GLO1 (NM_006708) Human Untagged Clone
CAT#: SC115924
GLO1 (untagged)-Human glyoxalase I (GLO1)
CNY 2,400.00
CNY 2,950.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | GLOD1; GLYI; HEL-S-74 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC115924 sequence for NM_006708 edited (data generated by NextGen Sequencing)
ATGGCAGAACCGCAGCCCCCGTCCGGCGGCCTCACGGACGAGGCCGCCCTCAGTTGCTGC TCCGACGCGGACCCCAGTACCAAGGATTTTCTATTGCAGCAGACCATGCTACGAGTGAAG GATCCTAAGAAGTCACTGGATTTTTATACTAGAGTTCTTGGAATGACGCTAATCCAAAAA TGTGATTTTCCCATTATGAAGTTTTCACTCTACTTCTTGGCTTATGAGGATAAAAATGAC ATCCCTAAAGAAAAAGATGAAAAAATAGCCTGGGCGCTCTCCAGAAAAGCTACACTTGAG CTGACACACAATTGGGGCACTGAAGATGATGAGACCCAGAGTTACCACAATGGCAATTCA GACCCTCGAGGATTCGGTCATATTGGAATTGCTGTTCCTGATGTATACAGTGCTTGTAAA AGGTTTGAAGAACTGGGAGTCAAATTTGTGAAGAAACCTGATGATGGTAAAATGAAAGGC CTGGCATTTATTCAAGATCCTGATGGCTACTGGATTGAAATTTTGAATCCTAACAAAATG GCAACCTTAATGTAG Clone variation with respect to NM_006708.2 >OriGene 5' read for NM_006708 unedited
GTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGGTCGTCTGTGATACTGC AGCGCAGCCATGGCAGAACCGCAGCCCCCGTCCGGCGGCCTCACGGACGAGGCCGCCCTC AGTTGCTGCTCCGACGCGGACCCCAGTACCAAGGATTTTCTATTGCAGCAGACCATGCTA CGAGTGAAGGATCCTAAGAAGTCACTGGATTTTTATACTAGAGTTCTTGGAATGACGCTA ATCCAAAAATGTGATTTTCCCATTATGAAGTTTTCACTCTACTTCTTGGCTTATGAGGAT AAAAATGACATCCCTAAAGAAAAAGATGAAAAAATAGCCTGGGCGCTCTCCAGAAAAGCT ACACTTGAGCTGACACACAATTGGGGCACTGAAGATGATGAGACCCAGAGTTACCACAAT GGCAATTCAGACCCTCGAGGATTCGGTCATATTGGAATTGCTGTTCCTGATGTATACAGT GCTTGTAAAAGGTTTGAAGAACTGGGAGTCAAATTTGTGAAGAAACCTGATGATGGTAAA ATGAAAGGCCTGGCATTTATTCAAGATCCTGATGGCTACTGGATTGAAATTNTGAATCCT AACAAAATGGCAACCTTAATGTAGTGCTGTGAGAATTCTCCTTTGAGATTTCAGAAGAAA GGAAACAATGTGATTCAAGATATTTACATACCAGAAGCATCTAGGACTGATGGATCACTG TCCCCGATCNATTATTCTTCAGTCCATTTCCCCTTCCTATNTCAGCTGTTCCTTTTCACC TAACTGTTCAGTCATTCTGGTTTTCAAGCAGTGCTTTATCTCATGTCCTGAATATAGNTG NNGTACTTTATTTTTTAGTAATAATANAACAGTTCCCTTCAGAGCTGCATTTGCCTCTTC TGCCACTAAATATTNACTCCCTTCAATCTGCT >OriGene 3' read for NM_006708 unedited
GGTTTTTCAGGGGNNCTNTNATNTATTCCAGTTCTCTCAAGCTATTGGCTATCTTGTCTT AAAGCACTCACATAAATCCATTTCACCCAAAAAGGAAACATAAAGTGCTTCTAGCAGTAC AAGCACGGTTGGCATGGCCTTTCCAAAGGTCTTCCACTAGAGTCTAGAGAAATCTAAATA TAGTCATCCACAAACTGGATGTTTTTATTTTCTGAGCCATTAGAGATTTTCAAAATCACT TTGATTTTTAAAAACTCATCAAATGTGAATCATGGCGGGGAAGACCACTGAGCTGATTTC TGATAACTAAGTTATCACTGAACATAATTTATCATATATGGCTACTGGCATCATGAAGAC CTTGGGATAGGGAAGACTCTTTATGAGAAATATAAACATCACTTGTGTAGGAATCACCAG GTGTCCCTAGAGCAGTTTTGTACTAAAGACTTCTAGTGTTTACTNCCTCACCACGGGTAC CTTCCCAACCNNNNNNNNNNNCNCCCCNNCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC NCCCCCCCCCCCCCCCCCCCCNCCCCCCCCCCCCCCCCCCCCCCCCCCCCCNCCCCCCCC CCCNCCCCCNNNCCCCCCCCCCCCNCCNCCCCCCCCCCCCCCCCCCCCCCCCCCCCNCCC CCACCCCCNCCNACCCCCCCCCCCCCCCCCNCCCCCCNCCCCCCCCCCCCCCCCNCNCCC CCCCCCCCNCCCCCCCCCCCCNCCCCCCNCCAACACACCCCCCCCCCCCCCACCCCCCCC CCCACCCCCCCCCCCACCCACCCCCCACACAACCCCCACCCCCACCCACCCCACCCCCCA CACCCCCCCCACCCCCCCACACCACCCNCCCCCCCACNCCACCACCCCCCCACCACACC |
Restriction Sites | NotI-NotI |
ACCN | NM_006708 |
Insert Size | 2100 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_006708.1, NP_006699.1 |
RefSeq Size | 1993 bp |
RefSeq ORF | 555 bp |
Locus ID | 2739 |
UniProt ID | Q04760 |
Domains | Glyoxalase |
Protein Pathways | Pyruvate metabolism |
Gene Summary | The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Peroxynitrite-mediated glyoxalase I epigenetic inhibition drives apoptosis in airway epithelial cells exposed to crystalline silica via a novel mechanism involving argpyrimidine-modified Hsp70, JNK, and NF-?B
,Antognelli, C;Gambelunghe, A;Muzi, G;Talesa, VN;,
Free Radic. Biol. Med.
,PubMed ID 25841781
[GLO1]
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