ADCY10 (NM_001297772) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HCA2; HEL-S-7a; hsAC; SAC; SACI; Sacy
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240169 representing NM_001297772 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTCTCTGAAGGTGATGCACTGCTAGCCCTGTGGAGGGTGGAGCGAAAGCAGCTGAAAAACATTA TCACAGTGGTAATTAAATGTAGCCTGGAGATCCATGGATTGTTTGAGACCCAGGAGTGGGAAGAAGGCCT AGACATCCGAGTCAAGATAGGACTGGCTGCTGGCCACATCAGCATGTTGGTCTTTGGAGATGAAACACAC AGCCACTTTCTGGTGATTGGTCAGGCAGTGGACGATGTGCGCCTTGCCCAGAACATGGCTCAGATGAATG ATGTTATTCTGTCACCAAACTGCTGGCAGCTCTGTGACCGGAGCATGATTGAAATTGAGAGTGTTCCAGA TCAGAGAGCAGTTAAGGTTAACTTCTTAAAACCACCCCCCAATTTTAATTTTGATGAATTTTTCACAAAG TGTACGACCTTCATGCATTATTATCCTTCTGGTGAGCACAAAAACCTCCTGAGGCTTGCATGCACGCTGA AGCCTGATCCTGAACTGGAGATGTCCCTACAAAAGTATGTGATGGAAAGCATTTTGAAGCAGATTGATAA CAAACAGCTTCAGGGCTATTTATCTGAGCTTCGCCCAGTGACGATTGTGTTTGTGAACCTGATGTTTGAA GACCAAGACAAAGCAGAAGAGATAGGCCCAGCCATCCAGGATGCCTATATGCACATCACTTCTGTCCTGA AGATCTTCCAAGGCCAAATCAATAAAGTCTTCATGTTTGACAAGGGCTGCTCTTTCCTCTGTGTCTTTGG CTTCCCTGGGGAAAAGGTACCTGACGAGCTCACTCATGCTCTGGAATGTGCTATGGATATATTTGACTTC TGCTCTCAAGTCCACAAAATCCAAACTGTATCCATCGGTGTTGCCAGTGGGATTGTCTTCTGTGGGATCG TTGGACACACTGTGAGACACGAGTACACAGTCATTGGTCAAAAAGTCAACTTAGCTGCCAGGATGATGAT GTACTACCCAGGAATTGTGACCTGCGACTCTGTCACCTACAATGGGAGCAACCTACCAGCGTACTTTTTT AAAGAGCTTCCAAAGAAAGTTATGAAAGGTGTTGCAGATTCTGGACCATTGTATCAGTATTGGGGCCGTA CTGAGAAAGTCATGTTTGGTATGGCGTGCCTCATCTGCAACAGAAAGGAGGATTACCCTTTGCTGGGACG TAATAAAGAGATCAACTACTTCATGTATACTATGAAGAAATTTTTGATATCTAACAGCAGCCAAGTCTTA ATGTATGAGGGATTACCAGGATATGGAAAAAGCCAGATACTTATGAAAATTGAGTACCTGGCCCAAGGTA AGAATCACAGGATTATTGCCATTTCATTGAATAAGATCAGCTTCCATCAAACTTTCTATACCATCCAGAT GTTCATGGCCAATGTCCTAGGCCTAGACACTTGTAAACATTATAAAGAACGACAGACCAACCTTCGAAAT AAAGTCATGACACTGTTGGATGAAAAGTTCTACTGTCTTCTTAATGACATTTTCCATGTTCAGTTCCCTA TTTCTCGGGAGATTTCCAGGATGAGCACCTTGAAAAAGCAAAAACAATTGGAAATATTGTTTATGAAGAT CTTGAAGCTGATAGTGAAAGAGGAAAGGATTATTTTTATCATTGATGAGGCCCAGTTTGTGGATTCGACC TCCTGGAGATTTATGGAGAAGCTTATCCGGACTCTTCCTATCTTCATCATTATGTCCCTGTGTCCCTTCG TTAACATTCCCTGTGCAGCTGCCAGGGCCGTAATAAAGAACAGGAACACCACCTACATTGTCATTGGTGC AGTACAGCCTAACGACATCTCCAACAAGATCTGTCTTGACCTCAATGTGAGCTGCATCTCCAAAGAACTG GACTCGTACCTGGGGGAGGGAAGCTGTGGGATTCCATTTTACTGTGAAGAATTGCTTAAAAACCTGGAAC ATCATGAGGTACTCGTTTTCCAACAAACGGAGTCTGAGGAAAAGACAAATAGGACCTGGAATAACCTGTT CAAGTATTCCATTAAGCTAACAGAGAAGTTAAACATGGTTACTCTCCATAGTGATAAGGAAAGTGAAGAA GTCTGTCACCTCACAAGTGGTGTCAGACTGAAAAACCTGTCACCTCCAACGTCATTAAAAGAAATCTCTC