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REST (NM_005612) Human Tagged ORF Clone
CAT＃: RG211570
- TrueORF®
REST (tGFP-tagged) - Human RE1-silencing transcription factor (REST), transcript variant 1
ORF Plasmid: DDK
"NM_005612" in other vectors (7)
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CNY 13,056.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DFNA27; GINGF5; HGF5; NRSF; WT6; XBR |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG211570 representing NM_005612
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCCAGGTAATGGGGCAGTCTTCTGGAGGAGGAGGGCTGTTTACCAGCAGTGGCAACATTGGAA TGGCCCTGCCTAACGACATGTATGACTTGCATGACCTTTCCAAAGCTGAACTGGCCGCACCTCAGCTTAT TATGCTGGCAAATGTGGCCTTAACTGGGGAAGTAAATGGCAGCTGCTGTGATTACCTGGTCGGTGAAGAA AGACAGATGGCAGAACTGATGCCGGTTGGGGATAACAACTTTTCAGATAGTGAAGAAGGAGAAGGACTTG AAGAGTCTGCTGATATAAAAGGTGAACCTCATGGACTGGAAAACATGGAACTGAGAAGTTTGGAACTCAG CGTCGTAGAACCTCAGCCTGTATTTGAGGCATCAGGTGCTCCAGATATTTACAGTTCAAATAAAGATCTT CCCCCTGAAACACCTGGAGCGGAGGACAAAGGCAAGAGCTCGAAGACCAAACCCTTTCGCTGTAAGCCAT GCCAATATGAAGCAGAATCTGAAGAACAGTTTGTGCATCACATCAGAGTTCACAGTGCTAAGAAATTTTT TGTGGAAGAGAGTGCAGAGAAGCAGGCAAAAGCCAGGGAATCTGGCTCTTCCACTGCAGAAGAGGGAGAT TTCTCCAAGGGCCCCATTCGCTGTGACCGCTGCGGCTACAATACTAATCGATATGATCACTATACAGCAC ACCTGAAACACCACACCAGAGCTGGGGATAATGAGCGAGTCTACAAGTGTATCATTTGCACATACACAAC AGTGAGCGAGTATCACTGGAGGAAACATTTAAGAAACCATTTTCCAAGGAAAGTATACACATGTGGAAAA TGCAACTATTTTTCAGACAGAAAAAACAATTATGTTCAGCATGTTAGAACTCATACAGGAGAACGCCCAT ATAAATGTGAACTTTGTCCTTACTCAAGTTCTCAGAAGACTCATCTAACTAGACATATGCGTACTCATTC AGGTGAGAAGCCATTTAAATGTGATCAGTGCAGTTATGTGGCCTCTAATCAACATGAAGTAACCCGCCAT GCAAGACAGGTTCACAATGGGCCTAAACCTCTTAATTGCCCACACTGTGATTACAAAACAGCAGATAGAA GCAACTTCAAAAAACATGTAGAGCTACATGTGAACCCACGGCAGTTCAATTGCCCTGTATGTGACTATGC AGCTTCCAAGAAGTGTAATCTACAGTATCACTTCAAATCTAAGCATCCTACTTGTCCTAATAAAACAATG GATGTCTCAAAAGTGAAACTAAAGAAAACCAAAAAACGAGAGGCTGACTTGCCTGATAATATTACCAATG AAAAAACAGAAATAGAACAAACAAAAATAAAAGGGGATGTGGCTGGAAAGAAAAATGAAAAGTCCGTCAA AGCAGAGAAAAGAGATGTCTCAAAAGAGAAAAAGCCTTCTAATAATGTGTCAGTGATCCAGGTGACTACC AGAACTCGAAAATCAGTAACAGAGGTGAAAGAGATGGATGTGCATACAGGAAGCAATTCAGAAAAATTCA GTAAAACTAAGAAAAGCAAAAGGAAGCTGGAAGTTGACAGCCATTCTTTACATGGTCCTGTGAATGATGA GGAATCTTCAACAAAAAAGAAAAAGAAGGTAGAAAGCAAATCCAAAAATAATAGTCAGGAAGTGCCAAAG GGTGACAGCAAAGTGGAGGAGAATAAAAAGCAAAATACTTGCATGAAAAAAAGTACAAAGAAGAAAACTC TGAAAAATAAATCAAGTAAGAAAAGCAGTAAGCCTCCTCAGAAGGAACCTGTTGAGAAGGGATCTGCTCA GATGGACCCTCCTCAGATGGGGCCTGCTCCCACAGAGGCGGTTCAGAAGGGGCCCGTTCAGGTGGAGCTG