AIP1 (MAGI2) (NM_001301128) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ACVRIP1; AIP-1; AIP1; ARIP1; MAGI-2; NPHS15; SSCAM
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240148 representing NM_001301128 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAAAAGCTTGAAAAAGAAAAGCCACTGGACTAGCAAAGTCCATGAGAGTGTCATTGGCAGGAACC CGGAGGGCCAGCTGGGCTTTGAACTGAAGGGGGGCGCCGAGAATGGACAGTTCCCCTACCTGGGGGAGGT GAAGCCCGGCAAGGTGGCCTATGAGAGCGGCAGCAAATTGGTGTCGGAGGAGCTGCTGCTGGAGGTGAAC GAGACCCCCGTGGCGGGGCTCACCATCAGGGACGTGCTGGCCGTGATCAAACACTGCAAGGACCCCCTCC GGCTCAAGTGTGTCAAGCAAGGAGGAATTGTTGATAAAGACCTTCGTCACTACCTCAACTTACGATTTCA AAAGGGTTCTGTGGACCATGAGCTTCAGCAAATCATTCGTGACAACCTCTACCTCCGCACGGTGCCATGC ACCACAAGGCCACATAAGGAGGGTGAGGTCCCTGGAGTGGATTATATTTTCATCACTGTTGAAGATTTTA TGGAATTGGAGAAAAGTGGTGCTCTCCTAGAAAGTGGGACTTATGAAGACAATTACTACGGTACCCCAAA GCCGCCAGCAGAACCAGCACCATTATTGTTAAATGTAACAGACCAGATACTTCCAGGAGCCACTCCAAGT GCTGAAGGAAAACGGAAGAGGAATAAATCAGTGAGCAACATGGAGAAAGCCAGTATAGAGCCTCCTGAGG AGGAAGAGGAAGAGAGGCCTGTGGTCAATGGAAATGGAGTAGTAGTAACACCAGAATCCAGTGAACATGA AGACAAAAGTGCAGGTGCCTCAGGGGAGATGCCCTCCCAGCCTTATCCTGCACCAGTGTACAGTCAGCCT GAGGAGCTGAAGGAGCAGATGGATGACACAAAGCCAACTAAACCTGAAGACAATGAGGAACCAGACCCAT TGCCTGATAACTGGGAAATGGCCTATACAGAGAAGGGCGAAGTCTACTTCATTGACCATAACACAAAGAC AACATCATGGCTGGATCCACGACTTGCGAAAAAGGCTAAACCTCCAGAAGAGTGCAAAGAAAATGAGCTT CCATATGGCTGGGAAAAAATCGATGATCCCATTTATGGCACTTATTATGTTGACCACATAAATAGAAGAA CACAGTTTGAAAATCCTGTCCTGGAAGCAAAAAGGAAGCTACAGCAACATAACATGCCCCACACAGAACT TGGAACAAAGCCCCTGCAGGCCCCAGGTTTCCGAGAAAAACCACTCTTCACCCGGGATGCATCCCAGTTG AAGGGAACATTCCTCAGCACCACCCTAAAAAAGAGCAACATGGGCTTTGGATTTACCATCATTGGTGGAG ACGAGCCTGATGAGTTTCTGCAGGTGAAAAGTGTGATTCCGGATGGGCCTGCAGCACAGGATGGAAAAAT GGAAACAGGTGATGTCATTGTCTATATTAATGAAGTTTGTGTCCTTGGACACACTCATGCAGATGTTGTC AAACTTTTCCAGTCTGTTCCTATTGGTCAGAGTGTCAACCTGGTGTTGTGTCGTGGCTACCCTTTGCCCT TTGATCCTGAAGACCCTGCTAACAGCATGGTGCCACCCCTTGCAATAATGGAGAGGCCACCTCCAGTGAT GGTCAATGGAAGACACAACTATGAAACATATTTGGAGTACATTTCTCGGACCTCACAGTCAGTTCCAGAT ATAACAGATCGGCCGCCTCATTCTCTGCACTCCATGCCAACTGATGGTCAGCTAGACGGCACGTATCCAC CGCCCGTCCATGATGACAATGTGTCTATGGCTTCATCTGGGGCCACCCAAGCTGAACTTATGACCTTAAC CATTGTGAAAGGTGCCCAGGGCTTCGGCTTCACTATTGCCGACAGTCCTACAGGACAGCGGGTGAAACAA ATACTTGACATTCAGGGATGCCCTGGCCTGTGTGAAGGCGACCTCATTGTTGAGATCAACCAGCAGAATG TACAGAACCTGAGCCATACAGAAGTAGTGGATATACTTAAGGACTGTCCCATTGGAAGTGAAACTTCTTT