NIPA2 (NM_001184888) Human Tagged ORF Clone

CAT#: RC229908

NIPA2 (Myc-DDK-tagged)-Human non imprinted in Prader-Willi/Angelman syndrome 2 (NIPA2), transcript variant 6

ORF Plasmid: DDK tGFP



  "NM_001184888" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-NIPA2 Antibody
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms SLC57A2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC229908 representing NM_001184888
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCAGGGGCGTGGAAAATATGACTTCTATATTGGTCTGGGATTGGCTATGAGCTCCAGCATTTTCA
TTGGAGGAAGTTTCATTTTGAAAAAAAAGGGCCTCCTTCGACTTGCCAGGAAAGGCTCTATGAGAGCAGT
GGGAGCTGGTGAGGTGGCCAACTTCGCTGCGTATGCGTTTGCACCAGCCACTCTAGTGACTCCACTAGGA
GCTCTCAGCGTGCTAGTAAGTGCCATTCTTTCTTCATACTTTCTCAATGAAAGACTTAATCTTCATGGGA
AAATTGGGTGTTTGCTAAGTATTCTAGGATCTACAGTTATGGTCATTCATGCTCCAAAGGAAGAGGAGAT
TGAGACTTTAAATGAAATGTCTCACAAGCTAGGTGATCCAGGTTTTGTGGTCTTTGCAACCCTTGTGGTC
ATTGTGGCCTTGATATTAATCTTCGTGGTGGGTCCTCGCCATGGACAGACAAACATTCTTGTGTACATAA
CAATCTGCTCTGTAATCGGCGCGTTTTCAGTCTCCTGTGTGAAGGGCCTGGGCATTGCTATCAAGGAGCT
GTTTGCAGGGAAGCCTGTGCTGCGGCATCCCCTGGCTTGGATTCTGCTGCTGAGCCTCATCGTCTGTGTG
AGCACACAGATTAATTACCTAAATAGGGCCCTGGATATATTCAACACTTCCATTGTGACTCCAATATATT
ATGTATTCTTTACAACATCAGTTTTAACTTGTTCAGCTATTCTTTTTAAGGAGTGGCAAGATATGCCTGT
TGACGATGTCATTGGTACTTTGAGTGGCTTCTTTACAATCATTGTGGGGATATTCTTGTTGCATGCCTTT
AAAGACGTCAGCTTTAGTCTAGCAAGTCTGCCTGTGTCTTTTCGAAAAGACGAGAAAGCAATGAATGGCA
ATCTCTCTAATATGTATGAAGTTCTTAATAATAATGAAGAAAGCTTAACCTGTGGAATCGAACAACACAC
TGGTGAAAATGTCTCCCGAAGAAATGGAAATCTGACAGCTTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC229908 representing NM_001184888
Red=Cloning site Green=Tags(s)

MSQGRGKYDFYIGLGLAMSSSIFIGGSFILKKKGLLRLARKGSMRAVGAGEVANFAAYAFAPATLVTPLG
ALSVLVSAILSSYFLNERLNLHGKIGCLLSILGSTVMVIHAPKEEEIETLNEMSHKLGDPGFVVFATLVV
IVALILIFVVGPRHGQTNILVYITICSVIGAFSVSCVKGLGIAIKELFAGKPVLRHPLAWILLLSLIVCV
STQINYLNRALDIFNTSIVTPIYYVFFTTSVLTCSAILFKEWQDMPVDDVIGTLSGFFTIIVGIFLLHAF
KDVSFSLASLPVSFRKDEKAMNGNLSNMYEVLNNNEESLTCGIEQHTGENVSRRNGNLTAF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001184888
ORF Size 1023 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001184888.1, NP_001171817.1
RefSeq ORF 1026 bp
Locus ID 81614
UniProt ID Q8N8Q9
Protein Families Transmembrane
MW 37.4 kDa
Gene Summary This gene encodes a possible magnesium transporter. This gene is located adjacent to the imprinted domain in the Prader-Willi syndrome deletion region of chromosome 15. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 7 and 21.[provided by RefSeq, May 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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