HSPC302 (TBCK) (NM_001290768) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HSPC302; IHPRF3; TBCKL
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239411 representing NM_001290768 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAAGTAAAAAACCATTGCCTTCTGGCCCCAAATCAGATGTATGGTCTCTTGGAATCATTTTATTTG AGCTTTGTGTGGGAAGAAAATTATTTCAGAGCTTGGATATTTCTGAAAGACTAAAATTTTTGCTTACTTT GGATTGTGTAGATGACACTTTAATAGTTCTGGCTGAAGAGCATGGTTGTTTGGACATTATAAAGGAGCTT CCTGAAACTGTGATAGATCTTTTGAATAAGTGCCTTACCTTCCATCCTTCTAAGAGGCCAACCCCAGATG AATTAATGAAGGACAAAGTATTCAGTGAGGTATCACCTTTATATACCCCCTTTACCAAACCTGCCAGTCT GTTTTCATCTTCTCTGAGATGTGCTGATTTAACTCTGCCTGAGGATATCAGTCAGTTGTGTAAAGATATA AATAATGATTACCTGGCAGAAAGATCTATTGAAGAAGTGTATTACCTTTGGTGTTTGGCTGGAGGTGACT TGGAGAAAGAGCTTGTCAACAAGGAAATCATTCGATCCAAACCACCTATCTGCACACTCCCCAATTTTCT CTTTGAGGATGGTGAAAGCTTTGGACAAGGTCGAGATAGAAGCTCGCTTTTAGATGATACCACTGTGACA TTGTCGTTATGCCAGCTAAGAAATAGATTGAAAGATGTTGGTGGAGAAGCATTTTACCCATTACTTGAAG ATGACCAGTCTAATTTACCTCATTCAAACAGCAATAATGAGTTGTCTGCAGCTGCCACGCTCCCTTTAAT CATCAGAGAGAAGGATACAGAGTACCAACTAAATAGAATTATTCTCTTCGACAGGCTGCTAAAGGCTTAT CCATATAAAAAAAACCAAATCTGGAAAGAAGCAAGAGTTGACATTCCTCCTCTTATGAGAGGTTTAACCT GGGCTGCTCTTCTGGGAGTTGAGGGAGCTATTCATGCCAAGTACGATGCAATTGATAAAGACACTCCAAT TCCTACAGATAGACAAATTGAAGTGGATATTCCTCGCTGTCATCAGTACGATGAACTGTTATCATCACCA GAAGGTCATGCAAAATTTAGGCGTGTATTAAAAGCCTGGGTAGTGTCTCATCCTGATCTTGTGTATTGGC AAGGTCTTGACTCACTTTGTGCTCCATTCCTATATCTAAACTTCAATAATGAAGCCTTGGCTTATGCATG TATGTCTGCTTTTATTCCCAAATACCTGTATAACTTCTTCTTAAAAGACAACTCACATGTAATACAAGAG TATCTGACTGTCTTCTCTCAGATGATTGCATTTCATGATCCAGAGCTGAGTAATCATCTCAATGAGATTG GTTTCATTCCAGATCTCTATGCCATCCCTTGGTTTCTTACCATGTTTACTCATGTATTTCCACTACACAA AATTTTCCACCTCTGGGATACCTTACTACTTGGGAATTCCTCTTTCCCATTCTGTATTGGAGTAGCAATT CTTCAGCAGCTGCGGGACCGGCTTTTGGCTAATGGCTTTAATGAGTGTATTCTTCTCTTCTCCGATTTAC CAGAAATTGACATTGAACGCTGTGTGAGAGAATCTATCAACCTGTTTTGTTGGACTCCTAAAAGTGCTAC TTACAGACAGCATGCTCAACCTCCAAAGCCATCTTCTGACAGCAGTGGAGGCAGAAGTTCGGCACCTTAT TTCTCTGCTGAGTGTCCAGATCCTCCAAAGACAGATCTGTCAAGAGAATCCATCCCATTAAATGACCTGA AGTCAGAAGTATCACCACGGATTTCAGCAGAGGACCTGATTGACTTGTGTGAGCTCACAGTGACAGGCCA CTTCAAAACACCCAGCAAGAAAACAAAGTCCAGTAAACCAAAGCTCCTGGTGGTTGACATCCGGAATAGT GAAGACTTTATTCGTGGTCACATTTCAGGAAGCATCAACATTCCATTCAGTGCTGCCTTCACTGCAGAAG GGGAGCTTACCCAGGGCCCTTACACTGCTATGCTCCAGAACTTCAAAGGGAAGGTCATTGTCATCGTGGG GCATGTGGCAAAACACACAGCTGAGTTTGCAGCTCACCTTGTGAAGATGAAATATCCAAGAATCTGTATT CTAGATGGTGGCATTAATAAAATAAAGCCAACAGGCCTCCTCACCATCCCATCTCCTCAAATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239411 representing NM_001290768 Red=Cloning site Green=Tags(s) MPSKKPLPSGPKSDVWSLGIILFELCVGRKLFQSLDISERLKFLLTLDCVDDTLIVLAEEHGCLDIIKEL PETVIDLLNKCLTFHPSKRPTPDELMKDKVFSEVSPLYTPFTKPASLFSSSLRCADLTLPEDISQLCKDI NNDYLAERSIEEVYYLWCLAGGDLEKELVNKEIIRSKPPICTLPNFLFEDGESFGQGRDRSSLLDDTTVT LSLCQLRNRLKDVGGEAFYPLLEDDQSNLPHSNSNNELSAAATLPLIIREKDTEYQLNRIILFDRLLKAY PYKKNQIWKEARVDIPPLMRGLTWAALLGVEGAIHAKYDAIDKDTPIPTDRQIEVDIPRCHQYDELLSSP EGHAKFRRVLKAWVVSHPDLVYWQGLDSLCAPFLYLNFNNEALAYACMSAFIPKYLYNFFLKDNSHVIQE YLTVFSQMIAFHDPELSNHLNEIGFIPDLYAIPWFLTMFTHVFPLHKIFHLWDTLLLGNSSFPFCIGVAI LQQLRDRLLANGFNECILLFSDLPEIDIERCVRESINLFCWTPKSATYRQHAQPPKPSSDSSGGRSSAPY FSAECPDPPKTDLSRESIPLNDLKSEVSPRISAEDLIDLCELTVTGHFKTPSKKTKSSKPKLLVVDIRNS EDFIRGHISGSINIPFSAAFTAEGELTQGPYTAMLQNFKGKVIVIVGHVAKHTAEFAAHLVKMKYPRICI LDGGINKIKPTGLLTIPSPQI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290768
ORF Size	2163 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290768.1, NP_001277697.1
RefSeq Size	5410 bp
RefSeq ORF	2166 bp
Locus ID	93627
Protein Families	Druggable Genome, Protein Kinase
MW	81.8 kDa
Gene Summary	This gene encodes a protein that contains a protein kinase domain, a Rhodanase-like domain and the Tre-2/Bub2/Cdc16 (TBC) domain. The encoded protein is thought to play a role in actin organization, cell growth and cell proliferation by regulating the mammalian target of the rapamycin (mTOR) signaling pathway. This protein may also be involved in the transcriptional regulation of the components of the mTOR complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.