MAP3K13 (NM_001242317) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	LZK; MEKK13; MLK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234846 representing NM_001242317 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATTGTGGCATTCAAATCCTAGCACTCTGGGAGAGGGGTGTTTGTACTCAGGCCCCATGTTATTGTA TTATCATGGAATACTGTGCCCATGGACAACTCTACGAGGTCTTACGAGCTGGCAGGAAGATCACACCTCG ATTGCTAGTAGACTGGTCCACAGGAATTGCAAGTGGAATGAATTATTTGCACCTCCATAAAATTATTCAT CGTGATCTCAAATCACCTAATGTTTTAGTGACCCACACAGATGCGGTAAAAATTTCAGATTTTGGTACAT CTAAGGAACTCAGTGACAAAAGTACCAAGATGTCATTTGCTGGCACGGTCGCATGGATGGCGCCAGAGGT GATACGGAATGAACCTGTCTCTGAAAAAGTTGATATATGGTCTTTTGGAGTGGTGCTTTGGGAGCTGCTG ACAGGAGAGATCCCTTACAAAGATGTAGATTCTTCAGCCATTATCTGGGGTGTTGGAAGCAACAGCCTCC ACCTTCCAGTTCCTTCCACTTGCCCTGATGGATTCAAAATCCTTATGAAACAGACGTGGCAGAGTAAACC TCGAAACCGACCTTCTTTTCGGCAGACACTCATGCATTTAGACATTGCCTCTGCAGATGTACTTGCCACC CCACAAGAAACTTACTTCAAGTCTCAGGCTGAATGGAGAGAAGAAGTGAAAAAACATTTTGAGAAGATCA AAAGTGAAGGAACTTGTATACACCGGTTAGATGAAGAACTGATTCGAAGGCGCAGAGAAGAGCTCAGGCA TGCGCTGGATATTCGTGAACACTATGAGCGGAAGCTTGAGCGGGCGAATAATTTATACATGGAATTGAGT GCCATCATGCTGCAGCTAGAAATGCGGGAGAAGGAGCTCATTAAGCGTGAGCAAGCAGTGGAAAAGAAGT ATCCTGGGACCTACAAACGACACCCTGTTCGTCCTATCATCCATCCCAATGCCATGGAGAAACTCATGAA AAGGAAAGGAGTGCCTCACAAATCTGGGATGCAGACCAAACGGCCAGACTTGTTGAGATCAGAAGGGATC CCCACCACAGAAGTGGCTCCCACTGCATCCCCTTTGTCCGGAAGTCCCAAAATGTCCACTTCTAGCAGCA AGAGCCGATATCGAAGCAAACCACGCCACCGCCGAGGGAATAGCAGAGGCAGCCATAGTGACTTTGCCGC AATCTTGAAAAACCAGCCAGCCCAGGAAAATTCACCCCATCCCACTTACCTGCACCAAGCTCAATCCCAA TACCCTTCTCTTCATCACCATAATTCTCTGCAGCAGCAATACCAGCAGCCCCCTCCTGCCATGTCCCAGA GTCACCATCCCAGACTCAATATGCACGGACAGGACATAGCAACCTGCGCCAACAACCTGAGGTATTTCGG CCCAGCAGCAGCCCTGCGGAGCCCACTCAGCAACCATGCTCAGAGACAGCTGCCCGGCTCGAGCCCTGAC CTCATCTCCACAGCCATGGCTGCAGACTGCTGGAGAAGTTCTGAGCCTGACAAGGGCCAAGCTGGTCCCT GGGGCTGTTGCCAGGCTGACGCTTATGACCCCTGCCTTCAGTGCAGGCCAGAACAGTATGGGTCCTTAGA CATACCCTCTGCTGAGCCAGTGGGGAGGAGCCCTGACCTTTCCAAGTCACCAGCACATAATCCTCTCTTG GAAAACGCCCAGAGTTCTGAGAAAACGGAAGAAAATGAATTCAGCGGCTGTAGGTCTGAGTCATCCCTCG GCACCTCTCATCTCGGCACCCCTCCAGCGCTACCTCGAAAAACAAGGCCTCTGCAGAAGAGTGGAGATGA CTCCTCAGAAGAGGAAGAAGGGGAAGTAGATAGTGAAGTTGAATTTCCACGAAGACAGAGGCCCCATCGC TGTATCAGCAGCTGCCAGTCATATTCAACCTTTAGCTCTGAGAATTTCTCTGTGTCTGATGGAGAAGAGG GAAATACCAGTGACCACTCAAACAGTCCTGATGAGTTAGCTGATAAACTTGAAGACCGCTTGGCAGAGAA GCTAGACGACCTGCTGTCCCAGACGCCAGAGATTCCCATTGACATATCCTCACACTCGGATGGGCTCTCT GACAAGGAGTGTGCCGTGCGCCGTGTGAAGACTCAGATGTCTCTGGGCAAGCTGTGTGTGGAGGAACGTG GCTATGAGAACCCCATGCAGTTTGAAGAATCGGACTGTGACTCTTCAGATGGGGAGTGTTCTGATGCCAC AGTTAGGACCAATAAACACTACAGCTCTGCTACCTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234846 representing NM_001242317 Red=Cloning site Green=Tags(s) MYCGIQILALWERGVCTQAPCYCIIMEYCAHGQLYEVLRAGRKITPRLLVDWSTGIASGMNYLHLHKIIH RDLKSPNVLVTHTDAVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELL TGEIPYKDVDSSAIIWGVGSNSLHLPVPSTCPDGFKILMKQTWQSKPRNRPSFRQTLMHLDIASADVLAT PQETYFKSQAEWREEVKKHFEKIKSEGTCIHRLDEELIRRRREELRHALDIREHYERKLERANNLYMELS AIMLQLEMREKELIKREQAVEKKYPGTYKRHPVRPIIHPNAMEKLMKRKGVPHKSGMQTKRPDLLRSEGI PTTEVAPTASPLSGSPKMSTSSSKSRYRSKPRHRRGNSRGSHSDFAAILKNQPAQENSPHPTYLHQAQSQ YPSLHHHNSLQQQYQQPPPAMSQSHHPRLNMHGQDIATCANNLRYFGPAAALRSPLSNHAQRQLPGSSPD LISTAMAADCWRSSEPDKGQAGPWGCCQADAYDPCLQCRPEQYGSLDIPSAEPVGRSPDLSKSPAHNPLL ENAQSSEKTEENEFSGCRSESSLGTSHLGTPPALPRKTRPLQKSGDDSSEEEEGEVDSEVEFPRRQRPHR CISSCQSYSTFSSENFSVSDGEEGNTSDHSNSPDELADKLEDRLAEKLDDLLSQTPEIPIDISSHSDGLS DKECAVRRVKTQMSLGKLCVEERGYENPMQFEESDCDSSDGECSDATVRTNKHYSSATW TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242317
ORF Size	2277 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242317.2
RefSeq Size	9094 bp
RefSeq ORF	2280 bp
Locus ID	9175
UniProt ID	O43283
Protein Families	Druggable Genome, Protein Kinase, Transcription Factors
Protein Pathways	MAPK signaling pathway
MW	85.9 kDa
Gene Summary	The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase contains a dual leucine-zipper motif, and has been shown to form dimers/oligomers through its leucine-zipper motif. This kinase can phosphorylate and activate MAPK8/JNK, MAP2K7/MKK7, which suggests a role in the JNK signaling pathway. [provided by RefSeq, Jul 2008]

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