Eph receptor A7 (EPHA7) (NM_001288629) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	EHK-3; EHK3; EK11; HEK11
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239862 representing NM_001288629 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTTTTCAAACTCGGTACCCTTCATGGATTATTTTATGCTACATCTGGCTGCTCCGCTTTGCACACA CAGGGGAGGCGCAGGCTGCGAAGGAAGTACTACTGCTGGATTCTAAAGCACAACAAACAGAGTTGGAGTG GATTTCCTCTCCACCCAATGGGTGGGAAGAAATTAGTGGTTTGGATGAGAACTATACCCCGATACGAACA TACCAGGTGTGCCAAGTCATGGAGCCCAACCAAAACAACTGGCTGCGGACTAACTGGATTTCCAAAGGCA ATGCACAAAGGATTTTTGTAGAATTGAAATTCACCCTGAGGGATTGTAACAGTCTTCCTGGAGTACTGGG AACTTGCAAGGAAACATTTAATTTGTACTATTATGAAACAGACTATGACACTGGCAGGAATATAAGAGAA AACCTCTATGTAAAAATAGACACCATTGCTGCAGATGAAAGTTTTACCCAAGGTGACCTTGGTGAAAGAA AGATGAAGCTTAACACTGAGGTGAGAGAGATTGGACCTTTGTCCAAAAAGGGATTCTATCTTGCCTTTCA GGATGTAGGGGCTTGCATAGCTTTGGTTTCTGTCAAAGTGTACTACAAGAAGTGCTGGTCCATTATTGAG AACTTAGCTATCTTTCCAGATACAGTGACTGGTTCAGAATTTTCCTCTTTAGTCGAGGTTCGAGGGACAT GTGTCAGCAGTGCAGAGGAAGAAGCGGAAAACGCCCCCAGGATGCACTGCAGTGCAGAAGGAGAATGGTT AGTGCCCATTGGAAAATGTATCTGCAAAGCAGGCTACCAGCAAAAAGGAGACACTTGTGAACCCTGTGGC CGTGGGTTCTACAAGTCTTCCTCTCAAGATCTTCAGTGCTCTCGTTGTCCAACTCACAGTTTTTCTGATA AAGAAGGCTCCTCCAGATGTGAATGTGAAGATGGGTATTACAGGGCTCCATCTGACCCACCATACGTTGC GTGCACAAGGCCTCCATCTGCACCACAGAACCTCATTTTCAACATCAACCAAACCACAGTAAGTTTGGAA TGGAGTCCTCCTGCAGACAATGGGGGAAGAAACGATGTGACCTACAGAATATTGTGTAAGCGGTGCAGTT GGGAGCAGGGCGAATGTGTTCCCTGTGGGAGTAACATTGGATACATGCCCCAGCAGACTGGATTAGAGGA TAACTATGTCACTGTCATGGACCTGCTAGCCCACGCTAATTATACTTTTGAAGTTGAAGCTGTAAATGGA GTTTCTGACTTAAGCCGATCCCAGAGGCTCTTTGCTGCTGTCAGTATCACCACTGGTCAAGCAGCTCCCT CGCAAGTGAGTGGAGTAATGAAGGAGAGAGTACTGCAGCGGAGTGTCGAGCTTTCCTGGCAGGAACCAGA GCATCCCAATGGAGTCATCACAGAATATGAAATCAAGTATTACGAGAAAGATCAAAGGGAACGGACCTAC TCAACAGTAAAAACCAAGTCTACTTCAGCCTCCATTAATAATCTGAAACCAGGAACAGTGTATGTTTTCC AGATTCGGGCTTTTACTGCTGCTGGTTATGGAAATTACAGTCCCAGACTTGATGTTGCTACACTAGAGGA AGCTACAGCTACAGCTGTCTCCAGTGAACAGAATCCTGTTATTATCATTGCTGTGGTTGCTGTAGCTGGG ACCATCATTTTGGTGTTCATGGTCTTTGGCTTCATCATTGGGAGAAGGCACTGTGGTTATAGCAAAGCTG ACCAAGAAGGCGATGAAGAGCTTTACTTTCATTTTAAATTTCCAGGCACCAAAACCTACATTGACCCTGA AACCTATGAGGACCCAAATAGAGCTGTCCATCAATTCGCCAAGGAGCTAGATGCCTCCTGTATTAAAATT GAGCGTGTGATTGGTGCAGGAGAATTCGGTGAAGTCTGCAGTGGCCGTTTGAAACTTCCAGGGAAAAGAG ATGTTGCAGTAGCCATAAAAACCCTGAAAGTTGGTTACACAGAAAAACAAAGGAGAGACTTTTTGTGTGA AGCAAGCATCATGGGGCAGTTTGACCACCCGAATGTTGTCCATTTGGAAGGGGTTGTTACAAGAGGGAAA CCAGTCATGATAGTAATAGAGTTCATGGAAAATGGAGCCCTAGATGCATTTCTCAGGAAACATGATGGGC