THBS3 (NM_001252608) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	TSP3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234942 representing NM_001252608 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACGCAGGAACTTCGGGGGGCCCTGGCTCTTCTCCTCCTTTGCTTTTTCACATCTGCCAGTCAGG ATCTGCAGGTAATTGACCTGCTGACTGTGGGCGAGTCTCGGCAGATGGTAGCTGTGGCAGAGAAGATCCG GACAGCCTTGCTCACTGCTGGGGACATCTACCTCTTATCCACCTTCCGCCTGCCCCCCAAGCAGGGTGGT GTCCTCTTTGGCCTCTATTCTCGCCAAGACAACACTCGATGGCTGGAGGCCTCTGTTGTAGGCAAGATCA ACAAAGTGACCAATGCACTGCACTCCATTCTAGGGGAGCAGACCAAGGCGCTGGTCACCCAACTCACCCT CTTCAACCAGATCCTGGTGGAGCTGCGGGATGATATACGAGACCAGGTGAAGGAAATGTCCCTGATCCGA AACACCATTATGGAGTGTCAGGTGTGCGGCTTCCATGAGCAGCGTTCCCACTGCAGCCCCAATCCCTGCT TCCGAGGTGTGGACTGCATGGAAGTGTACGAGTACCCAGGCTACCGCTGTGGGCCCTGCCCCCCTGGCCT GCAGGGCAACGGCACCCACTGCAGTGACATCAATGAGTGTGCTCACGCTGACCCCTGTTTCCCGGGCTCC AGCTGCATCAACACCATGCCCGGCTTCCACTGTGAGGCCTGTCCTCGAGGGTACAAGGGCACACAGGTGT CTGGTGTGGGCATTGACTATGCCCGGGCCAGCAAACAGGTCTGCAATGACATCGATGAATGCAACGATGG CAACAATGGTGGCTGTGACCCAAACTCCATCTGCACCAACACTGTGGGCTCTTTCAAGTGTGGTCCCTGC CGCCTGGGTTTCCTGGGCAACCAGAGCCAGGGCTGCCTCCCAGCCCGGACCTGCCACAGCCCAGCCCACA GCCCCTGCCACATCCATGCTCACTGTCTCTTTGAACGCAATGGTGCAGTGTCCTGCCAGTGTAACGTGGG CTGGGCTGGGAATGGGAACGTGTGTGGGACTGACACAGACATCGATGGCTACCCAGACCAAGCACTGCCC TGCATGGACAACAACAAACACTGCAAACAGGACAACTGCCTTTTGACACCCAACTCTGGGCAGGAAGATG CTGATAATGATGGTGTGGGGGACCAGTGTGATGATGATGCTGATGGGGATGGGATCAAGAATGTTGAGGA CAACTGCCGGCTGTTCCCCAACAAAGACCAGCAGAACTCAGATACAGATTCATTTGGTGATGCCTGTGAC AATTGCCCCAACGTTCCCAACAATGACCAGAAGGACACAGATGGCAATGGGGAAGGAGATGCCTGTGACA ACGACGTGGATGGGGATGGCATCCCCAATGGATTGGACAATTGCCCTAAAGTCCCCAACCCACTACAGAC AGACAGGGATGAGGACGGGGTGGGAGATGCTTGCGACAGCTGCCCTGAAATGAGCAATCCTACCCAGACA GATGCAGACAGCGACCTGGTGGGGGATGTCTGTGATACTAATGAAGACAGCGATGGGGATGGGCATCAGG ACACCAAGGACAACTGCCCACAGCTGCCAAATAGCTCCCAGCTGGACTCTGATAACGATGGACTTGGAGA TGAGTGTGATGGGGATGATGACAATGATGGCATCCCAGATTATGTGCCTCCTGGTCCCGATAACTGCCGC CTGGTACCCAATCCCAATCAGAAGGACTCAGATGGCAATGGCGTTGGTGATGTGTGTGAGGATGACTTTG ACAATGATGCTGTGGTCGACCCCCTGGATGTGTGTCCTGAAAGTGCAGAGGTAACGCTTACGGATTTTCG GGCCTATCAGACCGTCGTCCTGGATCCTGAGGGTGATGCTCAGATTGACCCAAACTGGGTTGTGCTCAAC CAGGGCATGGAAATCGTTCAGACCATGAACAGTGACCCTGGCTTGGCAGTTGGATACACGGCCTTCAATG GTGTGGACTTTGAAGGCACCTTCCATGTGAACACAGTGACTGATGATGACTACGCAGGCTTTCTCTTCAG