RPB2 (POLR2B) (NM_001303268) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	hRPB140; POL2RB; RPB2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239962 representing NM_001303268 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGATTGTAATCAGCCACGATATTTGCTGAAGTTTGAACAAATTTATCTTTCCAAGCCTACCCATT GGGAAAGAGATGGTGCTCCTTCACCAATGATGCCCAATGAAGCTAGATTAAGGAATCTCACGTATTCTGC TCCGCTTTATGTTGATATAACAAAAACAGTCATTAAAGAAGGTGAAGAACAACTTCAGACTCAGCATCAG AAAACTTTTATAGGAAAAATTCCAATTATGTTGCGGTCAACTTACTGCCTTTTGAATGGCTTGACAGATC GTGATCTTTGTGAGTTAAATGAATGCCCTTTGGATCCTGGTGGCTATTTCATTATTAATGGATCAGAAAA GGTTCTGATTGCCCAAGAGAAAATGGCAACAAACACAGTTTATGTGTTTGCCAAAAAGGATTCTAAATAT GCCTACACAGGAGAGTGTAGATCATGTCTTGAGAATTCTTCCCGACCCACCAGTACTATATGGGTTAGCA TGCTGGCAAGAGGAGGACAGGGTGCCAAGAAGAGTGCTATTGGTCAGCGCATTGTGGCAACTCTACCATA TATCAAGCAAGAAGTTCCCATCATTATTGTGTTCAGAGCATTAGGTTTTGTGTCCGACAGAGATATTTTA GAACATATTATTTATGATTTTGAAGATCCAGAGATGATGGAAATGGTTAAACCTTCTCTCGATGAAGCTT TTGTCATCCAAGAACAGAATGTTGCACTAAATTTCATTGGTTCAAGAGGAGCAAAGCCTGGTGTTACTAA AGAGAAAAGAATTAAATATGCAAAGGAAGTTTTACAAAAAGAAATGCTCCCTCATGTTGGTGTCAGTGAT TTTTGTGAGACCAAAAAAGCCTATTTCTTGGGATACATGGTTCATAGGTTACTTCTGGCAGCTTTGGGTA GAAGAGAACTAGATGACAGAGATCACTATGGAAACAAGAGATTGGATCTTGCTGGGCCGCTGCTTGCATT CTTATTTAGAGGTATGTTTAAGAATTTGCTTAAAGAAGTGCGGATCTATGCACAGAAATTTATTGATCGA GGAAAGGATTTTAACTTGGAGTTGGCAATTAAAACACGGATCATATCTGATGGCCTAAAATACTCTTTAG CTACTGGAAACTGGGGTGATCAAAAGAAAGCTCATCAAGCCAGAGCTGGAGTATCTCAGGTGTTAAACCG CCTGACTTTTGCGTCTACTCTTTCTCACCTGCGTCGTTTAAATTCTCCTATTGGTAGAGACGGCAAGCTA GCAAAACCAAGACAGTTGCATAATACGTTGTGGGGAATGGTGTGTCCTGCCGAGACCCCAGAGGGCCATG CTGTAGGACTTGTGAAGAATTTAGCCTTGATGGCGTATATTTCAGTTGGATCTCAACCATCTCCAATTCT GGAATTTTTAGAAGAATGGAGTATGGAAAATTTAGAAGAAATTTCTCCTGCAGCTATTGCTGATGCAACC AAGATTTTTGTTAATGGCTGCTGGGTTGGAATACATAAAGATCCCGAACAACTTATGAACACCCTAAGGA AATTGAGACGTCAGATGGACATCATTGTGTCTGAAGTTTCTATGATCAGAGATATTCGAGAGAGGGAGAT TCGGATCTATACGGATGCAGGCCGTATTTGTAGACCACTTCTGATTGTGGAAAAACAAAAGCTACTTTTG AAGAAGAGGCATATTGACCAATTGAAAGAGAGAGAATATAACAACTATAGTTGGCAGGATCTTGTGGCCA GTGGGGTAGTGGAGTATATTGATACCCTGGAAGAAGAAACAGTGATGCTTGCAATGACTCCAGATGATTT ACAGGAGAAAGAAGTAGCTTATTGTTCCACATATACACACTGTGAGATTCATCCCTCAATGATCCTTGGT GTCTGTGCATCTATTATTCCCTTTCCTGATCATAACCAGTCCCCTAGAAACACATACCAGTCTGCTATGG GTAAGCAGGCTATGGGAGTTTACATCACCAACTTCCATGTTCGCATGGACACATTGGCCCATGTTCTCTA TTATCCTCAAAAGCCACTTGTGACTACACGGTCTATGGAATATCTACGATTTAGAGAGCTGCCAGCAGGC ATCAACTCAATTGTGGCCATTGCATCATACACTGGATATAATCAGGAAGACTCTGTTATCATGAATCGTT CAGCTGTAGACCGCGGCTTCTTCAGGTCTGTTTTCTATCGCTCATACAAAGAACAGGAGTCTAAAAAAGG