KCNK12 (NM_022055) Human Tagged ORF Clone

CAT#: RG208379

  • TrueORF®

KCNK12 (tGFP-tagged) - Human potassium channel, subfamily K, member 12 (KCNK12)

ORF Plasmid: DDK tGFP



  "NM_022055" in other vectors (6)

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CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal KCNK12 Antibody
    • 100 ug

CNY 5,808.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms K2p12.1; THIK-2; THIK2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG208379 representing NM_022055
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCTCCCGCAGCCCCCGGCCCCCGCCCCGCCGTAGCCGCCGCCGCCTGCCGCGCCCCTCCTGCTGCT
GCTGCTGCTGCCGCCGTTCGCACCTCAACGAGGACACCGGCCGCTTCGTGCTGCTGGCGGCGCTCATCGG
CCTCTACCTGGTGGCGGGTGCCACAGTCTTCTCGGCGCTCGAGAGCCCCGGCGAGGCGGAGGCGCGGGCG
CGCTGGGGCGCCACGCTGCGCAACTTCAGCGCTGCGCACGGCGTGGCCGAGCCAGAGCTGCGCGCCTTCC
TCCGGCACTACGAGGCCGCGCTGGCCGCCGGCGTCCGCGCCGACGCGCTGCGCCCGCGCTGGGACTTCCC
CGGCGCCTTCTACTTCGTGGGCACCGTGGTGTCAACCATAGGTTTCGGCATGACCACCCCCGCGACGGTG
GGCGGGAAGGCCTTCCTCATCGCCTACGGGCTGTTCGGCTGCGCTGGGACCATCCTGTTCTTCAACCTCT
TCCTGGAGCGCATCATCTCGCTGCTGGCCTTCATCATGCGCGCCTGCCGGGAGCGCCAGCTGCGCCGCAG
CGGCCTGCTGCCCGCCACCTTCCGCCGCGGCTCCGCGCTCTCGGAGGCCGACAGCCTGGCGGGCTGGAAG
CCCTCGGTGTACCACGTGCTGCTCATCCTGGGCCTGTTCGCCGTGCTGCTGTCCTGCTGCGCCTCGGCCA
TGTACACCAGCGTGGAGGGCTGGGACTACGTGGACTCGCTCTACTTCTGCTTCGTCACCTTCAGCACCAT
CGGCTTCGGGGACCTGGTGAGCAGCCAGCACGCCGCCTACCGGAACCAGGGGCTCTACCGCCTGGGCAAC
TTCCTCTTCATCCTGCTCGGCGTGTGCTGCATTTACTCGCTCTTCAACGTCATCTCCATCCTCATCAAGC
AGGTGCTCAACTGGATGCTGCGCAAGCTGAGCTGCCGCTGCTGCGCGCGCTGCTGCCCGGCTCCTGGCGC
GCCCCTGGCCCGGCGCAATGCCATCACCCCAGGCTCCCGGCTGCGCCGCCGCCTGGCCGCGCTCGGCGCC
GACCCCGCGGCCCGCGACAGCGACGCCGAGGGCCGCCGCCTCTCGGGCGAGCTCATCTCCATGCGCGACC
TCACGGCCTCCAACAAGGTGTCGCTGGCGCTGCTGCAGAAGCAGCTGTCGGAGACGGCCAACGGCTACCC
GCGCAGCGTGTGCGTCAACACGCGCCAGAACGGCTTCTCGGGCGGCGTGGGCGCGCTGGGCATCATGAAC
AACCGGCTGGCCGAGACCAGCGCCTCCAGG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG208379 representing NM_022055
Red=Cloning site Green=Tags(s)

MSSRSPRPPPRRSRRRLPRPSCCCCCCRRSHLNEDTGRFVLLAALIGLYLVAGATVFSALESPGEAEARA
RWGATLRNFSAAHGVAEPELRAFLRHYEAALAAGVRADALRPRWDFPGAFYFVGTVVSTIGFGMTTPATV
GGKAFLIAYGLFGCAGTILFFNLFLERIISLLAFIMRACRERQLRRSGLLPATFRRGSALSEADSLAGWK
PSVYHVLLILGLFAVLLSCCASAMYTSVEGWDYVDSLYFCFVTFSTIGFGDLVSSQHAAYRNQGLYRLGN
FLFILLGVCCIYSLFNVISILIKQVLNWMLRKLSCRCCARCCPAPGAPLARRNAITPGSRLRRRLAALGA
DPAARDSDAEGRRLSGELISMRDLTASNKVSLALLQKQLSETANGYPRSVCVNTRQNGFSGGVGALGIMN
NRLAETSASR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_022055
ORF Size 1290 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_022055.1, NP_071338.1
RefSeq Size 1449 bp
RefSeq ORF 1293 bp
Locus ID 56660
UniProt ID Q9HB15
Protein Families Druggable Genome, Ion Channels: Potassium, Transmembrane
Gene Summary This gene encodes one of the members of the superfamily of potassium channel proteins containing two pore-forming P domains. The product of this gene has not been shown to be a functional channel, however, it may require other non-pore-forming proteins for activity. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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