EPB41L1 (NM_001258330) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4.1N; MRD11
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234742 representing NM_001258330 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTTTCTTGGGGAGGATCAATGAGGTAGAACCTGCAAAGGGCTTAGCAGAGAGCCTAGCACCTACTG AGCGCTCAGTAAAGAGCCTAGACATGGAGGAGAAGGACTACAGTGAGGCCGATGGCCTTTCGGAGAGGAC CACGCCCAGCAAGGCCCAGAAATCGCCCCAGAAGATTGCCAAGAAATACAAGAGTGCCATCTGCCGGGTC ACTCTGCTTGATGCCTCGGAGTATGAGTGTGAGGTGGAGAAACATGGCCGGGGCCAGGTGCTGTTTGACC TGGTCTGTGAACACCTCAACCTCCTAGAGAAGGACTACTTCGGCCTGACCTTCTGTGATGCTGACAGCCA GAAGAACTGGCTGGACCCCTCCAAGGAGATCAAGAAGCAGATCCGGAGTAGCCCCTGGAATTTTGCCTTC ACAGTCAAGTTCTACCCGCCTGATCCTGCCCAGCTGACAGAAGACATCACAAGATACTACCTGTGCCTGC AGCTGCGGGCAGACATCATCACGGGCCGGCTGCCATGCTCCTTTGTCACGCATGCCCTACTGGGCTCCTA CGCTGTGCAGGCTGAGCTGGGTGACTATGATGCTGAGGAGCATGTGGGCAACTATGTCAGCGAGCTCCGC TTCGCCCCTAACCAGACCCGGGAGCTGGAGGAGAGGATCATGGAGCTGCATAAGACATATAGGGGGATGA CCCCGGGAGAAGCAGAAATCCACTTCTTAGAGAATGCCAAGAAGCTTTCCATGTACGGAGTAGACCTGCA CCATGCCAAGGACTCTGAGGGCATCGACATCATGTTAGGCGTTTGTGCCAATGGCCTGCTCATCTACCGG GACCGGCTGAGAATCAACCGCTTTGCCTGGCCCAAGATCCTCAAGATCTCCTACAAGAGGAGTAACTTCT ATATCAAGATCCGGCCTGGGGAGTATGAGCAATTTGAGAGCACAATTGGCTTTAAGCTCCCAAACCACCG GTCAGCCAAGAGACTGTGGAAGGTCTGCATCGAGCATCATACATTCTTCCGGCTGGTGTCCCCTGAGCCC CCACCCAAGGGCTTCCTGGTGATGGGCTCCAAGTTCCGGTACAGTGGGAGGACCCAGGCACAGACTCGCC AGGCCAGCGCCCTCATTGACCGGCCTGCACCCTTCTTTGAGCGTTCTTCCAGCAAACGGTACACCATGTC CCGCAGCCTTGATGGAGCAGAGTTCTCCCGCCCAGCCTCGGTCAGCGAGAACCATGATGCAGGGCCTGAC GGTGACAAGCGGGATGAGGATGGCGAGTCTGGGGGGCAACGGTCAGAGGCTGAGGAGGGAGAGGTCAGGA CTCCAACCAAGATCAAGGAGCTAAAGTTCTTAGACAAGCCAGAAGATGTCTTGCTGAAGCACCAGGCCAG CATCAATGAGCTCAAAAGGACCCTGAAGGAGCCCAACAGCAAACTCATCCACCGGGATCGAGACTGGGAA CGGGAGCGCAGGCTGCCCTCCTCCCCCGCCTCCCCCTCCCCCAAGGGCACCCCTGAGAAAGCCAATGAGC CCGTGAAAACAGAAACCATGACTGTCAGCAGTCTGGCCATTAGAAAGAAGATTGAGCCGGAGGCCGTACT GCAGACCAGAGTCTCCGCTATGGATAACACCCAGCAGGTTGATGGGAGTGCCTCAGTGGGGAGGGAGTTC ATAGCAACCACTCCCTCCATCACCACGGAGACCATATCGACCACCATGGAGAACAGTCTCAAGTCCGGGA AGGGGGCAGCTGCCATGATCCCAGGCCCACAGACGGTGGCCACGGAAATCCGTTCTCTTTCTCCGATCAT CGGGAAAGATGTCCTCACCAGCACCTACGGCGCCACTGCGGAAACCCTCTCAACCTCCACCACCACCCAT GTCACCAAAACTGTGAAAGGAGGGTTTTCTGAGACAAGGATCGAGAAGCGAATCATCATTACTGGGGATG AAGATGTCGATCAAGACCAGGCCCTGGCTTTGGCCATCAAGGAGGCCAAACTGCAGCATCCTGATATGCT GGTAACCAAAGCTGTCGTATACAGAGAAACAGACCCATCCCCAGAGGAGAGGGACAAGAAGCCACAGGAA TCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234742 representing NM_001258330 Red=Cloning site Green=Tags(s) MVFLGRINEVEPAKGLAESLAPTERSVKSLDMEEKDYSEADGLSERTTPSKAQKSPQKIAKKYKSAICRV TLLDASEYECEVEKHGRGQVLFDLVCEHLNLLEKDYFGLTFCDADSQKNWLDPSKEIKKQIRSSPWNFAF TVKFYPPDPAQLTEDITRYYLCLQLRADIITGRLPCSFVTHALLGSYAVQAELGDYDAEEHVGNYVSELR FAPNQTRELEERIMELHKTYRGMTPGEAEIHFLENAKKLSMYGVDLHHAKDSEGIDIMLGVCANGLLIYR DRLRINRFAWPKILKISYKRSNFYIKIRPGEYEQFESTIGFKLPNHRSAKRLWKVCIEHHTFFRLVSPEP PPKGFLVMGSKFRYSGRTQAQTRQASALIDRPAPFFERSSSKRYTMSRSLDGAEFSRPASVSENHDAGPD GDKRDEDGESGGQRSEAEEGEVRTPTKIKELKFLDKPEDVLLKHQASINELKRTLKEPNSKLIHRDRDWE RERRLPSSPASPSPKGTPEKANEPVKTETMTVSSLAIRKKIEPEAVLQTRVSAMDNTQQVDGSASVGREF IATTPSITTETISTTMENSLKSGKGAAAMIPGPQTVATEIRSLSPIIGKDVLTSTYGATAETLSTSTTTH VTKTVKGGFSETRIEKRIIITGDEDVDQDQALALAIKEAKLQHPDMLVTKAVVYRETDPSPEERDKKPQE S TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001258330
ORF Size	2103 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001258330.1, NP_001245259.1
RefSeq Size	5745 bp
RefSeq ORF	2106 bp
Locus ID	2036
UniProt ID	Q9H4G0
Protein Families	Druggable Genome
Protein Pathways	Tight junction
MW	79.3 kDa
Gene Summary	Erythrocyte membrane protein band 4.1 (EPB41) is a multifunctional protein that mediates interactions between the erythrocyte cytoskeleton and the overlying plasma membrane. The encoded protein binds and stabilizes D2 and D3 dopamine receptors at the neuronal plasma membrane. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]

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