ATTTGATCAAGAAGAAGTTTTTGAGAAGCCTACACGTGAAACATGCCAGGGCATGAGGCATGCCATTTAC GACAAGCTGGATGATGATGGTTTGATAGCTCCAGGGGTTCGTGTATCAGGAGATGATGTTATTATAGGCA AAACAGTCACCTTGCCTGAAAATGAAGATGAATTGGAGAGCACCAATAGACGCTATACCAAGAGAGACTG TAGCACTTTTCTCAGAACTAGCGAGACGGGCATTGTGGATCAGGTTATGGTAACTCTCAATCAGGAAGGA TATAAATTTTGTAAAATAAGGGTACGCTCTGTTAGGATTCCACAGATTGGAGACAAATTTGCTAGTCGAC ATGGTCAAAAGGGTACTTGTGGTATTCAGTATAGACAAGAGGATATGCCTTTCACCTGTGAAGGTATCAC CCCTGATATCATCATCAATCCCCATGCCATCCCCTCTCGTATGACTATTGGTCACTTAATTGAATGCCTT CAAGGGAAGGTATCGGCTAACAAGGGTGAAATTGGTGATGCCACTCCATTTAATGATGCTGTTAACGTGC AGAAGATTTCTAATCTTTTATCTGATTATGGCTATCATCTCAGAGGAAATGAGGTCCTGTACAATGGGTT CACTGGTCGAAAAATCACATCACAAATATTTATTGGCCCCACTTATTACCAGCGTTTGAAGCATATGGTG GATGATAAGATTCACTCTCGTGCTAGGGGACCTATTCAGATCCTCAATAGACAGCCCATGGAGGGTAGAT CTCGTGATGGTGGCCTGCGTTTTGGAGAAATGGAACGAGATTGTCAGATTGCCCATGGAGCAGCCCAGTT TTTAAGGGAAAGATTGTTTGAGGCATCAGATCCATATCAGGTTCATGTTTGCAATCTTTGTGGAATAATG GCGATTGCCAACACCAGGACCCATACATATGAATGCAGGGGCTGCCGCAATAAAACCCAGATTTCTTTGG TGCGAATGCCTTACGCATGCAAACTATTGTTTCAGGAACTTATGTCTATGAGTATTGCACCGCGAATGAT GAGTGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239962 representing NM_001303268 Red=Cloning site Green=Tags(s) MLDCNQPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQ KTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKY AYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDRDIL EHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSD FCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDR GKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIGRDGKL AKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAAIADAT KIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLL KKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILG VCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAG INSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGMRHAIY DKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVTLNQEG YKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECL QGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQRLKHMV DDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIM AIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001303268
ORF Size	3297 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001303268.1, NP_001290197.1
RefSeq Size	3911 bp
RefSeq ORF	3300 bp
Locus ID	5431
UniProt ID	P30876
Protein Families	Transcription Factors
Protein Pathways	Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
MW	125.6 kDa
Gene Summary	This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

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