NMDAR2C (GRIN2C) (NM_001278553) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GluN2C; NMDAR2C; NR2C
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239716 representing NM_001278553 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGGGGCCCTGGGGCCGGCCCTGTTGCTCACCTCGCTCTTCGGTGCCTGGGCAGGGCTGGGTCCGG GGCAGGGCGAGCAGGGCATGACGGTGGCCGTGGTGTTTAGCAGCTCAGGGCCGCCCCAGGCCCAGTTCCG TGCCCGCCTCACCCCCCAGAGCTTCCTGGACCTACCCCTGGAGATCCAGCCGCTCACAGTTGGGGTCAAC ACCACCAACCCCAGCAGCCTCCTCACCCAGATCTGCGGCCTCCTGGGTGCTGCCCACGTCCACGGCATTG TCTTTGAGGACAACGTGGACACCGAGGCGGTGGCCCAGATCCTTGACTTCATCTCCTCCCAGACCCATGT GCCCATCCTCAGCATCAGCGGAGGCTCTGCTGTGGTCCTCACCCCCAAGGAGCCGGGCTCCGCCTTCCTG CAGCTGGGCGTGTCCCTGGAGCAGCAGCTGCAGGTGCTGTTCAAGGTGCTGGAAGAGTACGACTGGAGCG CCTTCGCCGTCATCACCAGCCTGCACCCGGGCCACGCGCTCTTCCTGGAGGGCGTGCGCGCCGTCGCCGA CGCCAGCCACGTGAGTTGGCGGCTGCTGGACGTGGTCACGCTGGAGCTGGGCCCGGGAGGGCCGCGCGCG CGCACGCAGCGCCTGCTGCGCCAGCTCGACGCGCCCGTGTTTGTGGCCTACTGCTCGCGCGAGGAGGCCG AGGTGCTCTTCGCCGAGGCGGCGCAGGCCGGTCTGGTGGGGCCCGGCCACGTGTGGCTGGTGCCCAACCT GGCGCTGGGCAGCACCGATGCGCCCCCCGCCACCTTCCCCGTGGGCCTCATCAGCGTCGTCACCGAGAGC TGGCGCCTCAGCCTGCGCCAGAAGGTGCGCGACGGCGTGGCCATTCTGGCCCTGGGCGCCCACAGCTACT GGCGCCAGCATGGAACCCTGCCAGCCCCGGCCGGGGACTGCCGTGTTCACCCTGGGCCCGTCAGCCCTGC CCGGGAGGCCTTCTACAGGCACCTACTGAATGTCACCTGGGAGGGCCGAGACTTCTCCTTCAGCCCTGGT GGGTACCTGGTCCAGCCCACCATGGTGGTGATCGCCCTCAACCGGCACCGCCTCTGGGAGATGGTGGGGC GCTGGGAGCATGGCGTCCTATACATGAAGTACCCCGTGTGGCCTCGCTACAGTGCCTCTCTGCAGCCTGT GGTGGACAGTCGGCACCTGACGGTGGCCACGCTGGAAGAGCGGCCCTTTGTCATCGTGGAGAGCCCTGAC CCTGGCACAGGAGGCTGTGTCCCCAACACCGTGCCCTGCCGCAGGCAGAGCAACCACACCTTCAGCAGCG GGGACGTGGCCCCCTACACCAAGCTCTGCTGTAAGGGATTCTGCATCGACATCCTCAAGAAGCTGGCCAG AGTGGTCAAATTCTCCTACGACCTGTACCTGGTGACCAACGGCAAGCATGGCAAGCGGGTGCGCGGCGTA TGGAACGGCATGATTGGGGAGGTGTACTACAAGCGGGCAGACATGGCCATCGGCTCCCTCACCATCAATG AGGAACGCTCCGAGATCGTAGACTTCTCTGTACCCTTTGTGGAGACGGGCATCAGTGTGATGGTGGCTCG CAGCAATGGCACCGTCTCCCCCTCGGCCTTCTTGGAGCCATATAGCCCTGCAGTGTGGGTGATGATGTTT GTCATGTGCCTCACTGTGGTGGCCATCACCGTCTTCATGTTCGAGTACTTCAGCCCTGTCAGCTACAACC AGAACCTCACCAGAGGCAAGAAGTCCGGGGGCCCAGCTTTCACTATCGGCAAGTCCGTGTGGCTGCTGTG GGCGCTGGTCTTCAACAACTCAGTGCCCATCGAGAACCCGCGGGGCACCACCAGCAAGATCATGGTTCTG GTCTGGGCCTTCTTTGCTGTCATCTTCCTCGCCAGCTACACGGCCAACCTGGCCGCCTTCATGATCCAAG AGCAATACATCGACACTGTGTCGGGCCTCAGTGACAAGAAGTTTCAGCGGCCTCAAGATCAGTACCCACC TTTCCGCTTCGGCACGGTGCCCAACGGCAGCACGGAGCGGAACATCCGCAGTAACTACCGTGACATGCAC ACCCACATGGTCAAGTTCAACCAGCGCTCGGTGGAGGACGCGCTCACCAGCCTCAAGATGGGGAAGCTGG ATGCCTTCATCTATGATGCTGCTGTCCTCAACTACATGGCAGGCAAGGACGAGGGCTGCAAGCTGGTCAC CATTGGGTCTGGCAAGGTCTTTGCTACCACTGGCTACGGCATCGCCATGCAGAAGGACTCCCACTGGAAG