United States
Germany
Japan
United Kingdom
China
LIPF (NM_004190) Human Tagged ORF Clone
CAT＃: RC221225
LIPF (Myc-DDK-tagged)-Human lipase, gastric (LIPF), transcript variant 2
ORF Plasmid: tGFP
"NM_004190" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GL; HGL; HLAL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221225 representing NM_004190
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCTGCTTTTAACAATGGCAAGTTTGATATCTGTACTGGGGACTACACATGGTTTGTTTGGAAAAT TACATCCTGGAAGCCCTGAAGTGACTATGAACATTAGTCAGATGATTACTTATTGGGGATACCCAAATGA AGAATATGAAGTTGTGACTGAAGATGGTTATATTCTTGAAGTCAATAGAATTCCTTATGGGAAGAAAAAT TCAGGGAATACAGGCCAGAGACCTGTTGTGTTTTTGCAGCATGGTTTGCTTGCATCAGCCACAAACTGGA TTTCCAACCTGCCGAACAACAGCCTTGCCTTCATTCTGGCAGATGCTGGTTATGATGTGTGGCTGGGCAA CAGCAGAGGAAACACCTGGGCCAGAAGAAACTTGTACTATTCACCAGATTCAGTTGAATTCTGGGCTTTC AGCTTTGATGAAATGGCTAAATATGACCTTCCAGCCACAATCGACTTCATTGTAAAGAAAACTGGACAGA AGCAGCTACACTATGTTGGCCATTCCCAGGGCACCACCATTGGTTTTATTGCCTTTTCCACCAATCCCAG CCTGGCTAAAAGAATCAAAACCTTCTATGCTCTAGCTCCTGTTGCCACTGTGAAGTATACAAAAAGCCTT ATAAACAAACTTAGATTTGTTCCTCAATCCCTCTTCAAGTTTATATTTGGTGACAAAATATTCTACCCAC ACAACTTCTTTGATCAATTTCTTGCTACTGAAGTGTGCTCCCGTGAGATGCTGAATCTCCTTTGCAGCAA TGCCTTATTTATAATTTGTGGATTTGACAGTAAGAACTTTAACACGAGTCGCTTGGATGTGTATCTATCA CATAATCCAGCAGGAACTTCTGTTCAAAACATGTTCCATTGGACCCAGGCTGTTAAGTCTGGGAAATTCC AAGCTTATGACTGGGGAAGCCCAGTTCAGAATAGGATGCACTATGATCAGTCCCAACCTCCCTACTACAA TGTGACAGCCATGAATGTACCAATTGCAGTGTGGAACGGTGGCAAGGACCTGTTGGCTGACCCCCAAGAT GTTGGCCTTTTGCTTCCAAAACTCCCCAATCTTATTTACCACAAGGAGATTCCTTTTTACAATCACTTGG ACTTTATCTGGGCAATGGATGCCCCTCAAGAAGTTTACAATGACATTGTTTCTATGATATCAGAAGATAA AAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221225 representing NM_004190
Red=Cloning site Green=Tags(s) MWLLLTMASLISVLGTTHGLFGKLHPGSPEVTMNISQMITYWGYPNEEYEVVTEDGYILEVNRIPYGKKN SGNTGQRPVVFLQHGLLASATNWISNLPNNSLAFILADAGYDVWLGNSRGNTWARRNLYYSPDSVEFWAF SFDEMAKYDLPATIDFIVKKTGQKQLHYVGHSQGTTIGFIAFSTNPSLAKRIKTFYALAPVATVKYTKSL INKLRFVPQSLFKFIFGDKIFYPHNFFDQFLATEVCSREMLNLLCSNALFIICGFDSKNFNTSRLDVYLS HNPAGTSVQNMFHWTQAVKSGKFQAYDWGSPVQNRMHYDQSQPPYYNVTAMNVPIAVWNGGKDLLADPQD VGLLLPKLPNLIYHKEIPFYNHLDFIWAMDAPQEVYNDIVSMISEDKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004190 |
| ORF Size | 1194 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004190.4 |
| RefSeq Size | 1365 bp |
| RefSeq ORF | 1197 bp |
| Locus ID | 8513 |
| UniProt ID | P07098 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Glycerolipid metabolism, Metabolic pathways |
| MW | 45.24 kDa |
| Gene Summary | This gene encodes gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221225L1 | Lenti ORF clone of Human lipase, gastric (LIPF), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC221225L2 | Lenti ORF clone of Human lipase, gastric (LIPF), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC221225L3 | Lenti ORF clone of Human lipase, gastric (LIPF), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC221225L4 | Lenti ORF clone of Human lipase, gastric (LIPF), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG221225 | LIPF (tGFP-tagged) - Human lipase, gastric (LIPF), transcript variant 2 |
CNY 4,370.00 |
|
| SC303449 | LIPF (untagged)-Human lipase, gastric (LIPF), transcript variant 2 |
CNY 3,656.00 |
