EI24 (NM_001007277) Human Tagged ORF Clone

CAT#: RG211554

  • TrueORF®

EI24 (tGFP-tagged) - Human etoposide induced 2.4 mRNA (EI24), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_001007277" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

CNY 4,370.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-EI24 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms EPG4; PIG8; TP53I8
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG211554 representing NM_001007277
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGACAGTGTCAAAACCTTTCTCCAGGACCTTGCCAGAGGAATCAAAGACTCCATCTGGGGTATTT
GTACCATCTCAAAGCTAGATGCTCGAATCCAGCAAAAGAGAGAGGAGCAGCGTCGAAGAAGGGCAAGTAG
TGTCTTGGCACAGAGAAGAGCCCAGAGTATAGAGCGGAAGCAAGAGAGTGAGCCACGTATTGTTAGTAGA
ATTTTCCAGTGTTGTGCTTGGAATGGTGGAGTGTTCTGGTTCAGTCTCCTCTTGTTTTATCGAGTATTTA
TTCCTGTGCTTCAGTCGGTAACAGCCCGAATTATCGGTGACCCATCACTACATGGAGATGTTTGGTCGTG
GCTGGAATTCTTCCTCACGTCAATTTTCAGTGCTCTTTGGGTGCTCCCCTTGTTTGTGCTTAGCAAAGTG
GTGAATGCCATTTGGTTTCAGGATATAGCTGACCTGGCATTTGAGGTATCAGGGAGGAAGCCTCACCCAT
TCCCTAGTGTCAGCAAAATAATTGCTGACATGCTCTTCAACCTTTTGCTGCAGGCTCTTTTCCTCATTCA
GGGAATGTTTGTGAGTCTCTTTCCCATCCATCTTGTCGGTCAGCTGGTTAGTCTCCTGCATATGTCCCTT
CTCTACTCACTGTACTGCTTTGAATATCGTTGGTTCAATAAAGTGGCTGCCTTTTCTCTATCCTCTTTCC
TTTATTCATTATCAGCGCCAATGAAGCAAAGACCCCTGGCAAAGCATATCTCTTCCAGTTGCGCCTCTTC
TCCTTGGTGGTCTTCT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG211554 representing NM_001007277
Red=Cloning site Green=Tags(s)

MADSVKTFLQDLARGIKDSIWGICTISKLDARIQQKREEQRRRRASSVLAQRRAQSIERKQESEPRIVSR
IFQCCAWNGGVFWFSLLLFYRVFIPVLQSVTARIIGDPSLHGDVWSWLEFFLTSIFSALWVLPLFVLSKV
VNAIWFQDIADLAFEVSGRKPHPFPSVSKIIADMLFNLLLQALFLIQGMFVSLFPIHLVGQLVSLLHMSL
LYSLYCFEYRWFNKVAAFSLSSFLYSLSAPMKQRPLAKHISSSCASSPWWSS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001007277
ORF Size 786 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001007277.1, NP_001007278.1
RefSeq Size 2167 bp
RefSeq ORF 788 bp
Locus ID 9538
Protein Families Druggable Genome, Transmembrane
Protein Pathways p53 signaling pathway
Gene Summary This gene encodes a putative tumor suppressor and has higher expression in p53-expressing cells than in control cells and is an immediate-early induction target of p53-mediated apoptosis. The encoded protein may suppress cell growth by inducing apoptotic cell death through the caspase 9 and mitochondrial pathways. This gene is located on human chromosome 11q24, a region frequently altered in cancers. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 1, 3, 7, and 8. [provided by RefSeq, Feb 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...