CYP39A1 (NM_001278739) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237273 representing NM_001278739 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTTTAATAAAAGTTTGTTTTCCACAAACAAGAAAAAAATCAAGGAGTTCCATCAGTATTTTCAAG TTTATGATGAAGATTTTGAGTATGGGTCCCAGTTGCCAGAGTGTCTTCTAAGAAACTGGTCAAAATCCAA AAAGTGGTTCCTGGAACTGTTTGAGAAAAACATTCCAGATATAAAAGCATGTAAATCTGCAAAAGATAAT TCCATGACATTATTGCAAGCTACGCTGGATATTGTAGAGACGGAAACAAGTAAGGAAAACTCACCCAATT ATGGGCTCTTACTGCTTTGGGCTTCTCTGTCTAATGCTGTTCCTGTTGCATTTTGGACACTTGCATACGT CCTTTCTCATCCTGATATCCACAAGGCCATTATGGAAGGCATATCTTCTGTGTTTGGCAAAGCAGGCAAA GATAAGATTAAAGTGTCTGAGGATGACCTGGAGAATCTCCTTCTAATTAAATGGTGTGTTTTGGAAACCA TTCGTTTAAAAGCTCCTGGTGTCATTACTAGAAAAGTGGTGAAGCCTGTGGAAATTTTGAATTACATCAT TCCTTCTGGTGACTTGTTGATGTTGTCTCCATTTTGGCTGCATAGAAATCCAAAGTATTTTCCTGAGCCT GAATTGTTCAAACCTGAACGTTGGAAAAAGGCAAATTTAGAGAAGCACTCTTTCTTGGACTGCTTCATGG CATTTGGAAGCGGGAAGTTCCAGTGTCCTGCAAGGTGGTTTGCTCTGTTAGAGGTTCAGATGTGTATTAT TTTAATACTTTATAAATATGACTGTAGTCTTCTGGACCCATTACCCAAACAGAGTTATCTCCATTTGGTG GGTGTCCCCCAGCCGGAAGGGCAATGCCGAATTGAATATAAACAAAGAATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237273 representing NM_001278739 Red=Cloning site Green=Tags(s) MLFNKSLFSTNKKKIKEFHQYFQVYDEDFEYGSQLPECLLRNWSKSKKWFLELFEKNIPDIKACKSAKDN SMTLLQATLDIVETETSKENSPNYGLLLLWASLSNAVPVAFWTLAYVLSHPDIHKAIMEGISSVFGKAGK DKIKVSEDDLENLLLIKWCVLETIRLKAPGVITRKVVKPVEILNYIIPSGDLLMLSPFWLHRNPKYFPEP ELFKPERWKKANLEKHSFLDCFMAFGSGKFQCPARWFALLEVQMCIILILYKYDCSLLDPLPKQSYLHLV GVPQPEGQCRIEYKQRI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278739
ORF Size	891 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278739.1, NP_001265668.1
RefSeq Size	2269 bp
RefSeq ORF	894 bp
Locus ID	51302
UniProt ID	Q9NYL5
Protein Families	Druggable Genome, P450, Transmembrane
Protein Pathways	Primary bile acid biosynthesis
MW	34.9 kDa
Gene Summary	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is involved in the conversion of cholesterol to bile acids. Its substrates include the oxysterols 25-hydroxycholesterol, 27-hydroxycholesterol and 24-hydroxycholesterol. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

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