TTATCAAGACAGTGGCCGCTTCTACGTAGTCATGTGGAAGCAGACCGAGCAGACCTACTGGCAGGCTACA CCCTTCCGGGCGGTTGCCCAGCCCGGGCTGCAGCTCAAGGCAGTGACATCAGTGTCTGGCCCAGGTGAGC ACCTCCGAAATGCCCTGTGGCATACTGGCCACACCCCTGATCAGGTACGACTGCTGTGGACAGACCCACG AAATGTGGGCTGGCGGGACAAGACCTCCTATCGCTGGCAGCTTCTGCACCGGCCTCAAGTTGGCTACATT CGGGTGAAGCTCTATGAGGGACCCCAGCTTGTGGCGGATTCTGGGGTGATCATTGACACATCCATGCGAG GGGGGCGTCTTGGTGTATTCTGCTTCTCCCAAGAAAACATAATTTGGTCCAATCTCCAGTATCGATGCAA TGACACAGTGCCTGAGGACTTTGAGCCATTCCGGAGGCAGCTGCTCCAGGGAAGGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234942 representing NM_001252608 Red=Cloning site Green=Tags(s) METQELRGALALLLLCFFTSASQDLQVIDLLTVGESRQMVAVAEKIRTALLTAGDIYLLSTFRLPPKQGG VLFGLYSRQDNTRWLEASVVGKINKVTNALHSILGEQTKALVTQLTLFNQILVELRDDIRDQVKEMSLIR NTIMECQVCGFHEQRSHCSPNPCFRGVDCMEVYEYPGYRCGPCPPGLQGNGTHCSDINECAHADPCFPGS SCINTMPGFHCEACPRGYKGTQVSGVGIDYARASKQVCNDIDECNDGNNGGCDPNSICTNTVGSFKCGPC RLGFLGNQSQGCLPARTCHSPAHSPCHIHAHCLFERNGAVSCQCNVGWAGNGNVCGTDTDIDGYPDQALP CMDNNKHCKQDNCLLTPNSGQEDADNDGVGDQCDDDADGDGIKNVEDNCRLFPNKDQQNSDTDSFGDACD NCPNVPNNDQKDTDGNGEGDACDNDVDGDGIPNGLDNCPKVPNPLQTDRDEDGVGDACDSCPEMSNPTQT DADSDLVGDVCDTNEDSDGDGHQDTKDNCPQLPNSSQLDSDNDGLGDECDGDDDNDGIPDYVPPGPDNCR LVPNPNQKDSDGNGVGDVCEDDFDNDAVVDPLDVCPESAEVTLTDFRAYQTVVLDPEGDAQIDPNWVVLN QGMEIVQTMNSDPGLAVGYTAFNGVDFEGTFHVNTVTDDDYAGFLFSYQDSGRFYVVMWKQTEQTYWQAT PFRAVAQPGLQLKAVTSVSGPGEHLRNALWHTGHTPDQVRLLWTDPRNVGWRDKTSYRWQLLHRPQVGYI RVKLYEGPQLVADSGVIIDTSMRGGRLGVFCFSQENIIWSNLQYRCNDTVPEDFEPFRRQLLQGRV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252608
ORF Size	2508 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252608.2
RefSeq Size	2891 bp
RefSeq ORF	2511 bp
Locus ID	7059
UniProt ID	P49746
Protein Families	Druggable Genome
Protein Pathways	ECM-receptor interaction, Focal adhesion, TGF-beta signaling pathway
MW	91.7 kDa
Gene Summary	The protein encoded by this gene belongs to the thrombospondin family. Thrombospondin family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentameric molecule linked by a single disulfide bond. This gene shares a common promoter with metaxin 1. Alternate splicing results in coding and non-coding transcript variants. [provided by RefSeq, Nov 2011]

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