NR2C2 (NM_001291694) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	TAK1; TR4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239031 representing NM_001291694 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCAGCCCCTCCCCACGCATCCAGATAATCTCCACCGACTCTGCTGTAGCCTCACCTCAGCGCATTC AGATTGTCACAGACCAGCAGACAGGACAGAAAATCCAGATAGTCACCGCAGTGGACGCCTCCGGATCCCC CAAACAGCAGTTCATCCTGACCAGCCCAGATGGAGCTGGAACTGGGAAGGTGATCCTGGCTTCCCCAGAG ACATCCAGCGCCAAGCAACTCATATTCACCACCTCAGACAACCTCGTCCCTGGCAGGATCCAGATTGTCA CGGATTCTGCCTCTGTGGAGCGTTTACTGGGGAAGACGGACGTCCAGCGGCCCCAGGTGGTAGAGTACTG TGTGGTCTGTGGCGACAAAGCCTCCGGCCGTCACTATGGGGCTGTCAGTTGTGAAGGTTGCAAAGGTTTC TTCAAAAGGAGTGTGAGGAAAAATTTGACCTACAGCTGCCGGAGCAACCAAGACTGCATCATCAATAAAC ATCACCGGAACCGCTGTCAGTTTTGCCGGCTGAAAAAATGCTTAGAGATGGGCATGAAAATGGAATCTGT GCAGAGTGAACGGAAGCCCTTCGATGTGCAACGGGAGAAACCAAGCAATTGTGCTGCTTCAACTGAGAAA ATCTATATCCGGAAAGACCTGAGAAGTCCCCTGATAGCTACTCCCACGTTTGTGGCAGACAAAGATGGAG CAAGACAAACAGGTCTTCTTGATCCAGGGATGCTTGTGAACATCCAGCAGCCTTTGATACGTGAGGATGG TACAGTTCTCCTGGCCACGGATTCTAAGGCTGAAACAAGCCAGGGAGCTCTGGGCACACTGGCAAATGTA GTGACCTCCCTTGCCAACCTAAGTGAATCTTTGAACAACGGTGACACTTCAGAAATCCAGCCAGAGGACC AGTCTGCAAGTGAGATAACTCGGGCATTTGATACCTTAGCTAAAGCACTTAATACCACAGACAGCTCCTC TTCTCCAAGCTTGGCAGATGGGATAGACACCAGTGGAGGAGGGAGCATCCACGTCATCAGCAGAGACCAG TCGACACCCATCATTGAGGTTGAAGGCCCCCTCCTTTCAGACACACACGTCACATTTAAGCTAACAATGC CCAGTCCAATGCCAGAGTACCTCAACGTGCACTACATCTGTGAGTCTGCATCCCGTCTGCTTTTCCTCTC AATGCACTGGGCTCGGTCAATCCCAGCCTTTCAGGCACTTGGGCAGGACTGCAACACCAGCCTTGTGCGG GCCTGCTGGAATGAGCTCTTCACCCTCGGCCTGGCCCAGTGTGCCCAGGTCATGAGTCTCTCCACCATCC TGGCTGCCATTGTCAACCACCTGCAGAACAGCATCCAGGAAGATAAACTTTCTGGTGACCGGATAAAGCA AGTCATGGAGCACATCTGGAAGCTGCAGGAGTTCTGTAACAGCATGGCGAAGCTGGATATAGATGGCTAT GAGTATGCATACCTTAAAGCTATAGTTCTCTTTAGCCCCGATCATCCAGGTTTGACCAGCACAAGCCAGA TTGAAAAATTCCAAGAAAAGGCACAGATGGAGTTGCAGGACTATGTTCAGAAAACCTACTCAGAAGACAC CTACCGATTGGCCCGGATCCTCGTTCGCCTGCCGGCACTCAGGCTGATGAGCTCCAACATAACAGAAGAA CTTTTTTTTACTGGTCTCATTGGCAATGTTTCGATAGACAGCATAATCCCCTACATCCTCAAGATGGAGA CAGCAGAGTATAATGGCCAGATCACCGGAGCCAGTCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239031 representing NM_001291694 Red=Cloning site Green=Tags(s) MTSPSPRIQIISTDSAVASPQRIQIVTDQQTGQKIQIVTAVDASGSPKQQFILTSPDGAGTGKVILASPE TSSAKQLIFTTSDNLVPGRIQIVTDSASVERLLGKTDVQRPQVVEYCVVCGDKASGRHYGAVSCEGCKGF FKRSVRKNLTYSCRSNQDCIINKHHRNRCQFCRLKKCLEMGMKMESVQSERKPFDVQREKPSNCAASTEK IYIRKDLRSPLIATPTFVADKDGARQTGLLDPGMLVNIQQPLIREDGTVLLATDSKAETSQGALGTLANV VTSLANLSESLNNGDTSEIQPEDQSASEITRAFDTLAKALNTTDSSSSPSLADGIDTSGGGSIHVISRDQ STPIIEVEGPLLSDTHVTFKLTMPSPMPEYLNVHYICESASRLLFLSMHWARSIPAFQALGQDCNTSLVR ACWNELFTLGLAQCAQVMSLSTILAAIVNHLQNSIQEDKLSGDRIKQVMEHIWKLQEFCNSMAKLDIDGY EYAYLKAIVLFSPDHPGLTSTSQIEKFQEKAQMELQDYVQKTYSEDTYRLARILVRLPALRLMSSNITEE LFFTGLIGNVSIDSIIPYILKMETAEYNGQITGASL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291694
ORF Size	1788 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291694.2
RefSeq Size	8428 bp
RefSeq ORF	1791 bp
Locus ID	7182
UniProt ID	P49116
Protein Families	Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
MW	65.9 kDa
Gene Summary	This gene encodes a protein that belongs to the nuclear hormone receptor family. Members of this family act as ligand-activated transcription factors and function in many biological processes such as development, cellular differentiation and homeostasis. The activated receptor/ligand complex is translocated to the nucleus where it binds to hormone response elements of target genes. The protein encoded by this gene plays a role in protecting cells from oxidative stress and damage induced by ionizing radiation. The lack of a similar gene in mouse results in growth retardation, severe spinal curvature, subfertility, premature aging, and prostatic intraepithelial neoplasia (PIN) development. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2014]

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