LARG (ARHGEF12) (NM_001301084) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	LARG; PRO2792
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240147 representing NM_001301084 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGCTGGAGTACAGACAGGTGATCGAATCATCAAGGTGAATGGAACTCTGGTGACTCATTCAAATC ATCTGGAGGTGGTGAAGCTAATCAAATCTGGTTCCTATGTAGCTCTCACTGTTCAGGGACGCCCACCTGG GTCGCCCCAGATTCCACTTGCCGACTCTGAAGTAGAGCCGTCAGTCATTGGACATATGTCTCCCATCATG ACATCTCCTCATTCACCTGGAGCATCTGGGAATATGGAGAGAATCACTAGTCCTGTGCTCATGGGGGAGG AAAACAATGTGGTTCATAACCAGAAAGTAGAAATTCTGAGAAAAATGTTACAGAAAGAACAGGAACGGCT ACAGTTATTGCAGGAAGATTACAACCGAACACCTGCCCAAAGATTGCTAAAAGAGATCCAAGAGGCCAAG AAACACATTCCTCAGCTGCAAGAGCAGTTATCCAAAGCCACAGGCTCTGCTCAGGATGGAGCTGTAGTTA CACCCTCCAGACCTTTAGGGGACACCCTAACAGTCAGTGAGGCAGAAACAGATCCTGGAGATGTACTGGG CAGGACTGACTGTAGCAGTGGAGATGCTTCTCGGCCCAGTAGTGACAATGCAGATAGTCCCAAGAGTGGC CCAAAAGAGAGAATTTATCTAGAGGAAAACCCAGAGAAAAGTGAAACAATTCAGGACACTGACACTCAAT CACTTGTCGGAAGTCCCTCAACCCGTATAGCACCTCATATTATTGGAGCAGAAGATGATGATTTTGGTAC TGAACATGAACAGATCAATGGACAGTGCAGCTGTTTCCAGAGCATTGAATTACTAAAATCTCGCCCGGCT CATTTGGCTGTTTTCTTACACCATGTAGTTTCACAATTTGACCCTGCGACTTTGCTCTGTTATCTCTATT CAGACCTGTATAAACATACCAATTCCAAAGAAACTCGTCGCATCTTCCTTGAGTTTCATCAGTTCTTTCT AGATCGATCAGCACACCTGAAAGTTTCTGTTCCTGATGAAATGTCTGCAGATCTAGAAAAGAGAAGACCT GAGCTCATTCCTGAGGATCTGCATCGCCACTATATCCAAACTATGCAAGAAAGAGTCCATCCAGAAGTTC AAAGGCACTTAGAAGATTTTCGGCAGAAACGTAGTATGGGACTGACCTTGGCTGAAAGCGAGCTGACTAA ACTTGATGCAGAGCGAGACAAGGACCGATTGACTTTGGAGAAGGAGCGGACATGTGCAGAACAGATTGTT GCCAAAATTGAAGAAGTATTGATGACTGCTCAGGCTGTAGAGGAAGATAAGAGCTCCACCATGCAGTATG TTATTCTCATGTATATGAAGCATTTGGGAGTAAAAGTGAAAGAGCCTCGAAATTTGGAGCACAAACGGGG TCGGATTGGATTTCTTCCCAAAATCAAGCAAAGTATGAAGAAAGATAAAGAAGGGGAAGAAAAAGGGAAG CGAAGAGGATTCCCCAGCATCCTGGGACCCCCACGGAGACCAAGCCGTCATGACAACAGTGCAATTGGCA GAGCCATGGAACTACAGAAGGCGCGCCACCCTAAGCACTTATCCACACCCTCATCTGTGAGTCCTGAACC TCAGGACTCTGCCAAGTTGCGCCAGAGTGGGTTAGCAAATGAAGGAACAGACGCTGGATACCTGCCTGCC AATTCCATGTCTTCTGTAGCTTCAGGGGCCTCTTTTTCCCAGGAAGGAGGGAAAGAGAATGATACAGGAT CAAAGCAAGTTGGAGAAACATCAGCACCTGGAGACACCTTAGATGGCACACCTCGTACTCTCAATACTGT CTTTGATTTCCCACCACCTCCATTAGACCAAGTGCAGGAGGAGGAATGTGAAGTAGAAAGGGTGACTGAA CATGGGACACCAAAGCCCTTTCGAAAGTTTGACAGTGTAGCTTTTGGAGAAAGTCAAAGTGAGGATGAAC AATTTGAAAATGACTTAGAGACAGATCCACCCAACTGGCAGCAGCTTGTTAGTCGAGAAGTGTTACTGGG ACTAAAACCTTGTGAAATCAAAAGACAGGAAGTGATTAATGAATTGTTCTACACTGAAAGAGCTCATGTT CGAACACTGAAGGTTCTTGATCAAGTGTTCTATCAGCGAGTATCCAGAGAAGGAATTCTGTCACCCTCAG AGCTACGGAAAATTTTTTCAAACTTGGAAGATATTCTTCAACTTCATATTGGATTGAATGAACAAATGAA GGCTGTTCGAAAGAGAAATGAGACCTCTGTTATCGATCAGATTGGGGAAGATTTGCTGACATGGTTCAGC GGACCAGGAGAGGAGAAATTGAAACATGCTGCTGCTACCTTTTGCAGTAACCAACCTTTCGCCCTGGAAA TGATCAAATCTCGTCAGAAAAAGGATTCTCGATTTCAGACTTTTGTGCAAGATGCTGAAAGTAATCCACT GTGTCGTCGTCTTCAACTGAAGGATATTATTCCCACTCAAATGCAAAGGCTTACTAAGTACCCACTTCTG TTGGATAATATTGCCAAATACACAGAATGGCCAACAGAAAGGGAGAAGGTGAAGAAAGCTGCAGATCACT GTCGTCAGATCTTAAATTATGTAAATCAGGCTGTCAAGGAGGCAGAAAACAAGCAGCGCCTAGAAGATTA TCAGCGTCGCCTTGATACCTCCAGCCTGAAGTTGTCAGAGTACCCAAATGTTGAAGAGCTCAGGAATTTG GATTTAACAAAAAGGAAGATGATTCATGAAGGGCCATTGGTTTGGAAGGTGAATAGAGATAAAACTATTG ATTTATACACGTTGCTGCTGGAAGACATTCTTGTATTGTTACAAAAGCAGGATGATAGACTGGTTTTAAG