GLO1 (NM_006708) Human Tagged ORF Clone
CAT#: RG203826
- TrueORF®
GLO1 (tGFP-tagged) - Human glyoxalase I (GLO1)
ORF Plasmid: DDK
"NM_006708" in other vectors (6)
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CNY 4,000.00
CNY 4,370.00
Cited in 2 publications. |
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | GLOD1; GLYI; HEL-S-74 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG203826 representing NM_006708
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAACCGCAGCCCCCGTCCGGCGGCCTCACGGACGAGGCCGCCCTCAGTTACTGCTCCGACGCGG ACCCCAGTACCAAGGATTTTCTATTGCAGCAGACCATGCTACGAGTGAAGGATCCTAAGAAGTCACTGGA TTTTTATACTAGAGTTCTTGGAATGACGCTAATCCAAAAATGTGATTTTCCCATTATGAAGTTTTCACTC TACTTCTTGGCTTATGAGGATAAAAATGACATCCCTAAAGAAAAAGATGAAAAAATAGCCTGGGCGCTCT CCAGAAAAGCTACACTTGAGCTGACACACAATTGGGGCACTGAAGATGATGAGACCCAGAGTTACCACAA TGGCAATTCAGACCCTCGAGGATTCGGTCATATTGGAATTGCTGTTCCTGATGTATACAGTGCTTGTAAA AGGTTTGAAGAACTGGGAGTCAAATTTGTGAAGAAACCTGATGATGGTAAAATGAAAGGCCTGGCATTTA TTCAAGATCCTGATGGCTACTGGATTGAAATTTTGAATCCTAACAAAATGGCAACCTTAATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203826 representing NM_006708
Red=Cloning site Green=Tags(s) MAEPQPPSGGLTDEAALSYCSDADPSTKDFLLQQTMLRVKDPKKSLDFYTRVLGMTLIQKCDFPIMKFSL YFLAYEDKNDIPKEKDEKIAWALSRKATLELTHNWGTEDDETQSYHNGNSDPRGFGHIGIAVPDVYSACK RFEELGVKFVKKPDDGKMKGLAFIQDPDGYWIEILNPNKMATLM TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_006708 |
ORF Size | 552 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006708.1, NP_006699.1 |
RefSeq Size | 1993 bp |
RefSeq ORF | 555 bp |
Locus ID | 2739 |
UniProt ID | Q04760 |
Domains | Glyoxalase |
Protein Pathways | Pyruvate metabolism |
Gene Summary | The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Glyoxalase 2 Is Involved in Human Prostate Cancer Progression as Part of a Mechanism Driven By PTEN/PI3K/AKT/mTOR Signaling With Involvement of PKM2 and ERa
,Talesa, VN;Ferri, I;Bellezza, G;Love, HD;Sidoni, A;Antognelli, C;,
Prostate
,PubMed ID 27696457
[Glo1]
|
Methylglyoxal-Induced Endothelial Cell Loss and Inflammation Contribute to the Development of Diabetic Cardiomyopathy
,Vulesevic, B;McNeill, B;Giacco, F;Maeda, K;Blackburn, NJ;Brownlee, M;Milne, RW;Suuronen, EJ;,
Diabetes
,PubMed ID 26956489
[GLO1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203826 | GLO1 (Myc-DDK-tagged)-Human glyoxalase I (GLO1) |
CNY 2,400.00 |
|
RC203826L1 | Lenti ORF clone of Human glyoxalase I (GLO1), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC203826L2 | Lenti ORF clone of Human glyoxalase I (GLO1), mGFP tagged |
CNY 5,890.00 |
|
RC203826L3 | Lenti ORF clone of Human glyoxalase I (GLO1), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC203826L4 | Lenti ORF clone of Human glyoxalase I (GLO1), mGFP tagged |
CNY 4,800.00 |
|
SC115924 | GLO1 (untagged)-Human glyoxalase I (GLO1) |
CNY 2,400.00 |