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ME2 (NM_001168335) Human Tagged ORF Clone
CAT＃: RC230146
- TrueORF®
ME2 (Myc-DDK-tagged)-Human malic enzyme 2, NAD(+)-dependent, mitochondrial (ME2), nuclear gene encoding mitochondrial protein, transcript variant 2
ORF Plasmid: tGFP
"NM_001168335" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ODS1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230146 representing NM_001168335
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGTCCCGGTTAAGAGTAGTTTCCACCACTTGTACTTTGGCATGTCGACATTTGCACATAAAAGAAA AAGGCAAGCCACTTATGCTGAACCCAAGAACAAACAAGGGAATGGCATTTACTTTACAAGAACGACAAAT GCTTGGTCTTCAAGGACTTCTACCTCCCAAAATAGAGACACAAGATATTCAAGCCTTACGATTTCATAGA AACTTGAAGAAAATGACTAGCCCTTTGGAAAAATATATCTACATAATGGGAATACAAGAAAGAAATGAGA AATTGTTTTATAGAATACTGCAAGATGACATTGAGAGTTTAATGCCAATTGTATATACACCGACGGTTGG TCTTGCCTGCTCCCAGTATGGACACATCTTTAGAAGACCTAAGGGATTATTTATTTCGATCTCAGACAGA GGTCATGTTAGATCAATTGTGGATAACTGGCCAGAAAATCATGTTAAGGCTGTTGTAGTGACTGATGGAG AGAGAATTCTGGGTCTTGGAGATCTGGGTGTCTATGGAATGGGAATTCCAGTAGGAAAACTTTGTTTGTA TACAGCTTGTGCAGGAATACGGCCTGATAGATGCCTGCCAGTGTGTATTGATGTGGGAACTGATAATATC GCACTCTTAAAAGACCCATTTTACATGGGCTTGTACCAGAAACGAGATCGCACACAACAGTATGATGACC TGATTGATGAGTTTATGAAAGCTATTACTGACAGATATGGCCGGAACACACTCATTCAGTTCGAAGACTT TGGAAATCATAATGCATTCAGGTTCTTGAGAAAGTACCGAGAAAAATATTGTACTTTCAATGATGATATT CAAGGGACAGCTGCAGTAGCTCTAGCAGGTCTTCTTGCAGCACAAAAAGTTATTAGTAAACCAATCTCCG AACACAAAATCTTATTCCTTGGAGCAGGAGAGGCTGCTCTTGGAATTGCAAATCTTATAGTTATGTCTAT GGTAGAAAATGGCCTGTCAGAACAAGAGGCACAAAAGAAAATCTGGATGTTTGACAAGTATGGTTTATTA GTTAAGGGACGGAAAGCAAAAATAGATAGTTATCAGGAACCATTTACTCACTCAGCCCCAGAGAGCATAC CTGATACTTTTGAAGATGCAGTGAATATACTGAAGCCTTCAACTATAATTGGAGTTGCAGGTGCTGGCCG TCTTTTCACTCCTGATGTAATCAGAGCCATGGCCTCTATCAATGAAAGGCCTGTAATATTTGCATTAAGT AATCCTACAGCACAGGCAGAGTGCACGGCTGAAGAAGCATATACACTTACAGAGGGCAGGTGTTTGTTTG CCAGTGGCAGTCCATTTGGGCCAGTGAAACTTACAGATGGGCGAGTCTTTACACCAGGTCAAGGAAACAA TGTTTATATTTTTCCAGGTTACAGAATACCTATATGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230146 representing NM_001168335
Red=Cloning site Green=Tags(s) MLSRLRVVSTTCTLACRHLHIKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHR NLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACSQYGHIFRRPKGLFISISDR GHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTDNI ALLKDPFYMGLYQKRDRTQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYREKYCTFNDDI QGTAAVALAGLLAAQKVISKPISEHKILFLGAGEAALGIANLIVMSMVENGLSEQEAQKKIWMFDKYGLL VKGRKAKIDSYQEPFTHSAPESIPDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAMASINERPVIFALS NPTAQAECTAEEAYTLTEGRCLFASGSPFGPVKLTDGRVFTPGQGNNVYIFPGYRIPIC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001168335 |
| ORF Size | 1437 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001168335.2 |
| RefSeq ORF | 1440 bp |
| Locus ID | 4200 |
| UniProt ID | P23368 |
| Protein Pathways | Pyruvate metabolism |
| MW | 54 kDa |
| Gene Summary | This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230146L3 | Lenti-ORF clone of ME2 (Myc-DDK-tagged)-Human malic enzyme 2, NAD(+)-dependent, mitochondrial (ME2), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 5,890.00 |
|
| RC230146L4 | Lenti-ORF clone of ME2 (mGFP-tagged)-Human malic enzyme 2, NAD(+)-dependent, mitochondrial (ME2), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 5,890.00 |
|
| RG230146 | ME2 (tGFP-tagged) - Human malic enzyme 2, NAD(+)-dependent, mitochondrial (ME2), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 7,456.00 |
|
| SC328784 | ME2 (untagged)-Human malic enzyme 2 NAD(+)-dependent mitochondrial (ME2) nuclear gene encoding mitochondrial protein transcript variant 2 |
CNY 7,220.00 |
