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Ephexin 1 (NGEF) (NM_001114090) Human Tagged ORF Clone
CAT＃: RC226027
NGEF (Myc-DDK-tagged)-Human neuronal guanine nucleotide exchange factor (NGEF), transcript variant 2
ORF Plasmid: tGFP
"NM_001114090" in other vectors (6)
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CNY 6,904.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARHGEF27; EPHEXIN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226027 representing NM_001114090
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGCTGGCCGCTGCCTTCAGCGCCGCCTGCGCCGTGGACCACGACAGTTCCACCTCGGAAAGCG ACGCGCGCGACTCGGCGGCGGGACACCTGCCCGGCAGTGAGTCGTCCTCCACCCCGGGAAATGGGGCCAC GCCCGAGGAGTGGCCGGCCCTGGCCGACAGCCCCACCACGCTCACCGAGGCCCTGCGGATGATCCACCCC ATTCCCGCCGACTCCTGGAGAAACCTCATTGAACAAATAGGGCTCCTGTATCAGGAATACCGAGATAAAT CGACTCTCCAAGAAATCGAAACCAGGAGGCAACAGGATGCAGAAATAGAAGACAATACCAATGGGTCCCC GGCCAGTGAGGACACCCCGGAGGAGGAAGAAGAAGAGGAGGAGGAGGAGGAGCCGGCCAGCCCACCAGAG AGGAAGACTCTGCCCCAGATCTGCCTGCTCAGTAACCCCCACTCAAGGTTCAACCTCTGGCAGGATCTTC CCGAGATCCGGAGCAGCGGGGTGCTTGAGATCCTACAGCCCGAGGAGATTAAGCTGCAGGAGGCCATGTT CGAGCTGGTCACTTCCGAGGCGTCCTACTACAAGAGTCTGAACCTGCTCGTGTCCCACTTCATGGAGAAC GAGCGGATAAGGAAGATCCTGCACCCGTCCGAGGCGCACATCCTCTTCTCCAACGTCCTGGACGTGCTGG CTGTCAGTGAGCGGTTCCTCCTGGAGCTGGAGCACCGGATGGAGGAGAACATCGTCATCTCTGACGTGTG TGACATTGTGTACCGTTATGCGGCCGACCACTTCTCTGTCTACATCACCTACGTCAGCAATCAGACCTAC CAGGAGCGGACCTATAAGCAGCTGCTCCAGGAGAAGGCAGCTTTCCGGGAGCTGATCGCGCAGCTAGAGC TCGACCCCAAGTGCAGGGGGCTGCCCTTCTCCTCCTTCCTCATCCTGCCTTTCCAGAGGATCACACGCCT CAAGCTGTTGGTCCAGAACATCCTGAAGAGGGTAGAAGAGAGGTCTGAGCGGGAGTGCACTGCTTTGGAT GCTCACAAGGAGCTGGAAATGGTGGTGAAGGCATGCAACGAGGGCGTCAGGAAAATGAGCCGCACGGAAC AGATGATCAGCATTCAGAAGAAGATGGAGTTCAAGATCAAGTCGGTGCCCATCATCTCCCACTCCCGCTG GCTGCTGAAGCAGGGTGAGCTGCAGCAGATGTCAGGCCCCAAGACCTCCCGGACCCTGAGGACCAAGAAG CTCTTCCACGAAATTTACCTCTTCCTGTTCAACGACCTGCTGGTGATCTGCCGGCAGATTCCAGGAGACA AGTACCAGGTATTTGACTCAGCTCCGCGGGGACTGCTGCGTGTGGAGGAGCTGGAGGACCAGGGCCAGAC GCTGGCCAACGTGTTCATCCTGCGGCTGCTGGAGAACGCAGATGACCGGGAGGCCACCTACATGCTAAAG GCGTCCTCTCAGAGTGAGATGAAGCGTTGGATGACCTCACTGGCCCCCAACAGGAGGACCAAGTTTGTTT CGTTCACATCCCGGCTGCTGGACTGCCCCCAGGTCCAGTGCGTGCACCCATACGTGGCTCAGCAGCCAGA CGAGCTGACGCTGGAGCTCGCCGACATCCTCAACATCCTGGACAAGACTGACGACGGGTGGATCTTTGGC GAGCGTCTGCACGACCAGGAGAGAGGCTGGTTCCCCAGCTCCATGACTGAGGAGATCTTGAATCCCAAGA TCCGGTCCCAGAACCTCAAGGAATGTTTCCGTGTCCACAAGATGGATGACCCTCAGCGCAGCCAGAACAA GGACCGCAGGAAGCTGGGCAGCCGGAATCGGCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226027 representing NM_001114090
Red=Cloning site Green=Tags(s) MELLAAAFSAACAVDHDSSTSESDARDSAAGHLPGSESSSTPGNGATPEEWPALADSPTTLTEALRMIHP IPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIEDNTNGSPASEDTPEEEEEEEEEEEPASPPE RKTLPQICLLSNPHSRFNLWQDLPEIRSSGVLEILQPEEIKLQEAMFELVTSEASYYKSLNLLVSHFMEN ERIRKILHPSEAHILFSNVLDVLAVSERFLLELEHRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTY QERTYKQLLQEKAAFRELIAQLELDPKCRGLPFSSFLILPFQRITRLKLLVQNILKRVEERSERECTALD AHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKK LFHEIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQTLANVFILRLLENADDREATYMLK ASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILDKTDDGWIFG ERLHDQERGWFPSSMTEEILNPKIRSQNLKECFRVHKMDDPQRSQNKDRRKLGSRNRQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001114090 |
| ORF Size | 1854 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001114090.2 |
| RefSeq ORF | 1857 bp |
| Locus ID | 25791 |
| UniProt ID | Q8N5V2 |
| Protein Pathways | Axon guidance |
| MW | 71.2 kDa |
| Gene Summary | Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226027L1 | Lenti-ORF clone of NGEF (Myc-DDK-tagged)-Human neuronal guanine nucleotide exchange factor (NGEF), transcript variant 2 |
CNY 9,304.00 |
|
| RC226027L2 | Lenti-ORF clone of NGEF (mGFP-tagged)-Human neuronal guanine nucleotide exchange factor (NGEF), transcript variant 2 |
CNY 6,460.00 |
|
| RC226027L3 | Lenti-ORF clone of NGEF (Myc-DDK-tagged)-Human neuronal guanine nucleotide exchange factor (NGEF), transcript variant 2 |
CNY 6,460.00 |
|
| RC226027L4 | Lenti-ORF clone of NGEF (mGFP-tagged)-Human neuronal guanine nucleotide exchange factor (NGEF), transcript variant 2 |
CNY 6,460.00 |
|
| RG226027 | NGEF (tGFP-tagged) - Human neuronal guanine nucleotide exchange factor (NGEF), transcript variant 2 |
CNY 5,040.00 |
|
| SC318956 | NGEF (untagged)-Human neuronal guanine nucleotide exchange factor (NGEF), transcript variant 2 |
CNY 6,912.00 |
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