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PAPOLB (NM_020144) Human Tagged ORF Clone
CAT＃: RG205991
- TrueORF®
PAPOLB (tGFP-tagged) - Human poly(A) polymerase beta (testis specific) (PAPOLB)
ORF Plasmid: DDK
"NM_020144" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PAPT; TPAP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG205991 representing NM_020144
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCCGTTTCCGGTGACAACCCAGGGACCACCGCAGCCGGCGCCGCCGCCGAATCGCTACGGCGTCT CCTCGCCTATCAGTCTAGCGGTCCCCAAGGAGACGGACTGCCTCCTCACCCAGAGGCTAATAGAAACCCT CAGGCCCTTCGGGGTCTTCGAAGAGGAAGAGGAACTGCAGCGCAGGATTTTAGTTTTGGAAAAATTAAAT AATCTGGTAAAGGAATGGATACGCGAAATCAGTGAAAGCAAGAGTCTTCCCCAGTCTGTAATTGAAAACG TTGGAGGAAAGATTTTTACGTTTGGCTCTTACAGATTAGGAGTACATACGAAAGGCGCAGATATTGACGC CTTGTGCGTTGCACCAAGTCATGTGGATCGAAGCGACTTTTTCACCTCATTCTATGCTAAACTGAAACTA CAGGAGGAAGTGAAAGATTTAAGGGCTGTCGAGGAGGCATTTGTGCCAGTTATCAAACTGTGTTTTGATG GGATAGAGATTGATATTTTATTTGCAAGATTAGCACTACAGACTATTCCAGAAGATTTGGACTTAAGAGA TGACAGTCTACTTAAAAATTTAGACATTAGATGCATAAGAAGCCTTAATGGTTGCCGGGTAACCGATGAA ATTTTACATCTAGTGCCAAACATTGACAACTTCAGGCTGACTCTGAGAGCCATCAAACTGTGGGCCAAGT GCCACAATATCTATTCCAATATATTAGGTTTCCTCGGAGGTGTTTCCTGGGCCATGCTAGTAGCAAGAAC TTGTCAGCTTTATCCAAATGCAGTAGCGTCAACTCTTGTACGGAAATTCTTCTTGGTATTTTCAGAATGG GAATGGCCAAACCCAGTGTTACTGAAGGAGCCTGAAGAACGGAATCTTAATTTGCCTGTATGGGACCCAA GAGTAAATCCCAGTGATAGGTACCATCTTATGCCTATCATCACACCAGCATACCCACAGCAGAACTCCAC ATACAACGTGTCTATTTCAACCAGGATGGTCATGATTGAGGAGTTTAAACAGGGGCTTGCTATCACACAC GAGATTTTGCTAAGTAAGGCAGAGTGGTCCAAACTCTTTGAAGCTCCAAGCTTCTTTCAAAAGTACAAGC ATTATATTGTACTTCTGGCAAGTGCATCAACAGAAAAACAACATTTAGAATGGGTGGGCTTGGTGGAATC AAAGATCCGAATCCTGGTTGGGAGCTTGGAGAAGAATGAATTTATTACACTGGCACATGTGAATCCACAG TCATTTCCAGCACCCAAAGAAAATCCTGATATGGAAGAATTTCGTACAATGTGGGTGATTGGGTTAGGGC TAAAAAAGCCAGATAATTCTGAAATTCTCAGCATTGATCTCACCTATGATATCCAGTCTTTCACAGATAC TGTTTATAGGCAAGCAGTGAATAGTAAGATGTTTGAGATGGGTATGAAAATTACTGCAATGCATTTAAGA AGAAAGGAACTTCACCAGCTGCTGCCTCATCATGTGCTTCAGGACAAGAAAGCACACTCAACAGAAGGTA GAAGATTGACAGATTTGAACGACAGCAGCTTTGACTTGTCTGCAGGCTGTGAAAACAGCATGTCTGTGCC TTCATCTACTAGCACTATGAAGACAGGCCCATTGATTAGCAGTTCTCAGGGTAGAAACAGTCCTGCCTTG GCTGTAATGACAGCATCTGTGGCTAACATACAGGCTACTGAATTTTCCTTGCAACAGGTGAATACCAATG AAAGTTCAGGGGTTGCATTAAACGAAAGTATTCCTCACGCTGTCTCTCAGCCTGCCATTTCTCCATCACC AAAGGCCATGGTCGCCAGAGTTGTTTCTTCAACATGTCTCATAAGCCATCCAGACCTTCAGGAAACTCAG CAACAAACATATCTAATCCTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205991 representing NM_020144
Red=Cloning site Green=Tags(s) MMPFPVTTQGPPQPAPPPNRYGVSSPISLAVPKETDCLLTQRLIETLRPFGVFEEEEELQRRILVLEKLN NLVKEWIREISESKSLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPSHVDRSDFFTSFYAKLKL QEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDE ILHLVPNIDNFRLTLRAIKLWAKCHNIYSNILGFLGGVSWAMLVARTCQLYPNAVASTLVRKFFLVFSEW EWPNPVLLKEPEERNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSISTRMVMIEEFKQGLAITH EILLSKAEWSKLFEAPSFFQKYKHYIVLLASASTEKQHLEWVGLVESKIRILVGSLEKNEFITLAHVNPQ SFPAPKENPDMEEFRTMWVIGLGLKKPDNSEILSIDLTYDIQSFTDTVYRQAVNSKMFEMGMKITAMHLR RKELHQLLPHHVLQDKKAHSTEGRRLTDLNDSSFDLSAGCENSMSVPSSTSTMKTGPLISSSQGRNSPAL AVMTASVANIQATEFSLQQVNTNESSGVALNESIPHAVSQPAISPSPKAMVARVVSSTCLISHPDLQETQ QQTYLIL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020144 |
| ORF Size | 1911 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020144.5 |
| RefSeq Size | 4271 bp |
| RefSeq ORF | 1914 bp |
| Locus ID | 56903 |
| UniProt ID | Q9NRJ5 |
| Protein Pathways | RNA degradation |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205991 | PAPOLB (Myc-DDK-tagged)-Human poly(A) polymerase beta (testis specific) (PAPOLB) |
CNY 3,990.00 |
|
| RC205991L3 | Lenti-ORF clone of PAPOLB (Myc-DDK-tagged)-Human poly(A) polymerase beta (testis specific) (PAPOLB) |
CNY 5,890.00 |
|
| RC205991L4 | Lenti-ORF clone of PAPOLB (mGFP-tagged)-Human poly(A) polymerase beta (testis specific) (PAPOLB) |
CNY 5,890.00 |
|
| SC304710 | PAPOLB (untagged)-Human poly(A) polymerase beta (testis specific) (PAPOLB) |
CNY 7,220.00 |
