XPB (ERCC3) (NM_001303416) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BTF2; GTF2H; RAD25; Ssl2; TFIIH; TTD2; XPB
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239397 representing NM_001303416 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCTGAAGGACGACCACACCTCCAGGCCCCTCTGGGTGGCTCCCGATGGCCATATCTTCTTGGAAG CCTTCTCTCCAGTTTACAAATATGCCCAAGACTTCTTGGTGGCTATTGCAGAGCCAGTGTGCCGACCAAC CCATGTGCATGAGTACAAACTAACTGCCTACTCCTTGTATGCAGCTGTCAGCGTTGGGCTGCAAACCAGT GACATCACCGAGTACCTCAGGAAGCTCAGCAAGACTGGAGTCCCTGATGGAATTATGCAGTTTATTAAGT TGTGTACTGTCAGCTATGGAAAAGTCAAGCTGGTCTTGAAGCACAACAGATACTTCGTTGAAAGTTGCCA CCCTGATGTAATCCAGCATCTTCTCCAGGACCCCGTGATCCGAGAATGCCGCTTAAGAAACTCTGAAGGG GAGGCCACTGAGCTCATCACAGAGACTTTCACAAGCAAATCTGCCATTTCTAAGACTGCTGAAAGCAGTG GTGGGCCCTCCACTTCCCGAGTGACAGATCCACAGGGTAAATCTGACATCCCCATGGACCTGTTTGACTT CTATGAGCAAATGGACAAGGATGAAGAAGAAGAAGAAGAGACACAGACAGTGTCTTTTGAAGTCAAGCAG GAAATGATTGAGGAACTCCAGAAACGTTGCATCCACCTGGAGTACCCTCTGTTGGCAGAATATGACTTCC GGAATGATTCTGTCAACCCTGATATCAACATTGACCTAAAGCCCACAGCTGTCCTCAGACCCTATCAGGA GAAGAGCTTGCGAAAGATGTTTGGAAACGGGCGTGCACGTTCGGGGGTCATTGTTCTTCCCTGCGGTGCT GGAAAGTCCCTGGTTGGTGTGACTGCTGCATGCACTGTCAGAAAACGCTGTCTGGTGCTGGGCAACTCAG CTGTTTCTGTGGAGCAGTGGAAAGCCCAGTTCAAGATGTGGTCCACCATTGACGACAGCCAGATCTGCCG GTTCACCTCCGATGCCAAGGACAAGCCCATCGGCTGCTCCGTTGCCATTAGCACCTACTCCATGCTGGGC CACACCACCAAAAGGTCCTGGGAGGCCGAGCGAGTCATGGAGTGGCTCAAGACCCAGGAGTGGGGCCTCA TGATCCTGGATGAAGTGCACACCATACCAGCCAAGATGTTCCGAAGGGTGCTCACCATCGTGCAGGCCCA CTGTAAGCTGGGTTTGACTGCGACCCTCGTCCGCGAAGATGACAAAATTGTGGATTTAAATTTTCTGATT GGGCCTAAGCTCTACGAAGCCAACTGGATGGAGCTGCAGAATAATGGCTACATCGCCAAAGTCCAGTGTG CTGAGGTCTGGTGCCCTATGTCTCCTGAATTTTACCGGGAATATGTGGCAATCAAAACCAAGAAACGAAT CTTGCTGTACACCATGAACCCCAACAAATTTAGAGCTTGCCAGTTTCTGATCAAGTTTCATGAAAGGAGG AATGACAAGATTATTGTCTTTGCTGACAATGTGTTTGCCCTAAAGGAATATGCCATTCGACTGAACAAAC CCTATATCTACGGACCTACGTCTCAGGGGGAAAGGATGCAAATTCTCCAGAATTTCAAGCACAACCCCAA AATTAACACCATCTTCATATCCAAGGTAGGTGACACTTCGTTTGATCTGCCGGAAGCAAATGTCCTCATT CAGATCTCATCCCATGGTGGCTCCAGGCGTCAGGAAGCCCAAAGGCTAGGGCGGGTGCTTCGAGCTAAAA AAGGGATGGTTGCAGAAGAGTACAATGCCTTTTTCTACTCACTGGTATCCCAGGACACACAGGAAATGGC TTACTCAACCAAGCGGCAGAGATTCTTGGTAGATCAAGGTTATAGCTTCAAGGTGATCACGAAACTCGCT GGCATGGAGGAGGAAGACTTGGCGTTTTCGACAAAAGAAGAGCAACAGCAGCTCTTACAGAAAGTCCTGG CAGCCACTGACCTGGATGCCGAGGAGGAGGTGGTGGCTGGGGAATTTGGCTCCAGATCCAGCCAGGCATC TCGGCGCTTTGGCACCATGAGTTCTATGTCTGGGGCCGACGACACTGTGTACATGGAGTACCACTCATCG CGGAGCAAGGCGCCCAGCAAACATGTACACCCGCTCTTCAAGCGCTTTAGGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239397 representing NM_001303416 Red=Cloning site Green=Tags(s) MPLKDDHTSRPLWVAPDGHIFLEAFSPVYKYAQDFLVAIAEPVCRPTHVHEYKLTAYSLYAAVSVGLQTS DITEYLRKLSKTGVPDGIMQFIKLCTVSYGKVKLVLKHNRYFVESCHPDVIQHLLQDPVIRECRLRNSEG EATELITETFTSKSAISKTAESSGGPSTSRVTDPQGKSDIPMDLFDFYEQMDKDEEEEEETQTVSFEVKQ EMIEELQKRCIHLEYPLLAEYDFRNDSVNPDINIDLKPTAVLRPYQEKSLRKMFGNGRARSGVIVLPCGA GKSLVGVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLG HTTKRSWEAERVMEWLKTQEWGLMILDEVHTIPAKMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLI GPKLYEANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERR NDKIIVFADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLI QISSHGGSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLA GMEEEDLAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSSQASRRFGTMSSMSGADDTVYMEYHSS RSKAPSKHVHPLFKRFRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001303416
ORF Size	2154 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001303416.2
RefSeq Size	2865 bp
RefSeq ORF	2157 bp
Locus ID	2071
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Nucleotide excision repair
MW	82.4 kDa
Gene Summary	This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

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