TGATCCAGCTGGATAGCATGAGACTTTCCCACCAAATGCTGGTGAGATGTGCTGCCATCATTGGCCTGAC CTTCACCACTGAGTTGTTGTTTGAGATTCTCCCCTGTTGGAATATGAAGATGATGATCAAGACCCTGGCA ACCCTAGTGGAATCTAACATTTTTTATTGTTTCCGGAATGGCAAGGAGCTTCAAAAGGCCCTGAAACAGA ATGATCCCTCATTTGAGGTGCACTATCGTTCCTTGTCTCTGAAGCCCAGTGAAGGGATGGATCACGGTGA AGAGGAACAGCTTCGTGAACTGGAGAATGAGGTGATCGAGTGCCACAGGATTCGATTCTGTAACCCTATG ATGCAGAAAACAGCCTACGAGCTGTGGCTCAAGGACCAGAGAAAAGCCATGCACTTGAAATGTGCCCGCT TTTTAGAAGAAGATGCCCACAGATGTGACCACTGCCGAGGCAGGGACTTCATTCCCTATCATCACTTCAC AGTGAATATTCGGCTCAACGCTTTAGACATGGATGCCATTAAAAAGATGGCTATGTCTCATGGATTTAAA ACTGAAGAAAAGCTTATCTTGTCCAACTCAGAGATTCCTGAGACATCTGCATTTTTTCCTGAAAATCGCA GTCCTGAAGAAATAAGAGAAAAGATCTTGAATTTCTTTGACCACGTTTTAACAAAAATGAAGACATCTGA CGAAGACATTATCCCTCTGGAATCTTGCCAGTGTGAAGAAATCCTAGAGATTGTCATCTTGCCTCTGGCC CACCATTTTCTGGCTTTGGGAGAAAATGACAAAGCCTTATATTACTTCTTAGAAATTGCATCTGCTTATC TCATCTTTTGTGATAACTACATGGCATACATGTATTTGAATGAAGGACAGAAGTTGCTAAAAACTCTCAA GAAGGACAAATCTTGGAGCCAGACATTTGAGTCTGCCACCTTTTACAGCCTCAAAGGTGAGGTCTGTTTC AATATGGGCCAGATAGTGCTTGCCAAGAAAATGCTGAGGAAGGCACTGAAGCTCCTCAACCGAATCTTTC CTTACAACTTAATCTCCTTGTTTCTCCATATCCATGTCGAGAAAAACAGACACTTTCATTATGTGAATCG GCAGGCCCAAGAGAGCCCACCTCCAGGGAAGAAGAGGCTGGCACAACTTTACCGGCAAACTGTCTGCCTT TCCTTGCTGTGGCGCATCTATAGCTACAGTTATCTTTTTCACTGCAAGTATTATGCCCACCTGGCAGTTA TGATGCAAATGAATACTGCACTGGAAACTCAAAATTGTTTCCAGATCATTAAGGCTTACCTAGACTATTC GCTATACCACCACCTGGCTGGCTACAAAGGTGTGTGGTTCAAATATGAAGTCATGGCCATGGAGCACATC TTCAACCTCCCCCTGAAAGGCGAGGGCATTGAAATCGTGGCATACGTGGCTGAGACACTGGTCTTCAACA AGCTCATAATGGGACACCTGGATTTGGCCATTGAGTTAGGCTCCCGAGCCCTTCAGATGTGGGCACTGCT CCAGAATCCCAACCGACATTATCAGTCCCTCTGCAGACTTAGCAGATGTCTCCTTCTGAACAGCAGATAC CCGCAATTGATCCAGGTGCTGGGGCGGCTGTGGGAGCTTTCTGTAACACAGGAACACATCTTCAGCAAGG CATTTTTCTATTTTGTCTGCTTGGACATCCTGCTTTATTCTGGTTTTGTTTATAGAACATTTGAAGAATG TTTGGAATTCATACACCAATACGAAAACAACAGAATCCTCAAGTTCCACAGTGGACTCCTCCTGGGACTT TATTCCTCTGTAGCTATCTGGTATGCCAGACTTCAGGAATGGGACAACTTTTACAAATTTTCCAATAGAG CTAAAAATCTTTTGCCAAGAAGAACCATGACACTTACTTACTATGACGGAATATCTAGGTACATGGAGGG GCAAGTTCTTCACCTTCAAAAACAAATCAAAGAACAGTCAGAGAATGCCCAAGCCAGTGGGGAGGAGCTA CTCAAGAACTTGGAGAATCTGGTGGCTCAAAATACCACTGGCCCTGTCTTTTGCCCAAGGCTCTACCACC TGATGGCTTACGTCTGTATATTAATGGGAGATGGGCAGAAATGTGGCCTCTTCCTGAACACAGCCTTGCG GCTCTCTGAAACACAGGGGAATATACTGGAGAAATGCTGGCTGAACATGAACAAAGAATCATGGTACTCA ACCTCTGAGTTAAAAGAAGACCAATGGCTTCAGACGATCTTGAGTCTCCCATCATGGGAAAAAATTGTAG CAGGCAGGGTAAACATTCAGGATCTTCAAAAAAACAAATTCCTGATGAGAGCTAATACCGTGGACAATCA TTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240169 