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LSS (NM_002340) Human Tagged ORF Clone
CAT＃: RG207793
- TrueORF®
LSS (tGFP-tagged) - Human lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (LSS), transcript variant 1
ORF Plasmid: DDK
"NM_002340" in other vectors (5)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | APMR4; CTRCT44; HYPT14; OSC |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG207793 representing NM_002340
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGAGGGCACGTGTCTGCGGCGCCGAGGGGGCCCCTACAAGACCGAGCCCGCCACCGACCTCGGCC GCTGGCGACTCAACTGCGAGAGGGGCCGGCAGACGTGGACCTACCTGCAGGACGAGCGCGCCGGCCGCGA GCAGACCGGCCTGGAAGCCTACGCCCTGGGGCTGGACACCAAGAATTACTTTAAGGACTTGCCCAAAGCC CACACCGCCTTTGAGGGGGCTCTGAACGGGATGACATTTTACGTGGGGCTGCAGGCTGAGGATGGGCACT GGACGGGTGATTATGGTGGCCCACTTTTCCTCCTGCCAGGCCTCCTGATCACTTGCCACGTGGCACGCAT CCCTCTGCCAGCCGGATACAGAGAAGAGATTGTGCGGTACCTGCGGTCAGTGCAGCTCCCTGACGGTGGC TGGGGCCTGCACATTGAGGATAAGTCCACCGTGTTTGGGACTGCGCTCAACTATGTGTCTCTCAGAATTC TGGGTGTTGGGCCTGACGATCCTGACCTGGTACGAGCCCGGAACATTCTTCACAAGAAAGGTGGTGCTGT GGCCATCCCCTCCTGGGGGAAGTTCTGGCTGGCTGTCCTGAATGTTTACAGCTGGGAAGGCCTCAATACC CTGTTCCCAGAGATGTGGCTGTTTCCTGACTGGGCACCGGCACACCCCTCCACACTCTGGTGCCACTGCC GGCAGGTGTACCTGCCCATGAGCTACTGCTACGCCGTTCGGCTGAGTGCCGCGGAAGACCCGCTGGTCCA GAGCCTCCGCCAGGAGCTCTATGTGGAGGACTTCGCCAGCATTGACTGGCTGGCGCAGAGGAACAACGTG GCCCCCGACGAGCTGTACACGCCGCACAGCTGGCTGCTCCGCGTGGTATATGCGCTCCTCAACCTGTATG AGCACCACCACAGTGCCCACCTGCGGCAGCGGGCCGTGCAGAAGCTGTATGAACACATTGTGGCCGACGA CCGATTCACCAAGAGCATCAGCATCGGCCCGATCTCGAAAACCATCAACATGCTTGTGCGCTGGTATGTG GACGGGCCCGCCTCCACTGCCTTCCAGGAGCATGTCTCCAGAATCCCGGACTATCTCTGGATGGGCCTTG ACGGCATGAAAATGCAGGGCACCAACGGCTCACAGATCTGGGACACCGCATTCGCCATCCAGGCTCTGCT TGAGGCGGGCGGGCACCACAGGCCCGAGTTTTCGTCCTGCCTGCAGAAGGCTCATGAGTTCCTGAGGCTC TCACAGGTCCCAGATAACCCTCCCGACTACCAGAAGTACTACCGCCAGATGCGCAAGGGTGGCTTCTCCT TCAGTACGCTGGACTGCGGCTGGATCGTTTCTGACTGCACGGCTGAGGCCTTGAAGGCTGTGCTGCTCCT GCAGGAGAAGTGTCCCCATGTCACCGAGCACATCCCCAGAGAACGGCTCTGCGATGCTGTGGCTGTGCTG CTGAACATGAGAAATCCAGATGGAGGGTTCGCCACCTATGAGACCAAGCGTGGGGGGCACTTGCTGGAGC TGCTGAACCCCTCGGAGGTCTTCGGGGACATCATGATTGACTACACCTATGTGGAGTGCACCTCAGCCGT GATGCAGGCGCTTAAGTATTTCCACAAGCGTTTCCCGGAGCACAGGGCAGCGGAGATCCGGGAGACCCTC ACGCAGGGCTTAGAGTTCTGTCGGCGGCAGCAGAGGGCCGATGGCTCCTGGGAAGGCTCCTGGGGAGTTT GCTTCACCTACGGCACCTGGTTTGGCCTGGAGGCCTTCGCCTGTATGGGGCAGACCTACCGAGATGGGAC TGCCTGTGCAGAGGTCTCCCGGGCCTGTGACTTCCTGCTGTCCCGGCAGATGGCAGACGGAGGCTGGGGG GAGGACTTTGAGTCCTGCGAGGAGCGGCGTTATTTGCAGAGTGCCCAGTCCCAGATCCATAACACATGCT GGGCCATGATGGGGCTGATGGCCGTTCGGCATCCTGACATCGAGGCCCAGGAGAGAGGAGTCCGGTGTCT ACTTGAGAAACAGCTCCCCAATGGCGACTGGCCGCAGGAAAACATTGCTGGGGTCTTCAACAAGTCCTGT GCCATCTCCTACACGAGCTACAGGAACATCTTCCCCATCTGGGCCCTCGGCCGCTTCTCCCAGCTGTACC CTGAGAGAGCCCTTGCTGGCCACCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207793 representing NM_002340
