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NCF2 (NM_001190794) Human Tagged ORF Clone
CAT＃: RC231265
NCF2 (Myc-DDK-tagged)-Human neutrophil cytosolic factor 2 (NCF2), transcript variant 3
ORF Plasmid: tGFP
"NM_001190794" in other vectors (3)
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CNY 3,656.00
CNY 4,280.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NCF-2; NOXA2; P67-PHOX; P67PHOX |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC231265 representing NM_001190794
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCTGGTGGAGGCCATCAGCCTCTGGAATGAAGGGGTGCTGGCAGCGGACAAGAAGGACTGGAAGG GAGCCCTGGATGCCTTCAGTGCCGTCCAGGACCCCCACTCCCGGATTTGCTTCAACATTGGCTGCATGTA CACTATCCTGAAGAACATGACTGAAGCAGAGAAGGCCTTTACCAGAAGCATTAACCGAGACAAGCACTTG GCAGTGGCTTACTTCCAACGAGGGATGCTCTACTACCAGACAGAGAAATATGATTTGGCTATCAAAGACC TTAAAGAAGCCTTGATTCAGCTTCGAGGGAACCAGCTGATAGACTATAAGATCCTGGGGCTCCAGTTCAA GCTGTTTGCCTGTGAGAAGCAGAAGCTATATGAGCCAGTGGTGATCCCTGTGGGCAAGCTGTTTCGACCA AATGAGAGACAAGTGGCTCAGCTGGCCAAGAAGGATTACCTAGGCAAGGCGACGGTCGTGGCATCTGTGG TGGATCAAGACAGTTTCTCTGGGTTTGCCCCTCTGCAACCACAGGCAGCTGAGCCTCCACCCAGACCGAA AACCCCAGAGATCTTCAGGGCTCTGGAAGGGGAGGCTCACCGTGTGCTATTTGGGTTTGTGCCTGAGACA AAAGAAGAGCTCCAGGTCATGCCAGGGAACATTGTCTTTGTCTTGAAGAAGGGCAATGATAACTGGGCCA CGGTCATGTTCAACGGGCAGAAGGGGCTTGTTCCCTGCAACTACCTTGAACCAGTTGAGCTGCGGATCCA CCCTCAGCAGCAGCCCCAGGAGGAAAGCTCTCCGCAGTCCGACATCCCAGCTCCTCCTAGTTCCAAAGCC CCTGGAAGACCCCAGCTGTCACCAGGCCAGAAACAAAAAGAAGAGCCTAAGGAAGTGAAGCTCAGTGTTC CCATGCCCTACACACTCAAGGTGCACTACAAGTACACGGTAGTCATGAAGACTCAGCCCGGGCTCCCCTA CAGCCAGGTCCGGGACATGGTGTCTAAGAAACTGGAGCTCCGGCTGGAACACACTAAGCTGAGCTATCGG CCTCGGGACAGCAATGAGCTGGTGCCCCTTTCAGAAGACAGCATGAAGGATGCCTGGGGCCAGGTGAAAA ACTACTGCCTGACTCTGTGGTGTGAGAACACAGTGGGTGACCAAGGCTTTCCAGATGAACCCAAGGAAAG TGAAAAAGCTGATGCTAATAACCAGACAACAGAACCTCAGCTTAAGAAAGGCAGCCAAGTGGAGGCACTC TTCAGTTATGAGGCTACCCAACCAGAGGACCTGGAGTTTCAGGAAGGGGATATAATCCTGGTGTTATCAA AGGTGAATGAAGAATGGCTGGAAGGGGAGTGCAAAGGGAAGGTGGGCATTTTCCCCAAAGTTTTTGTTGA AGACTGCGCAACTACAGATTTGGAAAGCACTCGGAGAGAAGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC231265 representing NM_001190794
Red=Cloning site Green=Tags(s) MSLVEAISLWNEGVLAADKKDWKGALDAFSAVQDPHSRICFNIGCMYTILKNMTEAEKAFTRSINRDKHL AVAYFQRGMLYYQTEKYDLAIKDLKEALIQLRGNQLIDYKILGLQFKLFACEKQKLYEPVVIPVGKLFRP NERQVAQLAKKDYLGKATVVASVVDQDSFSGFAPLQPQAAEPPPRPKTPEIFRALEGEAHRVLFGFVPET KEELQVMPGNIVFVLKKGNDNWATVMFNGQKGLVPCNYLEPVELRIHPQQQPQEESSPQSDIPAPPSSKA PGRPQLSPGQKQKEEPKEVKLSVPMPYTLKVHYKYTVVMKTQPGLPYSQVRDMVSKKLELRLEHTKLSYR PRDSNELVPLSEDSMKDAWGQVKNYCLTLWCENTVGDQGFPDEPKESEKADANNQTTEPQLKKGSQVEAL FSYEATQPEDLEFQEGDIILVLSKVNEEWLEGECKGKVGIFPKVFVEDCATTDLESTRREV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001190794 |
| ORF Size | 1443 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001190794.1, NP_001177723.1 |
| RefSeq ORF | 1446 bp |
| Locus ID | 4688 |
| UniProt ID | P19878 |
| Protein Families | Druggable Genome |
| Protein Pathways | Leukocyte transendothelial migration |
| MW | 54.9 kDa |
| Gene Summary | This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC231265L3 | Lenti-ORF clone of NCF2 (Myc-DDK-tagged)-Human neutrophil cytosolic factor 2 (NCF2), transcript variant 3 |
CNY 6,180.00 |
|
| RC231265L4 | Lenti-ORF clone of NCF2 (mGFP-tagged)-Human neutrophil cytosolic factor 2 (NCF2), transcript variant 3 |
CNY 6,180.00 |
|
| RG231265 | NCF2 (tGFP-tagged) - Human neutrophil cytosolic factor 2 (NCF2), transcript variant 3 |
CNY 4,750.00 |
