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ATP dependent metalloprotease YME1L1 (YME1L1) (NM_014263) Human Tagged ORF Clone
CAT＃: RG201088
- TrueORF®
YME1L1 (tGFP-tagged) - Human YME1-like 1 (S. cerevisiae) (YME1L1), nuclear gene encoding mitochondrial protein, transcript variant 3
ORF Plasmid: DDK
"NM_014263" in other vectors (6)
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CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | FTSH; MEG4; OPA11; PAMP; YME1L |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG201088 representing NM_014263
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTTCCTTGTCGAGCACGGTGCAACCCCAGGTTACAGTTCCTCTGAGTCATCTCATCAATGCCTTCC ATACACCAAAAAACACTTCTGTTTCTCTCAGTGGAGTGTCAGTTTCTCAAAACCAGCATCGAGATGTAGT TCCTGAGCATGAGGCTCCCAGCAGTGAGCCTTCACTTAACTTAAGGGACCTTGGATTATCTGAACTAAAA ATTGGACAGATTGATCAGCTGGTAGAAAATCTACTTCCTGGATTTTGTAAAGGCAAAAACATTTCTTCCC ATTGGCATACATCCCATGTCTCTGCACAATCCTTCTTTGAAAATAAATATGGTAACTTAGATATATTTAG TACATTACGTTCCTCTTGCTTGTATCGACATCATTCAAGAGCTCTTCAAAGCATTTGTTCAGATCTTCAG TACTGGCCAGTTTTCATACAGTCTCGGGGTTTTAAAACTTTGAAATCAAGGACACGACGTCTCCAGTCTA CCTCCGAGAGATTAGCTGAAACACAGAATATAGCGCCATCATTCGTGAAGGGGTTTCTTTTGCGGGACAG AGGATCAGATGTTGAGAGTTTGGACAAACTCATGAAAACCAAAAATATACCTGAAGCTCACCAAGATGCA TTTAAAACTGGTTTTGCGGAAGGTTTTCTGAAAGCTCAAGCACTCACACAAAAAACCAATGATTCCCTAA GGCGAACCCGTCTGATTCTCTTCGTTCTGCTGCTATTCGGCATTTATGGACTTCTAAAAAACCCATTTTT ATCTGTCCGCTTCCGGACAACAACAGGGCTTGATTCTGCAGTAGATCCTGTCCAGATGAAAAATGTCACC TTTGAACATGTTAAAGGGGTGGAGGAAGCTAAACAAGAATTACAGGAAGTTGTTGAATTCTTGAAAAATC CACAAAAATTTACTATTCTTGGAGGTAAACTTCCAAAAGGAATTCTTTTAGTTGGACCCCCAGGGACTGG AAAGACACTTCTTGCCCGAGCTGTGGCGGGAGAAGCTGATGTTCCTTTTTATTATGCTTCTGGATCCGAA TTTGATGAGATGTTTGTGGGTGTGGGAGCCAGCCGTATCAGAAATCTTTTTAGGGAAGCAAAGGCGAATG CTCCTTGTGTTATATTTATTGATGAATTAGATTCTGTTGGTGGGAAGAGAATTGAATCTCCAATGCATCC ATATTCAAGGCAGACCATAAATCAACTTCTTGCTGAAATGGATGGTTTTAAACCCAATGAAGGAGTTATC ATAATAGGAGCCACAAACTTCCCAGAGGCATTAGATAATGCCTTAATACGTCCTGGTCGTTTTGACATGC AAGTTACAGTTCCAAGGCCAGATGTAAAAGGTCGAACAGAAATTTTGAAATGGTATCTCAATAAAATAAA GTTTGATCAATCCGTTGATCCAGAAATTATAGCTCGAGGTACTGTTGGCTTTTCCGGAGCAGAGTTGGAG AATCTTGTGAACCAGGCTGCATTAAAAGCAGCTGTTGATGGAAAAGAAATGGTTACCATGAAGGAGCTGG AGTTTTCCAAAGACAAAATTCTAATGGGGCCTGAAAGAAGAAGTGTGGAAATTGATAACAAAAACAAAAC CATCACAGCATATCATGAATCTGGTCATGCCATTATTGCATATTACACAAAAGATGCAATGCCTATCAAC AAAGCTACAATCATGCCACGGGGGCCAACACTTGGACATGTGTCCCTGTTACCTGAGAATGACAGATGGA ATGAAACTAGAGCCCAGCTGCTTGCACAAATGGATGTTAGTATGGGAGGAAGAGTGGCAGAGGAGCTTAT ATTTGGAACCGACCATATTACAACAGGTGCTTCCAGTGATTTTGATAATGCCACTAAAATAGCAAAGCGG ATGGTTACCAAATTTGGAATGAGTGAAAAGCTTGGAGTTATGACCTACAGTGATACAGGGAAACTAAGTC CAGAAACCCAATCTGCCATCGAACAAGAAATAAGAATCCTTCTAAGGGACTCATATGAACGAGCAAAACA TATCTTGAAAACTCATGCAAAGGAGCATAAGAATCTCGCAGAAGCTTTATTGACCTATGAGACTTTGGAT GCCAAAGAGATTCAAATTGTTCTTGAGGGGAAAAAGTTGGAAGTGAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201088 representing NM_014263
