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NT5C1B (NT5C1B-RDH14) (NM_001199104) Human Tagged ORF Clone
CAT＃: RC231332
NT5C1B (Myc-DDK-tagged)-Human NT5C1B-RDH14 readthrough (NT5C1B-RDH14), transcript variant 2
ORF Plasmid: tGFP
"NM_001199104" in other vectors (3)
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CNY 4,480.00
CNY 5,230.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AIRP; cN-IB; cN1B; NT5C1B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC231332 representing NM_001199104
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCAAACATCTCTCAAACAGAAAAAGAATGAGCCTGGAATGAGGTCCTCAAAAGAGAGTCTAGAAG CAGAAAAAAGAAAGGAATCTGACAAAACAGGAGTTCGTCTGAGCAATCAGATGAGGCGTGCAGTCAATCC GAATCACTCGCTGAGATGTTGCCCCTTCCAGGGTCACTCGTCGTGTAGACGCTGCCTTTGTGCAGCTGAG GGAACAGCCCTTGGCCCCTGCCACACAATACGTATTTATATTCACATGTGCCTGTTGTGGGAGCAGGGCC AGCAGATCACCATGATGAGGGGATCACAAGAATCATCACTGCGGAAGACAGACTCTCGAGGGTACCTTGT GCGCAGTCAATGGTCTAGAATATCCCGGAGCCCATCCACCAAGGCTCCATCCATAGATGAGCCTAGAAGC AGGAACACCAGTGCTAAGCTCCCCAGCAGCTCCACGAGCTCCCGGACTCCATCCACCTCCCCAAGCCTGC ATGACTCCTCACCGCCGCCGCTGTCCGGGCAGCCCTCGCTCCAGCCACCCGCGTCGCCCCAGCTGCCCCG GTCGCTGGACTCGCGGCCTCCCACGCCCCCAGAGCCCGATCCTGGCTCCCGGCGCAGCACCAAAATGCAA GAGAATCCGGAGGCCTGGGCCCAAGGCATCGTGCGGGAAATCCGCCAGACCCGGGACTCGCAGCCGCTGG AATATTCGCGCACGTCCCCCACCGAGTGGAAGTCCTCCAGCCAGCGCAGGGGGATCTACCCCGCCTCCAC CCAGCTGGACCGCAACTCTCTGTCCGAGCAGCAGCAGCAGCAGCGGGAGGACGAGGACGACTACGAGGCT GCCTACTGGGCATCCATGAGGTCGTTCTACGAGAAGAACCCGAGCTGCTCGCGCCCCTGGCCGCCCAAAC CCAAGAACGCCATCACCATTGCTCTCTCATCCTGCGCGCTCTTCAACATGGTGGACGGCAGGAAAATCTA CGAGCAAGAGGGTCTGGAAAAGTACATGGAGTATCAGCTCACCAATGAGAACGTCATCCTGACCCCGGGC CCGGCGTTCCGCTTCGTCAAGGCACTACAGTATGTCAATGCTAGACTCCGTGATCTATATCCTGATGAAC AGGACTTATTTGATATTGTACTGATGACTAATAACCATGCCCAAGTGGGAGTGCGGCTTATAAACAGCGT CAATCACTACGGCTTACTGATTGACCGCTTCTGTCTGACCGGGGGAAAAGACCCCATTGGCTATTTGAAG GCATATCTTACCAACTTGTATATTGCTGCAGATTCTGAAAAAGTGCAAGAGGCAATACAAGAAGGTATTG CCTCTGCGACAATGTTTGATGGAGCCAAAGACATGGCTTACTGTGACACTCAGCTCCGTGTAGCCTTTGA TGGGGATGCTGTCCTCTTCTCTGATGAGTCTGAACATTTTACCAAGGAGCATGGGCTCGACAAATTCTTC CAGTATGATACATTATGTGAAAGTAAGCCTCTTGCTCAGGGTCCCCTAAAAGGCTTTCTGGAAGATTTAG GCAGACTGCAAAAGAAGTTCTATGCCAAAAATGAACGGTTACTTTGTCCTATCAGGACCTACCTGGTTAC AGCTAGGAGTGCAGCCAGTTCAGGCGCCCGTGTGCTGAAGACCCTTCGACGCTGGGGTCTAGAGATAGAC GAAGCTCTTTTCCTTGCTGGAGCCCCCAAAAGTCCCATCTTGGTGAAGATCCGGCCCCACATCTTCTTTG ATGACCACATGTTCCACATTGAAGGGGCACAGAGGAAGAGCCTAGGCTGGATGTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC231332 representing NM_001199104
Red=Cloning site Green=Tags(s) MSQTSLKQKKNEPGMRSSKESLEAEKRKESDKTGVRLSNQMRRAVNPNHSLRCCPFQGHSSCRRCLCAAE GTALGPCHTIRIYIHMCLLWEQGQQITMMRGSQESSLRKTDSRGYLVRSQWSRISRSPSTKAPSIDEPRS RNTSAKLPSSSTSSRTPSTSPSLHDSSPPPLSGQPSLQPPASPQLPRSLDSRPPTPPEPDPGSRRSTKMQ ENPEAWAQGIVREIRQTRDSQPLEYSRTSPTEWKSSSQRRGIYPASTQLDRNSLSEQQQQQREDEDDYEA AYWASMRSFYEKNPSCSRPWPPKPKNAITIALSSCALFNMVDGRKIYEQEGLEKYMEYQLTNENVILTPG PAFRFVKALQYVNARLRDLYPDEQDLFDIVLMTNNHAQVGVRLINSVNHYGLLIDRFCLTGGKDPIGYLK AYLTNLYIAADSEKVQEAIQEGIASATMFDGAKDMAYCDTQLRVAFDGDAVLFSDESEHFTKEHGLDKFF QYDTLCESKPLAQGPLKGFLEDLGRLQKKFYAKNERLLCPIRTYLVTARSAASSGARVLKTLRRWGLEID EALFLAGAPKSPILVKIRPHIFFDDHMFHIEGAQRKSLGWMS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001199104 |
| ORF Size | 1806 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001199104.1, NP_001186033.1 |
| RefSeq ORF | 1809 bp |
| Locus ID | 100526794 |
| UniProt ID | Q96P26 |
| MW | 68.5 kDa |
| Gene Summary | This locus represents naturally occurring read-through transcription between the neighboring NT5C1B (5'-nucleotidase, cytosolic IB) and RDH14 (retinol dehydrogenase 14) genes on chromosome 2. Alternative splicing results in multiple transcript variants, one of which encodes a fusion protein that shares sequence identity with the products of each individual gene. [provided by RefSeq, Nov 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC231332L3 | Lenti ORF clone of Human NT5C1B-RDH14 readthrough (NT5C1B-RDH14), transcript variant 2, Myc-DDK-tagged |
CNY 7,130.00 |
|
| RC231332L4 | Lenti ORF clone of Human NT5C1B-RDH14 readthrough (NT5C1B-RDH14), transcript variant 2, mGFP tagged |
CNY 7,130.00 |
|
| RG231332 | NT5C1B (tGFP-tagged) - Human NT5C1B-RDH14 readthrough (NT5C1B-RDH14), transcript variant 2 |
CNY 5,700.00 |
