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PP4R1 (PPP4R1) (NM_005134) Human Tagged ORF Clone
CAT＃: RG209255
- TrueORF®
PPP4R1 (tGFP-tagged) - Human protein phosphatase 4, regulatory subunit 1 (PPP4R1), transcript variant 2
ORF Plasmid: DDK
"NM_005134" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MEG1; PP4(Rmeg); PP4R1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG209255 representing NM_005134
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACCTCTCGCTGCTTCAGGAGGACCTGCAGGAGGACGCAGACGGATCCCTGGACTTTGTCTCAC AAGATGAAATGTTGACGCCCCTGGGGAGATTGGACAAGTATGCTGCAAGTGAGAACATATTTAACAGACA AATGGTGGCCCGGAGTTTGCTCGATACCTTGAGGGAAGTCTGCGATGATGAAAGAGATTGTATTGCTGTT TTGGAAAGAATTAGCAGATTGGCCGATGATTCAGAACCAACTGTGAGAGCGGAGCTGATGGAACAGGTGC CTCACATCGCACTGTTTTGTCAAGAAAACCGGCCTTCAATACCATATGCTTTTTCAAAATTCTTACTACC TATTGTGGTTAGATACCTTGCAGATCAGAATAATCAGGTGAGGAAAACAAGTCAGGCAGCTTTGCTGGCT CTGTTGGAGCAGGAGCTCATTGAACGATTTGATGTGGAGACCAAAGTGTGCCCTGTCCTCATAGAGCTGA CAGCCCCAGATAGCAATGATGATGTGAAAACAGAAGCTGTGGCTATAATGTGCAAAATGGCTCCCATGGT TGGGAAGGATATTACAGAGCGTCTTATCCTCCCTAGGTTTTGTGAGATGTGCTGCGATTGCAGAATGTTT CACGTTCGAAAGGTCTGTGCTGCCAATTTTGGAGATATTTGCAGTGTAGTTGGCCAGCAAGCTACTGAAG AAATGTTGCTGCCCAGATTTTTCCAGCTTTGTTCTGATAATGTATGGGGAGTCCGAAAGGCTTGTGCTGA ATGCTTCATGGCGGTTTCATGTGCAACATGTCAAGAAATCCGACGGACCAAATTATCAGCACTTTTTATT AATTTGATCAGTGATCCTTCACGTTGGGTTCGCCAAGCAGCTTTTCAGTCTCTGGGACCTTTCATATCTA CTTTTGCTAATCCATCTAGCTCAGGCCAGTATTTTAAAGAAGAAAGCAAAAGTTCAGAAGAGATGTCAGT AGAAAACAAAAATAGGACCAGAGATCAAGAAGCCCCAGAGGATGTACAAGTCAGGCCAGAGGATACTCCT TCAGATCTCAGTGTTAGTAATTCCAGTGTCATACTGGAAAACACGATGGAAGACCATGCTGCTGAGGCAT CCGGGAAGCCTCTAGGTGAAATTAGTGTTCCACTGGACAGCTCTTTACTTTGTACTTTGTCCTCAGAATC TCACCAGGAAGCAGCTAGTAATGAGAATGATAAAAAACCTGGTAACTACAAATCTATGTTACGACCAGAG GTTGGCACCACTTCACAAGATTCAGCTCTCTTAGATCAGGAATTGTATAACTCCTTCCATTTCTGGAGGA CTCCTCTTCCTGAAATAGATCTAGACATAGAGCTTGAACAGAACTCTGGGGGAAAACCCAGCCCAGAGGG ACCAGAGGAAGAATCTGAGGGCCCTGTGCCCAGTTCTCCAAACATCACCATGGCCACCAGAAAGGAACTG GAAGAAATGATAGAAAATCTAGAGCCCCACATTGATGATCCAGATGTTAAAGCACAAGTGGAAGTGCTGT CCGCTGCACTACGTGCTTCCAGCCTGGATGCACATGAAGAGACCATCAGTATAGAAAAGAGAAGTGATTT GCAAGATGAACTGGATATAAATGAGCTACCAAATTGTAAAATAAATCAAGAAGATTCTGTGCCTTTAATC AGCGATGCTGTTGAGAATATGGACTCCACTCTTCACTATATTCACAGCGATTCAGACTTGAGCAACAATA GCAGTTTTAGCCCTGATGAGGAAAGGAGAACTAAAGTACAAGATGTTGTACCTCAGGCGTTGTTAGATCA GTATTTATCTATGACTGACCCTTCTCGTGCACAGACGGTTGACACTGAAATTGCTAAGCACTGTGCATAT AGCCTCCCTGGTGTGGCCTTGACACTCGGAAGACAGAATTGGCACTGCCTGAGAGAGACGTATGAGACTC TGGCCTCAGACATGCAGTGGAAAGTTCGACGAACTCTAGCATTCTCCATCCACGAGCTTGCAGTTATTCT TGGAGATCAATTGACAGCTGCAGATCTGGTTCCAATTTTTAATGGATTTTTAAAAGACCTCGATGAAGTC AGGATAGGTGTTCTTAAACACTTGCATGATTTTCTGAAGCTTCTTCATATTGACAAAAGAAGAGAATATC TTTATCAACTTCAGGAGTTTTTGGTGACAGATAATAGTAGAAATTGGCGGTTTCGAGCTGAACTGGCTGA