Red=Cloning site Green=Tags(s) MFSLSSTVQPQVTVPLSHLINAFHTPKNTSVSLSGVSVSQNQHRDVVPEHEAPSSEPSLNLRDLGLSELK IGQIDQLVENLLPGFCKGKNISSHWHTSHVSAQSFFENKYGNLDIFSTLRSSCLYRHHSRALQSICSDLQ YWPVFIQSRGFKTLKSRTRRLQSTSERLAETQNIAPSFVKGFLLRDRGSDVESLDKLMKTKNIPEAHQDA FKTGFAEGFLKAQALTQKTNDSLRRTRLILFVLLLFGIYGLLKNPFLSVRFRTTTGLDSAVDPVQMKNVT FEHVKGVEEAKQELQEVVEFLKNPQKFTILGGKLPKGILLVGPPGTGKTLLARAVAGEADVPFYYASGSE FDEMFVGVGASRIRNLFREAKANAPCVIFIDELDSVGGKRIESPMHPYSRQTINQLLAEMDGFKPNEGVI IIGATNFPEALDNALIRPGRFDMQVTVPRPDVKGRTEILKWYLNKIKFDQSVDPEIIARGTVGFSGAELE NLVNQAALKAAVDGKEMVTMKELEFSKDKILMGPERRSVEIDNKNKTITAYHESGHAIIAYYTKDAMPIN KATIMPRGPTLGHVSLLPENDRWNETRAQLLAQMDVSMGGRVAEELIFGTDHITTGASSDFDNATKIAKR MVTKFGMSEKLGVMTYSDTGKLSPETQSAIEQEIRILLRDSYERAKHILKTHAKEHKNLAEALLTYETLD AKEIQIVLEGKKLEVR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014263 |
| ORF Size | 2148 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014263.4 |
| RefSeq Size | 3865 bp |
| RefSeq ORF | 2151 bp |
| Locus ID | 10730 |
| UniProt ID | Q96TA2 |
| Domains | Peptidase_M41, AAA, AAA |
| Protein Families | Druggable Genome, Protease |
| Gene Summary | The protein encoded by this gene is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201088 | YME1L1 (Myc-DDK-tagged)-Human YME1-like 1 (S. cerevisiae) (YME1L1), nuclear gene encoding mitochondrial protein, transcript variant 3 |
CNY 3,990.00 |
|
| RC201088L1 | Lenti ORF clone of Human YME1-like 1 (S. cerevisiae) (YME1L1), nuclear gene encoding mitochondrial protein, transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201088L2 | Lenti ORF clone of Human YME1-like 1 (S. cerevisiae) (YME1L1), nuclear gene encoding mitochondrial protein, transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RC201088L3 | Lenti ORF clone of Human YME1-like 1 (S. cerevisiae) (YME1L1), nuclear gene encoding mitochondrial protein, transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201088L4 | Lenti ORF clone of Human YME1-like 1 (S. cerevisiae) (YME1L1), nuclear gene encoding mitochondrial protein, transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| SC110195 | YME1L1 (untagged)-Human YME1-like 1 (S. cerevisiae) (YME1L1), nuclear gene encoding mitochondrial protein, transcript variant 3 |
CNY 2,950.00 |
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