CCACCTCCCATGGAGCATGCTCAGATGGAGGGTGCCCAGATACGGCCTGCTCCTGACGAGCCTGTTCAGA TGGAGGTGGTTCAGGAGGGGCCTGCTCAGAAGGAGCTGCTGCCTCCCGTGGAGCCTGCTCAGATGGTGGG TGCCCAAATTGTACTTGCTCACATGGAGCTGCCTCCTCCCATGGAGACTGCTCAGACGGAGGTTGCCCAA ATGGGGCCTGCTCCCATGGAACCTGCTCAGATGGAGGTTGCCCAGGTAGAATCTGCTCCCATGCAGGTGG TCCAGAAGGAGCCTGTTCAGATGGAGCTGTCTCCTCCCATGGAGGTGGTCCAGAAGGAGCCTGTTCAGAT AGAGCTGTCTCCTCCCATGGAGGTGGTCCAGAAGGAACCTGTTAAGATAGAGCTGTCTCCTCCCATAGAG GTGGTCCAGAAGGAGCCTGTTCAGATGGAGTTGTCTCCTCCCATGGGGGTGGTTCAGAAGGAGCCTGCTC AGAGGGAGCCACCTCCTCCCAGAGAGCCTCCCCTTCACATGGAGCCAATTTCCAAAAAGCCTCCTCTCCG AAAAGATAAAAAGGAAAAGTCTAACATGCAGAGTGAAAGGGCACGGAAGGAGCAAGTCCTTATTGAAGTT GGCTTAGTGCCTGTTAAAGATAGCTGGCTTCTAAAGGAAAGTGTAAGCACAGAGGATCTCTCACCACCAT CACCACCACTGCCAAAGGAAAATTTAAGAGAAGAGGCATCAGGAGACCAAAAATTACTCAACACAGGTGA AGGAAATAAAGAAGCCCCTCTTCAGAAAGTAGGAGCAGAAGAGGCAGATGAGAGCCTACCTGGTCTTGCT GCTAATATCAACGAATCTACCCATATTTCATCCTCTGGACAAAACTTGAATACGCCAGAGGGTGAAACTT TAAATGGTAAACATCAGACTGACAGTATAGTTTGTGAAATGAAAATGGACACTGATCAGAACACAAGAGA GAATCTCACTGGTATAAATTCAACAGTTGAAGAACCAGTTTCACCAATGCTTCCCCCTTCAGCAGTAGAA GAACGTGAAGCAGTGTCCAAAACTGCACTGGCATCACCTCCTGCTACAATGGCAGCAAATGAGTCTCAGG AAATTGATGAAGATGAAGGCATCCACAGCCATGAAGGAAGTGACCTAAGTGACAACATGTCAGAGGGTAG TGATGATTCTGGATTGCATGGGGCTCGGCCAGTTCCACAAGAATCTAGCAGAAAAAATGCAAAGGAAGCC TTGGCAGTCAAAGCGGCTAAGGGAGATTTTGTTTGTATCTTCTGTGATCGTTCTTTCAGAAAGGGAAAAG ATTACAGCAAACACCTCAATCGCCATTTGGTTAATGTGTACTATCTTGAAGAAGCAGCTCAAGGGCAGGA G ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG211570 representing NM_005612
Red=Cloning site Green=Tags(s) MATQVMGQSSGGGGLFTSSGNIGMALPNDMYDLHDLSKAELAAPQLIMLANVALTGEVNGSCCDYLVGEE RQMAELMPVGDNNFSDSEEGEGLEESADIKGEPHGLENMELRSLELSVVEPQPVFEASGAPDIYSSNKDL PPETPGAEDKGKSSKTKPFRCKPCQYEAESEEQFVHHIRVHSAKKFFVEESAEKQAKARESGSSTAEEGD FSKGPIRCDRCGYNTNRYDHYTAHLKHHTRAGDNERVYKCIICTYTTVSEYHWRKHLRNHFPRKVYTCGK CNYFSDRKNNYVQHVRTHTGERPYKCELCPYSSSQKTHLTRHMRTHSGEKPFKCDQCSYVASNQHEVTRH ARQVHNGPKPLNCPHCDYKTADRSNFKKHVELHVNPRQFNCPVCDYAASKKCNLQYHFKSKHPTCPNKTM DVSKVKLKKTKKREADLPDNITNEKTEIEQTKIKGDVAGKKNEKSVKAEKRDVSKEKKPSNNVSVIQVTT RTRKSVTEVKEMDVHTGSNSEKFSKTKKSKRKLEVDSHSLHGPVNDEESSTKKKKKVESKSKNNSQEVPK GDSKVEENKKQNTCMKKSTKKKTLKNKSSKKSSKPPQKEPVEKGSAQMDPPQMGPAPTEAVQKGPVQVEL PPPMEHAQMEGAQIRPAPDEPVQMEVVQEGPAQKELLPPVEPAQMVGAQIVLAHMELPPPMETAQTEVAQ MGPAPMEPAQMEVAQVESAPMQVVQKEPVQMELSPPMEVVQKEPVQIELSPPMEVVQKEPVKIELSPPIE VVQKEPVQMELSPPMGVVQKEPAQREPPPPREPPLHMEPISKKPPLRKDKKEKSNMQSERARKEQVLIEV GLVPVKDSWLLKESVSTEDLSPPSPPLPKENLREEASGDQKLLNTGEGNKEAPLQKVGAEEADESLPGLA ANINESTHISSSGQNLNTPEGETLNGKHQTDSIVCEMKMDTDQNTRENLTGINSTVEEPVSPMLPPSAVE EREAVSKTALASPPATMAANESQEIDEDEGIHSHEGSDLSDNMSEGSDDSGLHGARPVPQESSRKNAKEA LAVKAAKGDFVCIFCDRSFRKGKDYSKHLNRHLVNVYYLEEAAQGQE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005612 |
