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RNF156 (MGRN1) (NM_001142291) Human Tagged ORF Clone
CAT＃: RC227477
MGRN1 (Myc-DDK-tagged)-Human mahogunin, ring finger 1 (MGRN1), transcript variant 4
ORF Plasmid: tGFP
"NM_001142291" in other vectors (4)
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CNY 3,656.00
CNY 4,560.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RNF156 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC227477 representing NM_001142291
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTCCATTCTCAGCCGCCGCATCGCGGGGGTGGAGGACATCGACATCCAGGCGAACTCGGCCTATC GCTACCCTCCGAAGTCCGGAAACTACTTTGCTTCGCACTTTTTCATGGGAGGAGAGAAATTCGACACCCC CCACCCTGAAGGTTACCTCTTTGGAGAGAACATGGATCTGAACTTCCTGGGCAGCCGCCCGGTCCAGTTT CCCTACGTCACTCCTGCCCCCCACGAGCCCGTGAAGACGCTGCGGAGCCTGGTGAACATCCGCAAAGACT CCCTGCGGCTGGTGAGGTACAAAGACGATGCCGACAGCCCCACCGAGGACGGCGACAAGCCCCGGGTGCT CTACAGCCTGGAGTTCACCTTCGACGCCGATGCCCGCGTGGCCATCACCATCTACTGCCAGGCATCGGAG GAGTTCCTGAACGGCAGGGCAGTATACAGCCCCAAGAGCCCCTCGCTACAGTCCGAGACCGTCCACTACA AGAGAGGGGTGAGCCAGCAGTTCTCCCTGCCCTCCTTCAAGATTGACTTCTCGGAATGGAAGGATGACGA GCTGAACTTTGACCTGGACCGGGGCGTGTTTCCAGTAGTCATCCAGGCTGTGGTGGACGAAGGAGATGTG GTGGAAGTGACTGGCCACGCCCACGTGCTCTTGGCTGCCTTTGAAAAGCACATGGACGGCAGCTTCTCTG TGAAGCCTTTAAAGCAGAAGCAAATTGTGGACCGGGTCAGCTACCTCCTGCAGGAGATCTATGGCATTGA GAACAAGAACAACCAGGAGACCAAGCCCTCGGACGACGAGAACAGCGACAACAGCAACGAGTGTGTGGTG TGCCTGTCCGACCTGCGGGACACGCTGATCCTGCCCTGCCGCCACCTGTGCCTCTGTACCTCCTGCGCCG ACACGCTGCGCTACCAGGCCAACAACTGCCCCATCTGCCGGCTGCCTTTCCGGGCCCTCCTGCAGATCCG GGCGGTGCGGAAGAAGCCAGGAGCCCTGTCCCCCGTGTCCTTCAGCCCCGTCCTGGCCCAGAGCCTGGAG CATGATGAGCACTCTAACTCTGACAGCGTCCCACCTGGCTACGAGCCCATCTCGCTGCTCGAGGCGCTCA ACGGCCTCCGGGCTGTCTCCCCGGCCATCCCCTCGGCCCCTCTTTATGAAGAAATCACCTATTCAGGCAT CTCGGACGGCCTGTCCCAGGCCAGCTGTCCCCTCGCGGCTATCGACCACATCCTGGACAGCAGCCGCCAG AAGGGCAGGCCGCAGAGCAAGGCCCCCGACAGCACCCTACGGTCCCCGTCTTCCCCCATCCACGAAGAGG ATGAGGAGAAGCTCTCCGAGGACGTGGACGCCCCTCCCCCACTGGGTGGCGCAGAGCTGGCCCTGCGGGA AAGCAGCTCCCCTGAGAGTTTCATAACAGAAGAGGTTGATGAGTCGTCGTCACCACAGCAAGGGACCCGA GCAGCTTCCATTGAGAATGTCCTGCAGGACAGCAGCCCCGAGCACTGTGGCCGAGGCCCACCTGCTGACA TCTACCTGCCAGCCCTGGGGCCCGACTCCTGCTCTGTTGGTATAGACGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227477 representing NM_001142291
Red=Cloning site Green=Tags(s) MGSILSRRIAGVEDIDIQANSAYRYPPKSGNYFASHFFMGGEKFDTPHPEGYLFGENMDLNFLGSRPVQF PYVTPAPHEPVKTLRSLVNIRKDSLRLVRYKDDADSPTEDGDKPRVLYSLEFTFDADARVAITIYCQASE EFLNGRAVYSPKSPSLQSETVHYKRGVSQQFSLPSFKIDFSEWKDDELNFDLDRGVFPVVIQAVVDEGDV VEVTGHAHVLLAAFEKHMDGSFSVKPLKQKQIVDRVSYLLQEIYGIENKNNQETKPSDDENSDNSNECVV CLSDLRDTLILPCRHLCLCTSCADTLRYQANNCPICRLPFRALLQIRAVRKKPGALSPVSFSPVLAQSLE HDEHSNSDSVPPGYEPISLLEALNGLRAVSPAIPSAPLYEEITYSGISDGLSQASCPLAAIDHILDSSRQ KGRPQSKAPDSTLRSPSSPIHEEDEEKLSEDVDAPPPLGGAELALRESSSPESFITEEVDESSSPQQGTR AASIENVLQDSSPEHCGRGPPADIYLPALGPDSCSVGIDE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001142291 |
| ORF Size | 1343 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001142291.2, NP_001135763.2 |
| RefSeq ORF | 1593 bp |
| Locus ID | 23295 |
| UniProt ID | O60291 |
| Protein Pathways | Ubiquitin mediated proteolysis |
| MW | 58.2 kDa |
| Gene Summary | Mahogunin (MGRN1) is a C3HC4 RING-containing protein with E3 ubiquitin ligase activity in vitro.[supplied by OMIM, Apr 2004] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227477L3 | Lenti-ORF clone of MGRN1 (Myc-DDK-tagged)-Human mahogunin, ring finger 1 (MGRN1), transcript variant 4 |
CNY 6,460.00 |
|
| RC227477L4 | Lenti-ORF clone of MGRN1 (mGFP-tagged)-Human mahogunin, ring finger 1 (MGRN1), transcript variant 4 |
CNY 6,460.00 |
|
| RG227477 | MGRN1 (tGFP-tagged) - Human mahogunin, ring finger 1 (MGRN1), transcript variant 4 |
CNY 5,040.00 |
|
| SC326035 | MGRN1 (untagged)-Human mahogunin, ring finger 1 (MGRN1), transcript variant 4 |
CNY 8,550.00 |
