REST (NM_005612) Human Tagged Lenti ORF Clone
CAT#: RC211570L4
- LentiORF®
Lenti ORF clone of Human RE1-silencing transcription factor (REST), transcript variant 1, mGFP tagged
"NM_005612" in other vectors (7)
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CNY 13,856.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Synonyms | DFNA27; GINGF5; HGF5; NRSF; WT6; XBR |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC211570).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_005612 |
ORF Size | 3291 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005612.3 |
RefSeq Size | 7333 bp |
RefSeq ORF | 3294 bp |
Locus ID | 5978 |
UniProt ID | Q13127 |
Domains | zf-C2H2 |
Protein Families | Transcription Factors |
Protein Pathways | Huntington's disease |
MW | 121.9 kDa |
Gene Summary | This gene was initially identified as a transcriptional repressor that represses neuronal genes in non-neuronal tissues. However, depending on the cellular context, this gene can act as either an oncogene or a tumor suppressor. The encoded protein is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regulator of neurogenesis. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2018] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC211570 | REST (Myc-DDK-tagged)-Human RE1-silencing transcription factor (REST), transcript variant 1 |
CNY 11,456.00 |
|
RC211570L1 | Lenti ORF clone of Human RE1-silencing transcription factor (REST), transcript variant 1, Myc-DDK-tagged |
CNY 13,856.00 |
|
RC211570L2 | Lenti ORF clone of Human RE1-silencing transcription factor (REST), transcript variant 1, mGFP tagged |
CNY 13,856.00 |
|
RC211570L3 | Lenti ORF clone of Human RE1-silencing transcription factor (REST), transcript variant 1, Myc-DDK-tagged |
CNY 10,260.00 |
|
RG211570 | REST (tGFP-tagged) - Human RE1-silencing transcription factor (REST), transcript variant 1 |
CNY 13,056.00 |
|
SC116634 | REST (untagged)-Human RE1-silencing transcription factor (REST), transcript variant 1 |
CNY 11,376.00 |
|
SC318496 | REST (untagged)-Human RE1-silencing transcription factor (REST), transcript variant 1 |
CNY 11,464.00 |