CGGGCCATAGACCTGGCGCTCTTGCAGTTCCTGGGGGACGGAGAGACACAGAAACTGGAGACAGTGTGGC TCTCAGGGATCTGCCAGAATGAGAAGAACGAGGTGATGAGCAGCAAGCTGGACATCGACAACATGGCAGG CGTCTTCTACATGCTGCTGGTGGCCATGGGGCTGGCCCTGCTGGTCTTCGCCTGGGAGCACCTGGTCTAC TGGAAGCTGCGCCACTCGGTGCCCAACTCATCCCAGCTGGACTTCCTGCTGGCTTTCAGCAGGGTGGGTG CCCACCCCTCCCCACACAGGCCAAAGTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239716 representing NM_001278553 Red=Cloning site Green=Tags(s) MGGALGPALLLTSLFGAWAGLGPGQGEQGMTVAVVFSSSGPPQAQFRARLTPQSFLDLPLEIQPLTVGVN TTNPSSLLTQICGLLGAAHVHGIVFEDNVDTEAVAQILDFISSQTHVPILSISGGSAVVLTPKEPGSAFL QLGVSLEQQLQVLFKVLEEYDWSAFAVITSLHPGHALFLEGVRAVADASHVSWRLLDVVTLELGPGGPRA RTQRLLRQLDAPVFVAYCSREEAEVLFAEAAQAGLVGPGHVWLVPNLALGSTDAPPATFPVGLISVVTES WRLSLRQKVRDGVAILALGAHSYWRQHGTLPAPAGDCRVHPGPVSPAREAFYRHLLNVTWEGRDFSFSPG GYLVQPTMVVIALNRHRLWEMVGRWEHGVLYMKYPVWPRYSASLQPVVDSRHLTVATLEERPFVIVESPD PGTGGCVPNTVPCRRQSNHTFSSGDVAPYTKLCCKGFCIDILKKLARVVKFSYDLYLVTNGKHGKRVRGV WNGMIGEVYYKRADMAIGSLTINEERSEIVDFSVPFVETGISVMVARSNGTVSPSAFLEPYSPAVWVMMF VMCLTVVAITVFMFEYFSPVSYNQNLTRGKKSGGPAFTIGKSVWLLWALVFNNSVPIENPRGTTSKIMVL VWAFFAVIFLASYTANLAAFMIQEQYIDTVSGLSDKKFQRPQDQYPPFRFGTVPNGSTERNIRSNYRDMH THMVKFNQRSVEDALTSLKMGKLDAFIYDAAVLNYMAGKDEGCKLVTIGSGKVFATTGYGIAMQKDSHWK RAIDLALLQFLGDGETQKLETVWLSGICQNEKNEVMSSKLDIDNMAGVFYMLLVAMGLALLVFAWEHLVY WKLRHSVPNSSQLDFLLAFSRVGAHPSPHRPKF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278553
ORF Size	2619 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278553.1, NP_001265482.1
RefSeq Size	3269 bp
RefSeq ORF	2622 bp
Locus ID	2905
Protein Families	Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
Protein Pathways	Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Calcium signaling pathway, Long-term potentiation, Neuroactive ligand-receptor interaction
MW	96.4 kDa
Gene Summary	This gene encodes a subunit of the N-methyl-D-aspartate (NMDA) receptor, which is a subtype of ionotropic glutamate receptor. NMDA receptors are found in the central nervous system, are permeable to cations and have an important role in physiological processes such as learning, memory, and synaptic development. The receptor is a tetramer of different subunits (typically heterodimer of subunit 1 with one or more of subunits 2A-D), forming a channel that is permeable to calcium, potassium, and sodium, and whose properties are determined by subunit composition. Alterations in the subunit composition of the receptor are associated with pathophysiological conditions such as Parkinson's disease, Alzheimer's disease, depression, and schizophrenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

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