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DCTN4 (NM_001135643) Human Tagged ORF Clone
CAT＃: RC226530
DCTN4 (Myc-DDK-tagged)-Human dynactin 4 (p62) (DCTN4), transcript variant 1
ORF Plasmid: tGFP
"NM_001135643" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DYN4; P62 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226530 representing NM_001135643
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCCTTGCTGCAGTCGGACCGGGTTCTCTATCTAGTCCAGGGAGAAAAGAAGGTTCGGGCCCCGC TCTCGCAACTCTACTTCTGCCGCTATTGTAGCGAACTGCGGTCGCTGGAATGTGTGTCTCACGAGGTGGA CTCCCATTATTGTCCCAGTTGTTTAGAAAATATGCCATCGGCTGAAGCCAAACTAAAAAAGAATAGATGT GCCAATTGTTTTGACTGTCCTGGCTGCATGCACACCCTCTCTACTCGGGCCACGAGCATCTCCACACAGC TTCCAGATGACCCAGCCAAGACCACCATGAAGAAAGCCTATTACCTGGCATGTGGATTTTGTCGCTGGAC GTCTAGAGATGTGGGCATGGCAGACAAATCTGTAGCTAGTGGCGGTTGGCAGGAACCTGAAAATCCTCAC ACACAACGGATGAACAAATTGATTGAATATTACCAGCAGCTTGCTCAGAAAGAGAAGGTTGAGCGAGATC GCAAGAAACTGGCACGACGTAGAAACTATATGCCTCTGGCTTTTTCGCAACATACTATTCATGTAGTGGA CAAATATGGTCTTGGAACCAGGCTTCAGCGACCACGAGCTGGTGCATCCATCAGTACCCTTGCCGGACTT TCCCTTAAAGAAGGAGAGGATCAGAAAGAGATAAAGATTGAGCCAGCTCAGGCTGTGGATGAAGTGGAAC CTCTACCTGAAGACTATTATACAAGACCAGTAAATTTAACAGAGGTAACAACCCTTCAGCAGCGTCTGTT ACAGCCTGACTTCCAGCCAGTCTGTGCTTCACAGCTCTATCCTCGCCACAAACATCTTCTGATCAAACGG TCCCTGCGCTGCCGTAAATGTGAACATAATTTGAGCAAGCCAGAATTTAACCCAACGTCAATCAAATTCA AAATCCAGCTGGTCGCTGTCAATTATATTCCAGAAGTGAGAATCATGTCAATTCCCAACCTTCGCTACAT GAAGGAGAGCCAGGTCCTCCTGACTCTTACAAATCCAGTTGAGAACCTCACCCATGTGACTCTCTTCGAG TGTGAGGAGGGGGACCCTGATGATATCAACAGCACTGCTAAGGTGGTGGTGCCTCCCAAAGAGCTCGTTT TAGCTGGCAAGGATGCAGCAGCAGAGTACGATGAGTTGGCAGAACCTCAAGACTTTCAGGACGATCCTGA CATTATAGCCTTCAGAAAGGCCAACAAAGTGGGTATTTTCATCAAAGTTACACCACAGCGTGAGGAGGGT GAAGTGACCGTGTGCTTCAAGATGAAGCATGATTTTAAAAACCTGGCAGCCCCCATTCGCCCCATTGAAG AAAGTGACCAGGGAACAGAAGTCATCTGGCTCACCCAGCATGTGGAACTTAGCTTGGGCCCACTTCTTCC T ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226530 representing NM_001135643
Red=Cloning site Green=Tags(s) MASLLQSDRVLYLVQGEKKVRAPLSQLYFCRYCSELRSLECVSHEVDSHYCPSCLENMPSAEAKLKKNRC ANCFDCPGCMHTLSTRATSISTQLPDDPAKTTMKKAYYLACGFCRWTSRDVGMADKSVASGGWQEPENPH TQRMNKLIEYYQQLAQKEKVERDRKKLARRRNYMPLAFSQHTIHVVDKYGLGTRLQRPRAGASISTLAGL SLKEGEDQKEIKIEPAQAVDEVEPLPEDYYTRPVNLTEVTTLQQRLLQPDFQPVCASQLYPRHKHLLIKR SLRCRKCEHNLSKPEFNPTSIKFKIQLVAVNYIPEVRIMSIPNLRYMKESQVLLTLTNPVENLTHVTLFE CEEGDPDDINSTAKVVVPPKELVLAGKDAAAEYDELAEPQDFQDDPDIIAFRKANKVGIFIKVTPQREEG EVTVCFKMKHDFKNLAAPIRPIEESDQGTEVIWLTQHVELSLGPLLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001135643 |
| ORF Size | 1401 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001135643.1, NP_001129115.1 |
| RefSeq ORF | 1404 bp |
| Locus ID | 51164 |
| UniProt ID | Q9UJW0 |
| Protein Pathways | Huntington's disease |
| MW | 53 kDa |
| Gene Summary | Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226530L1 | Lenti-ORF clone of DCTN4 (Myc-DDK-tagged)-Human dynactin 4 (p62) (DCTN4), transcript variant 1 |
CNY 5,890.00 |
|
| RC226530L2 | Lenti-ORF clone of DCTN4 (mGFP-tagged)-Human dynactin 4 (p62) (DCTN4), transcript variant 1 |
CNY 7,888.00 |
|
| RC226530L3 | Lenti-ORF clone of DCTN4 (Myc-DDK-tagged)-Human dynactin 4 (p62) (DCTN4), transcript variant 1 |
CNY 5,890.00 |
|
| RC226530L4 | Lenti-ORF clone of DCTN4 (mGFP-tagged)-Human dynactin 4 (p62) (DCTN4), transcript variant 1 |
CNY 5,890.00 |
|
| RG226530 | DCTN4 (tGFP-tagged) - Human dynactin 4 (p62) (DCTN4), transcript variant 1 |
CNY 4,370.00 |
|
| SC325966 | DCTN4 (untagged)-Human dynactin 4 (p62) (DCTN4), transcript variant 1 |
CNY 7,410.00 |
