEGDGEGETYYYEYPYY EDPEDLGKEPTPSKKPVEAAKETTEVPEELTPTPTEAAPMPETSEGAGKEEDVGIGDYDYVPSEDYYTPS PYDDLTYGEGEENPDQPTDPGAGAEIPTSTADTSNSSNPAPPPGEGADDLEGEFTEETIRNLDENYYDPY YDPTSSPSEIGPGMPANQDTIYEGIGGPRGEKGQKGEPAIIEPGMLIEGPPGPEGPAGLPGPPGTMGPTG QVGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALRGP AGPMGLTGRPGPVGPPGSGGLKGEPGDVGPQGPRGVQGPPGPAGKPGRRGRAGSDGARGMPGQTGPKGDR GFDGLAGLPGEKGHRGDPGPSGPPGPPGDDGERGDDGEVGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTG MDGQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGKEGP PGEKGGQGPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRGED GPEGPKGRGGPNGDPGPLGPPGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGPRG QRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPPGPPGKDGLPGHPGQ RGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGLAGKEGTKGDPGPAGLPGKD GPPGLRGFPGDRGLPGPVGALGLKGNEGPPGPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKG APGEKGPQGPAGRDGLQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPIGQ PGPSGADGEPGPRGQQGLFGQKGDEGPRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPS GAPGADGPQGPPGGIGNPGAVGEKGEPGEAGEPGPSGRSGPPGPKGERGEKGESGPSGAAGPPGPKGPPG DDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPTGEPGPSGPPGKRGPPGPAGP EGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGLP GLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSSGPKGEQGITGPSGPIGPPGPPGLPGPPG PKGAKGSSGPTGPKGEAGHPGPPGPPGPPGEVIQPLPIQASRTRRNIDASQLLDDGNGENYVDYADGMEE IFGSLNSLKLEIEQMKRPLGTQQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTC VFPDKKSEGARITSWPKENPGSWFSEFKRGKLLSYVDAEGNPVGVVQMTFLRLLSASAHQNVTYHCYQSV AWQDAATGSYDKALRFLGSNDEEMSYDNNPYIRALVDGCATKKGYQKTVLEIDTPKVEQVPIVDIMFNDF GEASQKFGFEVGPACFMG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000093 |
| ORF Size | 5514 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000093.2, NP_000084.2 |
| RefSeq Size | 6496 bp |
| RefSeq ORF | 5517 bp |
| Locus ID | 1289 |
| UniProt ID | P20908 |
| Protein Families | Druggable Genome |
| Protein Pathways | ECM-receptor interaction, Focal adhesion |
| Gene Summary | This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013] |
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