AATTTACAGTCATTCAGTTAGTAGGAATGCTGAGAGGAATTGCTGCTGGAATGAGATATTTGGCTGATAT GGGATATGTTCACAGGGACCTTGCAGCTCGCAATATTCTTGTCAACAGCAATCTCGTTTGTAAAGTGTCA GATTTTGGCCTGTCCCGAGTTATAGAGGATGATCCAGAAGCTGTCTATACAACTACTGGTGGAAAAATTC CAGTAAGGTGGACAGCACCCGAAGCCATCCAGTACCGGAAATTCACATCAGCCAGTGATGTATGGAGCTA TGGAATAGTCATGTGGGAAGTTATGTCTTATGGAGAAAGACCTTATTGGGACATGTCAAATCAAGATGTT ATAAAAGCAATAGAAGAAGGTTATCGTTTACCAGCACCCATGGACTGCCCAGCTGGCCTTCACCAGCTAA TGTTGGATTGTTGGCAAAAGGAGCGTGCTGAAAGGCCAAAATTTGAACAGATAGTTGGAATTCTAGACAA AATGATTCGAAACCCAAATAGTCTGAAAACTCCCCTGGGAACTTGTAGTAGGCCAATAAGCCCTCTTCTG GATCAAAACACTCCTGATTTCACTACCTTTTGTTCAGTTGGAGAATGGCTACAAGCTATTAAGATGGAAA GATATAAAGATAATTTCACGGCAGCTGGCTACAATTCCCTTGAATCAGTAGCCAGGATGACTATTGAGGA TGTGATGAGTTTAGGGATCACACTGGTTGGTCATCAAAAGAAAATCATGAGCAGCATTCAGACTATGAGA GCACAAATGCTACATTTACATGGAACTGGCATTCAAGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239862 representing NM_001288629 Red=Cloning site Green=Tags(s) MVFQTRYPSWIILCYIWLLRFAHTGEAQAAKEVLLLDSKAQQTELEWISSPPNGWEEISGLDENYTPIRT YQVCQVMEPNQNNWLRTNWISKGNAQRIFVELKFTLRDCNSLPGVLGTCKETFNLYYYETDYDTGRNIRE NLYVKIDTIAADESFTQGDLGERKMKLNTEVREIGPLSKKGFYLAFQDVGACIALVSVKVYYKKCWSIIE NLAIFPDTVTGSEFSSLVEVRGTCVSSAEEEAENAPRMHCSAEGEWLVPIGKCICKAGYQQKGDTCEPCG RGFYKSSSQDLQCSRCPTHSFSDKEGSSRCECEDGYYRAPSDPPYVACTRPPSAPQNLIFNINQTTVSLE WSPPADNGGRNDVTYRILCKRCSWEQGECVPCGSNIGYMPQQTGLEDNYVTVMDLLAHANYTFEVEAVNG VSDLSRSQRLFAAVSITTGQAAPSQVSGVMKERVLQRSVELSWQEPEHPNGVITEYEIKYYEKDQRERTY STVKTKSTSASINNLKPGTVYVFQIRAFTAAGYGNYSPRLDVATLEEATATAVSSEQNPVIIIAVVAVAG TIILVFMVFGFIIGRRHCGYSKADQEGDEELYFHFKFPGTKTYIDPETYEDPNRAVHQFAKELDASCIKI ERVIGAGEFGEVCSGRLKLPGKRDVAVAIKTLKVGYTEKQRRDFLCEASIMGQFDHPNVVHLEGVVTRGK PVMIVIEFMENGALDAFLRKHDGQFTVIQLVGMLRGIAAGMRYLADMGYVHRDLAARNILVNSNLVCKVS DFGLSRVIEDDPEAVYTTTGGKIPVRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDV IKAIEEGYRLPAPMDCPAGLHQLMLDCWQKERAERPKFEQIVGILDKMIRNPNSLKTPLGTCSRPISPLL DQNTPDFTTFCSVGEWLQAIKMERYKDNFTAAGYNSLESVARMTIEDVMSLGITLVGHQKKIMSSIQTMR AQMLHLHGTGIQV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001288629
ORF Size	2979 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001288629.2
RefSeq Size	6629 bp
RefSeq ORF	2982 bp
Locus ID	2045
UniProt ID	Q15375
Protein Families	Druggable Genome, Protein Kinase, Transmembrane
Protein Pathways	Axon guidance
MW	112 kDa
Gene Summary	This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Increased expression of this gene is associated with multiple forms of carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.