| ORF Size | 3291 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005612.3, NP_005603.2 |
| RefSeq Size | 3663 bp |
| RefSeq ORF | 3294 bp |
| Locus ID | 5978 |
| UniProt ID | Q13127 |
| Domains | zf-C2H2 |
| Protein Families | Transcription Factors |
| Protein Pathways | Huntington's disease |
| Gene Summary | This gene was initially identified as a transcriptional repressor that represses neuronal genes in non-neuronal tissues. However, depending on the cellular context, this gene can act as either an oncogene or a tumor suppressor. The encoded protein is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regulator of neurogenesis. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2018] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Neuroendocrine Key Regulator Gene Expression in Merkel Cell Carcinoma
,Chteinberg, E;Sauer, CM;Rennspiess, D;Beumers, L;Schiffelers, L;Eben, J;Haugg, A;Winnepenninckx, V;Kurz, AK;Speel, EJ;Zenke, M;Zur Hausen, A;,
Neoplasia
,PubMed ID 30414538
[REST]
|
|
REST is Required for Nuclear Reprogramming by Inhibiting TGFß Signaling Pathway
,Kong, QR;Xie, BT;Zhang, H;Li, JY;Huang, TQ;Wei, RY;Liu, ZH;,
J. Biol. Chem.
,PubMed ID 27821591
[REST]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211570 | REST (Myc-DDK-tagged)-Human RE1-silencing transcription factor (REST), transcript variant 1 |
CNY 11,456.00 |
|
| RC211570L1 | Lenti ORF clone of Human RE1-silencing transcription factor (REST), transcript variant 1, Myc-DDK-tagged |
CNY 13,856.00 |
|
| RC211570L2 | Lenti ORF clone of Human RE1-silencing transcription factor (REST), transcript variant 1, mGFP tagged |
CNY 13,856.00 |
|
| RC211570L3 | Lenti ORF clone of Human RE1-silencing transcription factor (REST), transcript variant 1, Myc-DDK-tagged |
CNY 10,260.00 |
|
| RC211570L4 | Lenti ORF clone of Human RE1-silencing transcription factor (REST), transcript variant 1, mGFP tagged |
CNY 13,856.00 |
|
| SC116634 | REST (untagged)-Human RE1-silencing transcription factor (REST), transcript variant 1 |
CNY 11,376.00 |
|
| SC318496 | REST (untagged)-Human RE1-silencing transcription factor (REST), transcript variant 1 |
CNY 11,464.00 |