ACAGCTGATTTTACTTCTAGAGTTATATAGTCCCAGAGATGTTTATGACTATTTACGTCCCATTGCTCTG AATCTGTGTGCAGACAAAGTTTCTTCTGTTCGTTGGATTTCCTACAAGTTGGTCAGCGAGATGGTGAAGA AGCTGCACGCGGCAACACCACCAACGTTCGGAGTGGACCTCATCAATGAGCTTGTGGAGAACTTTGGCAG ATGTCCCAAGTGGTCTGGTCGGCAAGCCTTTGTCTTTGTCTGCCAGACTGTCATTGAGGATGACTGCCTT CCCATGGACCAGTTTGCTGTGCATCTCATGCCGCATCTGCTAACCTTAGCAAATGACAGGGTTCCTAACG TGCGAGTGCTGCTTGCAAAGACATTAAGACAAACTCTACTAGAAAAAGACTATTTCTTGGCCTCTGCCAG CTGCCACCAGGAGGCTGTGGAGCAGACCATCATGGCTCTTCAGATGGACCGTGACAGCGATGTCAAGTAT TTTGCAAGCATCCACCCTGCCAGTACCAAAATCTCCGAAGATGCCATGAGCACAGCGTCCTCAACCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209255 representing NM_005134
Red=Cloning site Green=Tags(s) MADLSLLQEDLQEDADGSLDFVSQDEMLTPLGRLDKYAASENIFNRQMVARSLLDTLREVCDDERDCIAV LERISRLADDSEPTVRAELMEQVPHIALFCQENRPSIPYAFSKFLLPIVVRYLADQNNQVRKTSQAALLA LLEQELIERFDVETKVCPVLIELTAPDSNDDVKTEAVAIMCKMAPMVGKDITERLILPRFCEMCCDCRMF HVRKVCAANFGDICSVVGQQATEEMLLPRFFQLCSDNVWGVRKACAECFMAVSCATCQEIRRTKLSALFI NLISDPSRWVRQAAFQSLGPFISTFANPSSSGQYFKEESKSSEEMSVENKNRTRDQEAPEDVQVRPEDTP SDLSVSNSSVILENTMEDHAAEASGKPLGEISVPLDSSLLCTLSSESHQEAASNENDKKPGNYKSMLRPE VGTTSQDSALLDQELYNSFHFWRTPLPEIDLDIELEQNSGGKPSPEGPEEESEGPVPSSPNITMATRKEL EEMIENLEPHIDDPDVKAQVEVLSAALRASSLDAHEETISIEKRSDLQDELDINELPNCKINQEDSVPLI SDAVENMDSTLHYIHSDSDLSNNSSFSPDEERRTKVQDVVPQALLDQYLSMTDPSRAQTVDTEIAKHCAY SLPGVALTLGRQNWHCLRETYETLASDMQWKVRRTLAFSIHELAVILGDQLTAADLVPIFNGFLKDLDEV RIGVLKHLHDFLKLLHIDKRREYLYQLQEFLVTDNSRNWRFRAELAEQLILLLELYSPRDVYDYLRPIAL NLCADKVSSVRWISYKLVSEMVKKLHAATPPTFGVDLINELVENFGRCPKWSGRQAFVFVCQTVIEDDCL PMDQFAVHLMPHLLTLANDRVPNVRVLLAKTLRQTLLEKDYFLASASCHQEAVEQTIMALQMDRDSDVKY FASIHPASTKISEDAMSTASSTY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005134 |
| ORF Size | 2799 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005134.4 |
| RefSeq Size | 3878 bp |
| RefSeq ORF | 2802 bp |
| Locus ID | 9989 |
| UniProt ID | Q8TF05 |
| Domains | HEAT |
| Protein Families | Druggable Genome, Phosphatase |
| Gene Summary | This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4). The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470). There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209255 | PPP4R1 (Myc-DDK-tagged)-Human protein phosphatase 4, regulatory subunit 1 (PPP4R1), transcript variant 2 |
CNY 3,990.00 |
|
| RC209255L3 | Lenti ORF clone of Human protein phosphatase 4, regulatory subunit 1 (PPP4R1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209255L4 | Lenti ORF clone of Human protein phosphatase 4, regulatory subunit 1 (PPP4R1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| SC116949 | PPP4R1 (untagged)-Human protein phosphatase 4, regulatory subunit 1 (PPP4R1), transcript variant 2 |
CNY 2,950.00 |