representing NM_001297772 Red=Cloning site Green=Tags(s) MSLSEGDALLALWRVERKQLKNIITVVIKCSLEIHGLFETQEWEEGLDIRVKIGLAAGHISMLVFGDETH SHFLVIGQAVDDVRLAQNMAQMNDVILSPNCWQLCDRSMIEIESVPDQRAVKVNFLKPPPNFNFDEFFTK CTTFMHYYPSGEHKNLLRLACTLKPDPELEMSLQKYVMESILKQIDNKQLQGYLSELRPVTIVFVNLMFE DQDKAEEIGPAIQDAYMHITSVLKIFQGQINKVFMFDKGCSFLCVFGFPGEKVPDELTHALECAMDIFDF CSQVHKIQTVSIGVASGIVFCGIVGHTVRHEYTVIGQKVNLAARMMMYYPGIVTCDSVTYNGSNLPAYFF KELPKKVMKGVADSGPLYQYWGRTEKVMFGMACLICNRKEDYPLLGRNKEINYFMYTMKKFLISNSSQVL MYEGLPGYGKSQILMKIEYLAQGKNHRIIAISLNKISFHQTFYTIQMFMANVLGLDTCKHYKERQTNLRN KVMTLLDEKFYCLLNDIFHVQFPISREISRMSTLKKQKQLEILFMKILKLIVKEERIIFIIDEAQFVDST SWRFMEKLIRTLPIFIIMSLCPFVNIPCAAARAVIKNRNTTYIVIGAVQPNDISNKICLDLNVSCISKEL DSYLGEGSCGIPFYCEELLKNLEHHEVLVFQQTESEEKTNRTWNNLFKYSIKLTEKLNMVTLHSDKESEE VCHLTSGVRLKNLSPPTSLKEISLIQLDSMRLSHQMLVRCAAIIGLTFTTELLFEILPCWNMKMMIKTLA TLVESNIFYCFRNGKELQKALKQNDPSFEVHYRSLSLKPSEGMDHGEEEQLRELENEVIECHRIRFCNPM MQKTAYELWLKDQRKAMHLKCARFLEEDAHRCDHCRGRDFIPYHHFTVNIRLNALDMDAIKKMAMSHGFK TEEKLILSNSEIPETSAFFPENRSPEEIREKILNFFDHVLTKMKTSDEDIIPLESCQCEEILEIVILPLA HHFLALGENDKALYYFLEIASAYLIFCDNYMAYMYLNEGQKLLKTLKKDKSWSQTFESATFYSLKGEVCF NMGQIVLAKKMLRKALKLLNRIFPYNLISLFLHIHVEKNRHFHYVNRQAQESPPPGKKRLAQLYRQTVCL SLLWRIYSYSYLFHCKYYAHLAVMMQMNTALETQNCFQIIKAYLDYSLYHHLAGYKGVWFKYEVMAMEHI FNLPLKGEGIEIVAYVAETLVFNKLIMGHLDLAIELGSRALQMWALLQNPNRHYQSLCRLSRCLLLNSRY PQLIQVLGRLWELSVTQEHIFSKAFFYFVCLDILLYSGFVYRTFEECLEFIHQYENNRILKFHSGLLLGL YSSVAIWYARLQEWDNFYKFSNRAKNLLPRRTMTLTYYDGISRYMEGQVLHLQKQIKEQSENAQASGEEL LKNLENLVAQNTTGPVFCPRLYHLMAYVCILMGDGQKCGLFLNTALRLSETQGNILEKCWLNMNKESWYS TSELKEDQWLQTILSLPSWEKIVAGRVNIQDLQKNKFLMRANTVDNHF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001297772
ORF Size	4554 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001297772.2
RefSeq Size	5469 bp
RefSeq ORF	4557 bp
Locus ID	55811
UniProt ID	Q96PN6
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Purine metabolism
MW	177 kDa
Gene Summary	The protein encoded by this gene belongs to a distinct class of adenylyl cyclases that is soluble and insensitive to G protein or forskolin regulation. Activity of this protein is regulated by bicarbonate. Variation at this gene has been observed in patients with absorptive hypercalciuria. Alternatively spliced transcript variants encoding different isoforms have been observed. There is a pseudogene of this gene on chromosome 6. [provided by RefSeq, Jul 2014]

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