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Eph receptor B3 (EPHB3) (NM_004443) Human Tagged ORF Clone
CAT＃: RC600050
- TrueORF®
EPHB3 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens EPH receptor B3, Signal peptide (1-33) plus EC domain (34-559)
"NM_004443" in other vectors (7)
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CNY 4,160.00
CNY 6,370.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | DDK-His |
| Synonyms | EK2; ETK2; HEK2; TYRO6 |
| Vector | pCMV6-XL5-DDK-His |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>RC600050 representing leader sequence plus the extracellular domain region of NM_004443
Red=Cloning site Blue=ORF Green=Tags(s) GTAATACGACTCACTATAGGGCGGCCGCGAATTCGTCGACTGGATCTGGTACCGAGGAGATCCGCCGCCG CGATCGCC ATGGCCAGAGCCCGCCCGCCGCCGCCGCCGTCGCCGCCGCCGGGGCTTCTGCCGCTGCTCCCTCCGCTGC TGCTGCTGCCGCTGCTGCTGCTGCCCGCCGGCTGCCGGGCGCTGGAAGAGACCCTCATGGACACAAAATG GGTAACATCTGAGTTGGCGTGGACATCTCATCCAGAAAGTGGGTGGGAAGAGGTGAGTGGCTACGATGAG GCCATGAATCCCATCCGCACATACCAGGTGTGTAATGTGCGCGAGTCAAGCCAGAACAACTGGCTTCGCA CGGGGTTCATCTGGCGGCGGGATGTGCAGCGGGTCTACGTGGAGCTCAAGTTCACTGTGCGTGACTGCAA CAGCATCCCCAACATCCCCGGCTCCTGCAAGGAGACCTTCAACCTCTTCTACTACGAGGCTGACAGCGAT GTGGCCTCAGCCTCCTCCCCCTTCTGGATGGAGAACCCCTACGTGAAAGTGGACACCATTGCACCCGATG AGAGCTTCTCGCGGCTGGATGCCGGCCGTGTCAACACCAAGGTGCGCAGCTTTGGGCCACTTTCCAAGGC TGGCTTCTACCTGGCCTTCCAGGACCAGGGCGCCTGCATGTCGCTCATCTCCGTGCGCGCCTTCTACAAG AAGTGTGCATCCACCACCGCAGGCTTCGCACTCTTCCCCGAGACCCTCACTGGGGCGGAGCCCACCTCGC TGGTCATTGCTCCTGGCACCTGCATCCCTAACGCCGTGGAGGTGTCGGTGCCACTCAAGCTCTACTGCAA CGGCGATGGGGAGTGGATGGTGCCTGTGGGTGCCTGCACCTGTGCCACCGGCCATGAGCCAGCTGCCAAG GAGTCCCAGTGCCGCCCCTGTCCCCCTGGGAGCTACAAGGCGAAGCAGGGAGAGGGGCCCTGCCTCCCAT GTCCCCCCAACAGCCGTACCACCTCCCCAGCCGCCAGCATCTGCACCTGCCACAATAACTTCTACCGTGC AGACTCGGACTCTGCGGACAGTGCCTGTACCACCGTGCCATCTCCACCCCGAGGTGTGATCTCCAATGTG AATGAAACCTCACTGATCCTCGAGTGGAGTGAGCCCCGGGACCTGGGTGGCCGGGATGACCTCCTGTACA ATGTCATCTGCAAGAAGTGCCATGGGGCTGGAGGGGCCTCAGCCTGCTCACGCTGTGATGACAACGTGGA GTTTGTGCCTCGGCAGCTGGGCCTGACGGAGCGCCGGGTCCACATCAGCCATCTGCTGGCCCACACGCGC TACACCTTTGAGGTGCAGGCGGTCAACGGTGTCTCGGGCAAGAGCCCTCTGCCGCCTCGTTATGCGGCCG TGAATATCACCACAAACCAGGCTGCCCCGTCTGAAGTGCCCACACTACGCCTGCACAGCAGCTCAGGCAG CAGCCTCACCCTATCCTGGGCACCCCCAGAGCGGCCCAACGGAGTCATCCTGGACTACGAGATGAAGTAC TTTGAGAAGAGCGAGGGCATCGCCTCCACAGTGACCAGCCAGATGAACTCCGTGCAGCTGGACGGGCTTC GGCCTGACGCCCGCTATGTGGTCCAGGTCCGTGCCCGCACAGTAGCTGGCTATGGGCAGTACAGCCGCCC TGCCGAGTTTGAGACCACAAGTGAGAGAGGCTCTGGGGCCCAGCAGCTCCAGGAGCAGCTTCCCCTC ACGCGTTCAGGCGACTACAAGGATGACGACGATAAGGGATCTCATCATCACCATCACCATTAATGAGATC TGGTACCGATATCAAGCTTGTCGACTCTAGA >RC600050 representing signal peptide plus the extracellular domain region of NM_004443
