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Serine Palmitoyltransferase (SPTLC2) (NM_004863) Human Tagged ORF Clone
CAT＃: RG202420
- TrueORF®
SPTLC2 (tGFP-tagged) - Human serine palmitoyltransferase, long chain base subunit 2 (SPTLC2)
ORF Plasmid: DDK
"NM_004863" in other vectors (6)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG202420 representing NM_004863
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCCGGAGCCCGGAGGCTGCTGCTGCCGCCGCACGGTGCGGGCGAATGGCTGCGTGGCGAACGGGG AAGTACGGAACGGGTACGTGAGGAGCAGCGCTGCAGCCGCAGCCGCAGCCGCCGCCGGCCAGATCCATCA TGTTACACAAAATGGAGGACTATATAAAAGACCGTTTAATGAAGCTTTTGAAGAAACACCAATGCTGGTT GCTGTGCTCACGTATGTGGGGTATGGCGTACTCACCCTCTTTGGATATCTTCGAGATTTCTTGAGGTATT GGAGAATTGAAAAGTGTCACCATGCAACAGAAAGAGAAGAACAAAAGGACTTTGTGTCATTGTATCAAGA TTTTGAAAACTTTTATACAAGGAATCTGTACATGAGGATAAGAGACAACTGGAATCGGCCAATCTGTAGT GTGCCTGGAGCCAGGGTGGACATCATGGAGAGACAGTCTCATGATTATAACTGGTCCTTCAAGTATACAG GGAATATAATAAAGGGTGTTATAAACATGGGTTCCTACAACTATCTTGGATTTGCACGGAATACTGGATC ATGTCAAGAAGCAGCCGCCAAAGTCCTTGAGGAGTATGGAGCTGGAGTGTGCAGTACTCGGCAGGAAATT GGAAACCTGGACAAGCATGAAGAACTAGAGGAGCTTGTAGCAAGGTTCTTAGGAGTAGAAGCTGCTATGG CGTATGGCATGGGATTTGCAACGAATTCAATGAACATTCCTGCTCTTGTTGGCAAAGGTTGCCTGATTCT GAGTGATGAACTGAACCATGCATCACTGGTTCTGGGAGCCAGACTGTCAGGAGCAACCATTAGAATCTTC AAACACAACAATATGCAAAGCCTAGAGAAGCTATTGAAAGATGCCATTGTTTATGGTCAGCCTCGGACAC GAAGGCCCTGGAAGAAAATTCTCATCCTTGTGGAAGGAATATATAGCATGGAGGGATCTATTGTTCGTCT TCCTGAAGTGATTGCCCTCAAGAAGAAATACAAGGCATACTTGTATCTGGATGAGGCTCACAGCATTGGC GCCCTGGGCCCCACAGGCCGGGGTGTGGTGGAGTACTTTGGCCTGGATCCCGAGGATGTGGATGTTATGA TGGGAACGTTCACAAAGAGTTTTGGTGCTTCTGGAGGATATATTGGAGGCAAGAAGGAGCTGATAGACTA CCTGCGAACACATTCTCATAGTGCAGTGTATGCCACGTCATTGTCACCTCCTGTAGTGGAGCAGATCATC ACCTCCATGAAGTGCATCATGGGGCAGGATGGCACCAGCCTTGGTAAAGAGTGTGTACAACAGTTAGCTG AAAACACCAGGTATTTCAGGAGACGCCTGAAAGAGATGGGCTTCATCATCTATGGAAATGAAGACTCTCC AGTAGTGCCTTTGATGCTCTACATGCCTGCCAAAATTGGCGCCTTTGGACGGGAGATGCTGAAGCGGAAC ATCGGTGTCGTTGTGGTTGGATTTCCTGCCACCCCAATTATTGAGTCCAGAGCCAGGTTTTGCCTGTCAG CAGCTCATACCAAAGAAATACTTGATACTGCTTTAAAGGAGATAGATGAAGTTGGGGACCTATTGCAGCT GAAGTATTCCCGTCATCGGTTGGTACCTCTACTGGACAGGCCCTTTGACGAGACGACGTATGAAGAAACA GAAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG202420 representing NM_004863
Red=Cloning site Green=Tags(s) MRPEPGGCCCRRTVRANGCVANGEVRNGYVRSSAAAAAAAAAGQIHHVTQNGGLYKRPFNEAFEETPMLV AVLTYVGYGVLTLFGYLRDFLRYWRIEKCHHATEREEQKDFVSLYQDFENFYTRNLYMRIRDNWNRPICS VPGARVDIMERQSHDYNWSFKYTGNIIKGVINMGSYNYLGFARNTGSCQEAAAKVLEEYGAGVCSTRQEI GNLDKHEELEELVARFLGVEAAMAYGMGFATNSMNIPALVGKGCLILSDELNHASLVLGARLSGATIRIF KHNNMQSLEKLLKDAIVYGQPRTRRPWKKILILVEGIYSMEGSIVRLPEVIALKKKYKAYLYLDEAHSIG ALGPTGRGVVEYFGLDPEDVDVMMGTFTKSFGASGGYIGGKKELIDYLRTHSHSAVYATSLSPPVVEQII TSMKCIMGQDGTSLGKECVQQLAENTRYFRRRLKEMGFIIYGNEDSPVVPLMLYMPAKIGAFGREMLKRN IGVVVVGFPATPIIESRARFCLSAAHTKEILDTALKEIDEVGDLLQLKYSRHRLVPLLDRPFDETTYEET ED TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004863 |
| ORF Size | 1686 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004863.1 |
| RefSeq Size | 7250 bp |
| RefSeq ORF | 1689 bp |
| Locus ID | 9517 |
| UniProt ID | O15270 |
| Domains | aminotran_1_2 |
| Protein Pathways | Metabolic pathways, Sphingolipid metabolism |
| Gene Summary | This gene encodes a long chain base subunit of serine palmitoyltransferase. Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine. Mutations in this gene were identified in patients with hereditary sensory neuropathy type I. [provided by RefSeq, Mar 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202420 | SPTLC2 (Myc-DDK-tagged)-Human serine palmitoyltransferase, long chain base subunit 2 (SPTLC2) |
CNY 4,184.00 |
|
| RC202420L1 | Lenti ORF clone of Human serine palmitoyltransferase, long chain base subunit 2 (SPTLC2), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC202420L2 | Lenti ORF clone of Human serine palmitoyltransferase, long chain base subunit 2 (SPTLC2), mGFP tagged |
CNY 6,460.00 |
|
| RC202420L3 | Lenti ORF clone of Human serine palmitoyltransferase, long chain base subunit 2 (SPTLC2), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC202420L4 | Lenti ORF clone of Human serine palmitoyltransferase, long chain base subunit 2 (SPTLC2), mGFP tagged |
CNY 6,460.00 |
|
| SC127529 | SPTLC2 (untagged)-Human serine palmitoyltransferase, long chain base subunit 2 (SPTLC2) |
CNY 4,192.00 |
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