Synphilin 1 (SNCAIP) (NM_001242935) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Sph1; SYPH1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234537 representing NM_001242935 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACATATCTTATTCAGTCACATCACTCAAGACGATCCCAGAACTGTGCCGAAGATGTGATACGCAAAA CGAAGACAGATCAGAATGGTCAGTTGGAGTGCGTACGCTGGATGGTGAGCGAAACAGAAGCCATTGCAGA ACTGAGTTGTTCTAAGGATTTTCCAAGCCTTATTCATTACGCAGGTTGCTATGGCCAGGAAAAGATTCTT CTGTGGCTTCTTCAGTTTATGCAAGAACAGGGCATCTCGTTGGATGAAGTAGACCAGGATGGCAACAGTG CCGTTCACGTAGCCTCACAGCATGGCTACCTTGGATGCATACAGACCTTGGTTGAATATGGAGCAAATGT CACCATGCAGAACCACGCTGGGGAAAAGCCCTCCCAGAGCGCCGAGCGGCAGGGGCACACCCTGTGCTCC AGGTACCTGGTGGTGGTGGAGACCTGCATGTCGCTGGCCTCTCAAGTGGTGAAGTTAACCAAGCAGCTAA AGGAACAAACAGTAGAACGTGTCACGCTGCAGAACCAACTCCAACAATTTCTAGAAGCCCAGAAATCAGA GGGCAAGTCACTCCCTTCTTCACCCAGTTCACCATCCTCACCTGCCTCCAGAAAGTCCCAGTGGAAATCT CCAGATGCAGATGATGATTCTGTAGCCAAAAGCAAGCCAGGAGTCCAAGAGGGGATTCAGGTTCTTGGAA GCCTGTCAGCCTCCAGCCGGGCTAGACCCAAAGCAAAAGATGAAGATTCTGATAAAATCTTACGCCAGTT ATTGGGAAAGGAAATCTCAGAAAATGTCTGCACCCAGGAAAAACTGTCCTTGGAATTCCAGGATGCTCAG GCTTCCTCTAGAAATTCTAAAAAGATCCCACTGGAGAAGAGGGAACTGAAGTTAGCCAGGCTGAGACAGC TGATGCAGAGGTCACTGAGTGAGTCTGACACAGACTCCAACAACTCTGAGGACCCCAAGACTACCCCAGT GAGGAAGGCTGACCGACCAAGGCCGCAGCCCATTGTAGAAAGCGTAGAGAGTATGGACAGCGCAGAAAGC CTGCACCTGATGATTAAGAAACACACCTTGGCATCAGGGGGACGCAGGTTTCCTTTCAGCATCAAGGCCT CCAAATCCCTGGATGGCCACAGCCCATCTCCCACCTCAGAGAGCAGCGAACCAGACTTAGAATCCCAGTA TCCAGGCTCAGGGAGTATTCCTCCAAACCAGCCCTCTGGTGACCCTCAGCAGCCCAGCCCTGACAGTACT GCTGCCCAGAAAGTTGCCACAAGTCCCAAGAGTGCCCTCAAGTCTCCATCTTCCAAGCGTAGGACATCTC AGAACTTAAAACTGAGAGTTACCTTTGAGGAGCCTGTGGTGCAGATGGAGCAGCCTAGCCTTGAACTGAA TGGAGAAAAAGACAAAGATAAGGGCAGGACTCTCCAGCGGACCTCCACAAGTAACGAATCGGGGGATCAA CTGAAAAGGCCTTTTGGAGCCTTTCGATCTATCATGGAGACACTAAGTGGCAACCAAAACAATAATAATA ACTACCAGGCAGCCAACCAGCTGAAAACCTCTACATTGCCCTTGACCTCACTTGGGAGGAAGACAGATGC CAAGGGAAACCCTGCCAGCTCCGCTAGCAAAGGAAAGAATAAGGCAGAAATGTACAGCAGCTGCATCAAT CTTTCCTCTAACATGCTGATTGAAGAGCATCTGTGTAACGACACACGGCATAATGACATCAATAGAAAAA TGAAGAAATCCTACAGCATAAAGCACATTGCTGAGCCAGAGTCAAAAGAACTCTTCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234537 representing NM_001242935 Red=Cloning site Green=Tags(s) MTYLIQSHHSRRSQNCAEDVIRKTKTDQNGQLECVRWMVSETEAIAELSCSKDFPSLIHYAGCYGQEKIL LWLLQFMQEQGISLDEVDQDGNSAVHVASQHGYLGCIQTLVEYGANVTMQNHAGEKPSQSAERQGHTLCS RYLVVVETCMSLASQVVKLTKQLKEQTVERVTLQNQLQQFLEAQKSEGKSLPSSPSSPSSPASRKSQWKS PDADDDSVAKSKPGVQEGIQVLGSLSASSRARPKAKDEDSDKILRQLLGKEISENVCTQEKLSLEFQDAQ ASSRNSKKIPLEKRELKLARLRQLMQRSLSESDTDSNNSEDPKTTPVRKADRPRPQPIVESVESMDSAES LHLMIKKHTLASGGRRFPFSIKASKSLDGHSPSPTSESSEPDLESQYPGSGSIPPNQPSGDPQQPSPDST AAQKVATSPKSALKSPSSKRRTSQNLKLRVTFEEPVVQMEQPSLELNGEKDKDKGRTLQRTSTSNESGDQ LKRPFGAFRSIMETLSGNQNNNNNYQAANQLKTSTLPLTSLGRKTDAKGNPASSASKGKNKAEMYSSCIN LSSNMLIEEHLCNDTRHNDINRKMKKSYSIKHIAEPESKELFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242935
ORF Size	1809 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242935.2
RefSeq Size	2588 bp
RefSeq ORF	1812 bp
Locus ID	9627
UniProt ID	Q9Y6H5
Protein Pathways	Parkinson's disease
MW	67.1 kDa
Gene Summary	This gene encodes a protein containing several protein-protein interaction domains, including ankyrin-like repeats, a coiled-coil domain, and an ATP/GTP-binding motif. The encoded protein interacts with alpha-synuclein in neuronal tissue and may play a role in the formation of cytoplasmic inclusions and neurodegeneration. A mutation in this gene has been associated with Parkinson's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

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