Red=Cloning sites Green= DDK and 6XHIS Tags MARARPPPPPSPPPGLLPLLPPLLLLPLLLLPAGCRALEETLMDTKWVTSELAWTSHPESGWEEVSGYDE AMNPIRTYQVCNVRESSQNNWLRTGFIWRRDVQRVYVELKFTVRDCNSIPNIPGSCKETFNLFYYEADSD VASASSPFWMENPYVKVDTIAPDESFSRLDAGRVNTKVRSFGPLSKAGFYLAFQDQGACMSLISVRAFYK KCASTTAGFALFPETLTGAEPTSLVIAPGTCIPNAVEVSVPLKLYCNGDGEWMVPVGACTCATGHEPAAK ESQCRPCPPGSYKAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRADSDSADSACTTVPSPPRGVISNV NETSLILEWSEPRDLGGRDDLLYNVICKKCHGAGGASACSRCDDNVEFVPRQLGLTERRVHISHLLAHTR YTFEVQAVNGVSGKSPLPPRYAAVNITTNQAAPSEVPTLRLHSSSGSSLTLSWAPPERPNGVILDYEMKY FEKSEGIASTVTSQMNSVQLDGLRPDARYVVQVRARTVAGYGQYSRPAEFETTSERGSGAQQLQEQLPL TRSGTRSGDYKDDDDKGSHHHHHH |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NM_004443 |
| ORF Size | 1677 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the extra cellular domain of the protein with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004443.3, NP_004434.2 |
| RefSeq Size | 4234 bp |
| RefSeq ORF | 2997 bp |
| Locus ID | 2049 |
| UniProt ID | P54753 |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Protein Pathways | Axon guidance |
| MW | 60.9 kDa |
| Gene Summary | Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. This gene encodes a receptor for ephrin-B family members. [provided by RefSeq, Mar 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208364 | EPHB3 (Myc-DDK-tagged)-Human EPH receptor B3 (EPHB3) |
CNY 10,960.00 |
|
| RC208364L1 | Lenti ORF clone of Human EPH receptor B3 (EPHB3), Myc-DDK-tagged |
CNY 13,360.00 |
|
| RC208364L2 | Lenti ORF clone of Human EPH receptor B3 (EPHB3), mGFP tagged |
CNY 13,360.00 |
|
| RC208364L3 | Lenti ORF clone of Human EPH receptor B3 (EPHB3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208364L4 | Lenti ORF clone of Human EPH receptor B3 (EPHB3), mGFP tagged |
CNY 13,360.00 |
|
| RG208364 | EPHB3 (tGFP-tagged) - Human EPH receptor B3 (EPHB3) |
CNY 12,560.00 |
|
| SC117356 | EPHB3 (untagged)-Human EPH receptor B3 (EPHB3) |
CNY 10,976.00 |
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