GTGTCATAGTAAGATTCTGGCATCTACAGCTGATAGCAAACACACGTTTAGCCCTGTCATTAAGTTGAGT ACAGTGTTGGTTCGACAAGTGGCAACAGATAACAAAGCTTTATTCGTCATTTCCATGTCAGACAATGGCG CTCAGATTTATGAACTGGTGGCACAGACAGTTTCTGAAAAGACTGTCTGGCAGGACCTAATCTGTCGGAT GGCTGCATCAGTGAAGGAGCAATCCACAAAGCCAATTCCATTACCACAGTCAACACCTGGCGAAGGAGAT AATGATGAAGAAGATCCTTCAAAATTAAAAGAGGAGCAGCATGGCATTTCAGTCACTGGTTTGCAGAGTC CAGACAGAGATTTGGGATTAGAATCTACCTTAATATCGTCAAAACCTCAGTCTCATTCACTGAGTACCTC TGGGAAATCAGAGGTACGTGATCTGTTTGTGGCTGAGAGACAGTTTGCAAAGGAACAACATACAGATGGG ACACTAAAGGAAGTTGGAGAAGATTATCAAATCGCAATCCCAGATTCACACCTGCCTGTCTCAGAAGAAC GGTGGGCATTGGATGCACTAAGAAATTTGGGTTTGTTGAAGCAGTTGCTGGTGCAACAGCTAGGTTTGAC TGAGAAGAGCGTTCAGGAAGACTGGCAACATTTCCCAAGATACAGAACAGCCTCTCAGGGGCCGCAGACA GACAGTGTCATCCAGAACTCTGAAAATATTAAGGCCTATCATTCTGGTGAAGGACATATGCCCTTTAGAA CTGGAACTGGTGACATTGCAACTTGTTACAGTCCACGGACTTCAACTGAATCTTTTGCTCCACGGGATTC AGTGGGACTGGCACCCCAGGATAGCCAGGCAAGTAACATTTTAGTAATGGACCACATGATTATGACCCCA GAGATGCCTACCATGGAGCCAGAAGGGGGTCTTGATGACAGTGGAGAGCACTTTTTTGATGCCCGTGAAG CACATAGTGATGAGAATCCATCAGAAGGTGATGGAGCAGTTAACAAGGAAGAGAAGGATGTTAATTTACG CATCTCAGGAAACTATTTGATCCTTGATGGCTATGACCCAGTGCAGGAGAGTTCCACAGATGAGGAGGTT GCTTCCTCACTTACCCTGCAGCCCATGACAGGCATCCCTGCTGTGGAATCCACCCACCAGCAGCAACATT CTCCTCAGAATACTCACTCCGATGGGGCAATTTCACCATTCACCCCCGAATTTCTGGTCCAGCAGCGCTG GGGAGCTATGGAGTATTCCTGTTTTGAGATCCAGAGTCCCTCCTCTTGTGCAGATTCACAGAGCCAGATC ATGGAGTACATTCATAAGATAGAGGCTGACCTTGAACACTTAAAGAAGGTGGAGGAAAGTTACACCATTC TTTGCCAAAGGCTGGCTGGATCAGCCCTCACAGACAAGCACTCAGATAAAAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240147 representing NM_001301084 Red=Cloning site Green=Tags(s) MRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIM TSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAK KHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDASRPSSDNADSPKSG PKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPA HLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRP ELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIV AKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGK RRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPA NSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTE HGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHV RTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFS GPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLL LDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNL DLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLS TVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGD NDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDG TLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQT DSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTP EMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEV ASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQI MEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001301084
ORF Size	4323 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301084.2
RefSeq Size	9655 bp
RefSeq ORF	4326 bp
Locus ID	23365
UniProt ID	Q9NZN5
Protein Pathways	Axon guidance, Regulation of actin cytoskeleton, Vascular smooth muscle contraction
MW	162.4 kDa
Gene Summary	Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

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