Red=Cloning site Green=Tags(s) MTEGTCLRRRGGPYKTEPATDLGRWRLNCERGRQTWTYLQDERAGREQTGLEAYALGLDTKNYFKDLPKA HTAFEGALNGMTFYVGLQAEDGHWTGDYGGPLFLLPGLLITCHVARIPLPAGYREEIVRYLRSVQLPDGG WGLHIEDKSTVFGTALNYVSLRILGVGPDDPDLVRARNILHKKGGAVAIPSWGKFWLAVLNVYSWEGLNT LFPEMWLFPDWAPAHPSTLWCHCRQVYLPMSYCYAVRLSAAEDPLVQSLRQELYVEDFASIDWLAQRNNV APDELYTPHSWLLRVVYALLNLYEHHHSAHLRQRAVQKLYEHIVADDRFTKSISIGPISKTINMLVRWYV DGPASTAFQEHVSRIPDYLWMGLDGMKMQGTNGSQIWDTAFAIQALLEAGGHHRPEFSSCLQKAHEFLRL SQVPDNPPDYQKYYRQMRKGGFSFSTLDCGWIVSDCTAEALKAVLLLQEKCPHVTEHIPRERLCDAVAVL LNMRNPDGGFATYETKRGGHLLELLNPSEVFGDIMIDYTYVECTSAVMQALKYFHKRFPEHRAAEIRETL TQGLEFCRRQQRADGSWEGSWGVCFTYGTWFGLEAFACMGQTYRDGTACAEVSRACDFLLSRQMADGGWG EDFESCEERRYLQSAQSQIHNTCWAMMGLMAVRHPDIEAQERGVRCLLEKQLPNGDWPQENIAGVFNKSC AISYTSYRNIFPIWALGRFSQLYPERALAGHP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002340 |
| ORF Size | 2196 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002340.6 |
| RefSeq Size | 4258 bp |
| RefSeq ORF | 2199 bp |
| Locus ID | 4047 |
| UniProt ID | P48449 |
| Domains | prenyltrans |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Steroid biosynthesis |
| Gene Summary | The protein encoded by this gene catalyzes the conversion of (S)-2,3 oxidosqualene to lanosterol. The encoded protein is a member of the terpene cyclase/mutase family and catalyzes the first step in the biosynthesis of cholesterol, steroid hormones, and vitamin D. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207793 | LSS (Myc-DDK-tagged)-Human lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (LSS), transcript variant 1 |
CNY 8,040.00 |
|
| RC207793L3 | Lenti-ORF clone of LSS (Myc-DDK-tagged)-Human lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (LSS), transcript variant 1 |
CNY 5,890.00 |
|
| RC207793L4 | Lenti-ORF clone of LSS (mGFP-tagged)-Human lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (LSS), transcript variant 1 |
CNY 5,890.00 |
|
| SC118683 | LSS (untagged)-Human lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (LSS), transcript variant 1 |
CNY 8,592.00 |
|
| SC322588 | LSS (untagged)-Human lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (LSS), transcript variant 1 |
CNY 8,056.00 |