GATTATCCATCGAGGAGGTTTCTTTTCTCCATGGAAAACTCCAAAGCCTATAATGGACCGATGGGAGAAT CAAGGCAGTCCTCAAACGAGTTTATCTGCTCCGGCCATACCGCAGAACCTGCCCTTCCCACCTGCCCTTC ACAGGAGCTCCTTTCCTGACTCAACAGAGGCCTTTGACCCACGGAAGCCTGATCCATATGAGCTCTACGA GAAATCTAGGGCCATTTATGAAAGTAGGCGTCCAGATTATAAGGAATTGGATGTTCATCTTCGGAGGATG GAGTCTGGATTTGGCTTCAGAATCCTCGGGGGAGATGAGCCTGGACAGCCTATTTTGATTGGAGCTGTCA TTGCCATGGGCTCAGCCGACAGAGATGGCCGCCTTCACCCAGGAGATGAGCTTGTGTATGTTGATGGGAT TCCAGTAGCCGGCAAAACCCACCGCTATGTCATCGACCTCATGCACCACGCAGCCCGCAATGGGCAGGTC AACCTCACTGTGAGAAGAAAGGTGCTATGTGGAGGGGAGCCCTGCCCAGAGAACGGGAGAAGTCCAGGCT CTGTATCCACCCACCACAGCTCTCCACGCAGTGACTACGCAACCTACACCAACAGCAACCACGCTGCCCC CAGTAGCAATGCCTCTCCCCCTGAAGGCTTCGCCTCCCACAGCCTGCAGACCAGTGATGTGGTCATTCAC CGCAAAGAGAATGAGGGCTTCGGCTTTGTCATCATCAGCTCCCTGAACAGGCCTGAGTCTGGATCCACTA TAACTGTGCCCCATAAAATCGGACGCATCATTGATGGGAGTCCTGCAGATCGCTGTGCAAAACTAAAAGT GGGAGACCGGATCCTAGCAGTGAATGGCCAGTCTATCATCAACATGCCTCACGCTGACATCGTGAAGCTC ATCAAGGATGCAGGTCTTAGTGTCACCCTTCGCATCATTCCTCAGGAGGAGCTCAACAGCCCCACCTCGG CACCCAGCTCAGAGAAGCAGAGTCCCATGGCGCAGCAGAGTCCCCTGGCACAGCAGAGTCCCCTGGCCCA GCCAAGCCCAGCCACCCCCAACAGCCCCATCGCCCAGCCAGCACCACCTCAACCACTTCAGCTGCAAGGA CACGAAAATAGTTACAGGTCGGAAGTGAAAGCAAGGCAAGATGTGAAACCAGACATCCGACAGCCTCCAT TCACAGACTACAGGCAGCCCCCGCTGGATTACAGGCAACCCCCAGGAGGGGACTACCAGCAGCCCCCACC CTTGGACTACAGGCAGCCTCCCCTACTGGACTACAGGCAGCACTCCCCCGACACCAGGCAGTACCCTCTG TCGGACTACAGACAACCCCAGGATTTTGATTATTTCACTGTGGACATGGAGAAAGGAGCCAAAGGATTTG GATTCAGCATTCGTGGAGGAAGGGAATACAAAATGGATTTGTATGTGTTGAGACTGGCAGAAGATGGACC AGCAATAAGGAATGGGAGGATGAGGGTAGGAGATCAAATCATTGAAATCAATGGGGAAAGCACAAGGGAC ATGACACATGCCAGAGCAATAGAACTCATCAAATCTGGAGGAAGACGAGTGAGGCTGCTGCTCAAGAGAG GCACGGGACAGGTCCCAGAATATGACGAACCCGCCCCCTGGAGTTCTCCCGCTGCCGCCGCCCCAGGTCT GCCGGAAGTAGGCGTCTCCCTGGACGACGGCCTCGCTCCATTCTCTCCATCACATCCAGCCCCACCCTCC GACCCTTCCCACCAGATAAGCCCAGGCCCAACTTGGGATATCAAACGGGAACACGACGTTAGGAAACCAA AGGAGCTTTCAGCCTGCGGCCAGAAGAAGCAGCGCCTCGGGGAGCAGAGGGAGCGCTCGGCGAGTCCGCA GAGGGCCGCGCGGCCGAGGCTCGAGGAGGCGCCCGGCGGCCAGGGGCGGCCCGAGGCCGGCAGGCCCGCC TCGGAGGCCAGGGCGCCCGGGCTCGCGGCGGCAGACGCGGCGGACGCGGCGCGGGCGGGCGGGAAGGAGG CGCCCCGTGCGGCGGCGGGCTCCGAGCTCTGCCGGCGCGAAGGCCCGGGGGCTGCGCCGGCGTTTGCCGG CCCGGGCGGCGGCGGCAGCGGCGCGCTGGAGGCCGAGGGCAGGGCGGGTGCGCGCGCGGGTCCCCGGCCG GGCCCGCGACCCCCGGGGGGCGCCCCGGCGCGCAAGGCCGCCGTCGCGCCGGGGCCCTGGAAGGTGCCGG