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FHL1 (NM_001159702) Human Tagged Lenti ORF Clone
CAT＃: RC228255L4
- LentiORF®
Lenti ORF clone of Human four and a half LIM domains 1 (FHL1), transcript variant 1, mGFP tagged
"NM_001159702" in other vectors (6)
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CNY 5,890.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | FCMSU; FHL-1; FHL1A; FHL1B; FLH1A; KYOT; RBMX1A; RBMX1B; SLIM; SLIM-1; SLIM1; SLIMMER; XMPMA |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC228255).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001159702 |
| ORF Size | 969 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001159702.1 |
| RefSeq ORF | 972 bp |
| Locus ID | 2273 |
| UniProt ID | Q13642 |
| MW | 36.1 kDa |
| Gene Summary | This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.[provided by RefSeq, Nov 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228255 | FHL1 (Myc-DDK-tagged)-Human four and a half LIM domains 1 (FHL1), transcript variant 1 |
CNY 3,600.00 |
|
| RC228255L1 | Lenti ORF clone of Human four and a half LIM domains 1 (FHL1), transcript variant 1, Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC228255L2 | Lenti ORF clone of Human four and a half LIM domains 1 (FHL1), transcript variant 1, mGFP tagged |
CNY 6,000.00 |
|
| RC228255L3 | Lenti ORF clone of Human four and a half LIM domains 1 (FHL1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RG228255 | FHL1 (tGFP-tagged) - Human four and a half LIM domains 1 (FHL1), transcript variant 1 |
CNY 4,370.00 |
|
| SC326890 | FHL1 (untagged)-Human four and a half LIM domains 1 (FHL1) transcript variant 1 |
CNY 6,270.00 |
