GPR105 (P2RY14) (NM_001081455) Human Tagged ORF Clone

CAT#: RC224391

P2RY14 (Myc-DDK-tagged)-Human purinergic receptor P2Y, G-protein coupled, 14 (P2RY14), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_001081455" in other vectors (6)

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CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


P2RY14 rabbit polyclonal antibody
    • 25 ul

CNY 800.00
CNY 1,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms BPR105; GPR105; P2Y14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC224391 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCAATTCAACCTCCACACAGCCTCCAGATGAATCCTGCTCTCAGAACCTCCTGATCACTCAGCAGA
TCATTCCTGTGCTGTACTGTATGGTCTTCATTGCAGGAATCCTACTCAATGGAGTGTCAGGATGGATATT
CTTTTACGTGCCCAGCTCTGAGAGTTTCATCATCTATCTCAAGAACATTGTTATTGCTGACTTTGTGATG
AGCCTGACTTTTCCTTTCAAGATCCTTGGTGACTCAGGCCTTGGTCCCTGGCAGCTGAACGTGTTTGTGT
GCAGGGTCTCTGCCGTGCTCTTCTACGTCAACATGTACGTCAGCATTGTGTTCTTTGGGCTCATCAGCTT
TGACAGATATTATAAAATTGTAAAGCCTCTTTGGACTTCTTTCATCCAGTCAGTGAGTTACAGCAAACTT
CTGTCAGTGATAGTATGGATGCTCATGCTCCTCCTTGCTGTTCCAAATATTATTCTCACCAACCAGAGTG
TTAGGGAGGTTACACAAATAAAATGTATAGAACTGAAAAGTGAACTGGGACGGAAGTGGCACAAAGCATC
AAACTACATCTTCGTGGCCATCTTCTGGATTGTGTTTCTTTTGTTAATCGTTTTCTATACTGCTATCACA
AAGAAAATCTTTAAGTCCCACCTTAAGTCAAGTCGGAATTCCACTTCGGTCAAAAAGAAATCTAGCCGCA
ACATATTCAGCATCGTGTTTGTGTTTTTTGTCTGTTTTGTACCTTACCATATTGCCAGAATCCCCTACAC
AAAGAGTCAGACCGAAGCTCATTACAGCTGCCAGTCAAAAGAAATCTTGCGGTATATGAAAGAATTCACT
CTGCTACTATCTGCTGCAAATGTATGCTTGGACCCTATTATTTATTTCTTTCTATGCCAGCCGTTTAGGG
AAATCTTATGTAAGAAATTGCACATTCCATTAAAAGCTCAGAATGACCTAGACATTTCCAGAATCAAAAG
AGGAAATACAACACTTGAAAGCACAGATACTTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC224391 protein sequence
Red=Cloning site Green=Tags(s)

MINSTSTQPPDESCSQNLLITQQIIPVLYCMVFIAGILLNGVSGWIFFYVPSSESFIIYLKNIVIADFVM
SLTFPFKILGDSGLGPWQLNVFVCRVSAVLFYVNMYVSIVFFGLISFDRYYKIVKPLWTSFIQSVSYSKL
LSVIVWMLMLLLAVPNIILTNQSVREVTQIKCIELKSELGRKWHKASNYIFVAIFWIVFLLLIVFYTAIT
KKIFKSHLKSSRNSTSVKKKSSRNIFSIVFVFFVCFVPYHIARIPYTKSQTEAHYSCQSKEILRYMKEFT
LLLSAANVCLDPIIYFFLCQPFREILCKKLHIPLKAQNDLDISRIKRGNTTLESTDTL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001081455
ORF Size 1014 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001081455.1, NP_001074924.1
RefSeq Size 2694 bp
RefSeq ORF 1017 bp
Locus ID 9934
UniProt ID Q15391
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 39 kDa
Gene Summary The product of this gene belongs to the family of G-protein coupled receptors, which contains several receptor subtypes with different pharmacological selectivity for various adenosine and uridine nucleotides. This receptor is a P2Y purinergic receptor for UDP-glucose and other UDP-sugars coupled to G-proteins. It has been implicated in extending the known immune system functions of P2Y receptors by participating in the regulation of the stem cell compartment, and it may also play a role in neuroimmune function. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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