GTTCTGACAAGCTGCCCAGCGTCCTCAAACCCGGCGCCTCGGCCGCCAGCAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240148 representing NM_001301128 Red=Cloning site Green=Tags(s) MSKSLKKKSHWTSKVHESVIGRNPEGQLGFELKGGAENGQFPYLGEVKPGKVAYESGSKLVSEELLLEVN ETPVAGLTIRDVLAVIKHCKDPLRLKCVKQGGIVDKDLRHYLNLRFQKGSVDHELQQIIRDNLYLRTVPC TTRPHKEGEVPGVDYIFITVEDFMELEKSGALLESGTYEDNYYGTPKPPAEPAPLLLNVTDQILPGATPS AEGKRKRNKSVSNMEKASIEPPEEEEEERPVVNGNGVVVTPESSEHEDKSAGASGEMPSQPYPAPVYSQP EELKEQMDDTKPTKPEDNEEPDPLPDNWEMAYTEKGEVYFIDHNTKTTSWLDPRLAKKAKPPEECKENEL PYGWEKIDDPIYGTYYVDHINRRTQFENPVLEAKRKLQQHNMPHTELGTKPLQAPGFREKPLFTRDASQL KGTFLSTTLKKSNMGFGFTIIGGDEPDEFLQVKSVIPDGPAAQDGKMETGDVIVYINEVCVLGHTHADVV KLFQSVPIGQSVNLVLCRGYPLPFDPEDPANSMVPPLAIMERPPPVMVNGRHNYETYLEYISRTSQSVPD ITDRPPHSLHSMPTDGQLDGTYPPPVHDDNVSMASSGATQAELMTLTIVKGAQGFGFTIADSPTGQRVKQ ILDIQGCPGLCEGDLIVEINQQNVQNLSHTEVVDILKDCPIGSETSLIIHRGGFFSPWKTPKPIMDRWEN QGSPQTSLSAPAIPQNLPFPPALHRSSFPDSTEAFDPRKPDPYELYEKSRAIYESRRPDYKELDVHLRRM ESGFGFRILGGDEPGQPILIGAVIAMGSADRDGRLHPGDELVYVDGIPVAGKTHRYVIDLMHHAARNGQV NLTVRRKVLCGGEPCPENGRSPGSVSTHHSSPRSDYATYTNSNHAAPSSNASPPEGFASHSLQTSDVVIH RKENEGFGFVIISSLNRPESGSTITVPHKIGRIIDGSPADRCAKLKVGDRILAVNGQSIINMPHADIVKL IKDAGLSVTLRIIPQEELNSPTSAPSSEKQSPMAQQSPLAQQSPLAQPSPATPNSPIAQPAPPQPLQLQG HENSYRSEVKARQDVKPDIRQPPFTDYRQPPLDYRQPPGGDYQQPPPLDYRQPPLLDYRQHSPDTRQYPL SDYRQPQDFDYFTVDMEKGAKGFGFSIRGGREYKMDLYVLRLAEDGPAIRNGRMRVGDQIIEINGESTRD MTHARAIELIKSGGRRVRLLLKRGTGQVPEYDEPAPWSSPAAAAPGLPEVGVSLDDGLAPFSPSHPAPPS DPSHQISPGPTWDIKREHDVRKPKELSACGQKKQRLGEQRERSASPQRAARPRLEEAPGGQGRPEAGRPA SEARAPGLAAADAADAARAGGKEAPRAAAGSELCRREGPGAAPAFAGPGGGGSGALEAEGRAGARAGPRP GPRPPGGAPARKAAVAPGPWKVPGSDKLPSVLKPGASAASR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001301128
ORF Size	4323 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301128.2
RefSeq Size	6845 bp
RefSeq ORF	4326 bp
Locus ID	9863
UniProt ID	Q86UL8
Protein Families	Druggable Genome
Protein Pathways	Tight junction
MW	157.7 kDa
Gene Summary	The protein encoded by this gene interacts with atrophin-1. Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. This encoded protein is characterized by two WW domains, a guanylate kinase-like domain, and multiple PDZ domains. It has structural similarity to the membrane-associated guanylate kinase homologue (MAGUK) family. [provided by RefSeq, Jul 2008]

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