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PFKFB1 (NM_002625) Human Tagged ORF Clone
CAT＃: RC224599
PFKFB1 (Myc-DDK-tagged)-Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1)
ORF Plasmid: tGFP
"NM_002625" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | F6PK; HL2K; PFRX |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224599 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCCAGAGATGGGAGAGCTCACCCAAACCAGGTTGCAGAAGATCTGGATTCCACACAGCAGCGGCA GCAGCAGGCTGCAACGGAGAAGGGGCTCATCCATACCCCAGTTTACCAATTCCCCCACAATGGTGATCAT GGTGGGTTTACCAGCTCGAGGCAAGACCTATATCTCCACAAAGCTCACACGATATCTCAACTGGATAGGA ACACCAACTAAAGTGTTTAATTTAGGCCAGTATCGACGAGAGGCAGTGAGCTACAAGAACTATGAATTCT TTCTTCCAGACAACATGGAAGCCCTGCAAATCAGGAAGCAGTGCGCCCTGGCAGCCCTGAAGGATGTTCA CAACTATCTCAGCCATGAGGAAGGTCATGTTGCGGTTTTTGATGCCACCAACACTACCAGAGAACGACGG TCACTGATCCTGCAGTTTGCAAAAGAACATGGTTACAAGGTGTTTTTCATTGAGTCCATTTGTAATGACC CTGGCATAATTGCAGAAAACATCAGGCAAGTGAAACTTGGCAGCCCTGATTATATAGACTGTGACCGGGA AAAGGTTCTGGAAGACTTTCTAAAGAGAATTGAGTGCTATGAGGTCAACTACCAACCCTTGGATGAGGAA CTGGACAGCCACCTGTCCTACATCAAGATCTTCGACGTGGGCACACGCTACATGGTGAACCGAGTGCAGG ATCACATCCAGAGCCGCACAGTCTACTACCTCATGAATATCCATGTCACACCTCGCTCCATCTACCTTTG CCGACATGGCGAGAGTGAACTCAACATCAGAGGCCGCATCGGAGGTGACTCTGGCCTCTCAGTTCGCGGC AAGCAGTATGCCTATGCCCTGGCCAACTTCATTCAGTCCCAGGGCATCAGCTCCCTGAAGGTGTGGACCA GTCACATGAAGAGGACCATCCAGACAGCTGAGGCCCTGGGTGTCCCCCATGAGCAGTGGAAGGCCCTGAA TGAGATTGATGCGGGTGTCTGTGAGGAGATGACCTATGAAGAAATCCAGGAACATTACCCTGAAGAATTT GCACTGCGAGACCAAGATAAATATCGCTACCGCTATCCCAAGGGAGAGTCCTATGAGGATCTGGTTCAGC GTCTGGAGCCAGTGATAATGGAGCTAGAACGACAGGAGAATGTACTGGTGATCTGCCACCAGGCTGTCAT GCGGTGCCTCCTGGCCTATTTCCTGGATAAAAGTTCAGATGAGCTTCCATATCTCAAGTGCCCTCTGCAC ACAGTGCTCAAACTCACTCCTGTGGCTTATGGCTGCAAAGTGGAATCCATCTACCTGAATGTGGAGGCCG TGAACACACACCGGGAGAAGCCTGAGAATGTGGACATCACCCGGGAACCTGAGGAAGCCCTGGATACTGT CCCAGCCCACTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224599 protein sequence
Red=Cloning site Green=Tags(s) MSPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIG TPTKVFNLGQYRREAVSYKNYEFFLPDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERR SLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEE LDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRG KQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPHEQWKALNEIDAGVCEEMTYEEIQEHYPEEF ALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLH TVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002625 |
| ORF Size | 1413 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002625.4 |
| RefSeq Size | 1756 bp |
| RefSeq ORF | 1416 bp |
| Locus ID | 5207 |
| UniProt ID | P16118 |
| Protein Families | Druggable Genome |
| Protein Pathways | Fructose and mannose metabolism |
| MW | 54.7 kDa |
| Gene Summary | This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224599L1 | Lenti ORF clone of Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC224599L2 | Lenti ORF clone of Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), mGFP tagged |
CNY 5,890.00 |
|
| RC224599L3 | Lenti ORF clone of Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC224599L4 | Lenti ORF clone of Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), mGFP tagged |
CNY 5,890.00 |
|
| RG224599 | PFKFB1 (tGFP-tagged) - Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1) |
CNY 4,370.00 |
|
| SC303230 | PFKFB1 (untagged)-Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1) |
CNY 3,656.00 |
