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ACSM2A (NM_001010845) Human Tagged ORF Clone
CAT＃: RC220982
ACSM2A (Myc-DDK-tagged)-Human acyl-CoA synthetase medium-chain family member 2A (ACSM2A), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_001010845" in other vectors (4)
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CNY 6,440.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A-923A4.1; ACSM2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220982 representing NM_001010845
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATTGGCTGCGAAAAGTTCAGGGACTTTGCACCCTGTGGGGTACTCAGATGTCCAGCCGCACTCTCT ACATTAATAGTAGGCAACTGGTGTCCCTGCAGTGGGGCCACCAGGAAGTGCCGGCCAAGTTTAACTTTGC TAGTGATGTGTTGGATCACTGGGCTGACATGGAGAAGGCTGGCAAGCGACTCCCAAGCCCAGCCCTGTGG TGGGTGAATGGGAAGGGGAAGGAATTAATGTGGAATTTCAGAGAACTGAGTGAAAACAGCCAGCAGGCAG CCAACGTCCTCTCGGGAGCCTGTGGCCTGCAGCGTGGGGATCGTGTGGCAGTGGTGCTGCCCCGAGTGCC TGAGTGGTGGCTGGTGATCCTGGGCTGCATTCGAGCAGGTCTCATCTTTATGCCTGGAACCATCCAGATG AAATCCACTGACATACTGTATAGGTTGCAGATGTCTAAGGCCAAGGCTATTGTTGCTGGGGATGAAGTCA TCCAAGAAGTGGACACAGTGGCATCTGAATGTCCTTCTCTGAGAATTAAGCTACTGGTGTCTGAGAAAAG CTGTGATGGGTGGCTGAACTTCAAGAAACTACTAAATGAGGCATCCACCACTCATCACTGTGTGGAGACT GGAAGCCAGGAAGCATCTGCCATCTACTTCACTAGTGGGACCAGTGGTCTTCCCAAGATGGCAGAACATT CCTACTCGAGCCTGGGCCTCAAGGCCAAGATGGATGCTGGTTGGACAGGCCTGCAAGCCTCTGATATAAT GTGGACCATATCAGACACAGGTTGGATACTGAACATCTTGTGCTCACTTATGGAACCTTGGGCATTAGGA GCATGCACATTTGTTCATCTCTTGCCAAAGTTTGACCCACTGGTTATTCTAAAGACACTCTCCAGTTATC CAATCAAGAGTATGATGGGTGCCCCCATTGTTTACCGGATGTTGCTACAGCAGGATCTTTCCAGTTACAA GTTCCCCCATCTACAGAACTGCGTCACTGTAGGGGAGTCCCTTCTTCCAGAAACTCTGGAGAACTGGAGG GCCCAGACAGGACTGGACATCCGAGAATCCTATGGCCAGACAGAAACGGGATTAACTTGCATGGTTTCCA AGACAATGAAAATCAAACCAGGATACATGGGAACGGCTGCTTCCTGTTATGATGTACAGATCATAGATGA TAAGGGCAACGTCCTGCCCCCCGGCACAGAAGGAGACATTGGCATCAGGGTCAAACCCATCAGGCCTATA GGCATCTTCTCTGGCTATGTGGACAATCCCGACAAGACAGCAGCCAACATTCGAGGAGACTTTTGGCTCC TTGGAGACCGGGGAATCAAAGATGAAGATGGGTATTTCCAGTTTATGGGACGGGCAGATGATATCATTAA CTCCAGCGGGTACCGGATTGGACCCTCGGAGGTAGAGAATGCACTGATGGAGCACCCTGCTGTGGTTGAG ACGGCCGTGATCAGCAGCCCAGACCCCGTCCGAGGAGAGGTGGTGAAGGCATTTGTGGTCCTGGCCTCGC AGTTCCTGTCCCATGACCCAGAACAGCTCACCAAGGAGCTGCAGCAGCATGTGAAGTCAGTGACAGCCCC ATACAAGTACCCAAGAAAGATAGAGTTTGTCTTGAACCTGCCCAAGACTGTCACAGGGAAAATTCAACGA GCCAAGCTTCGAGACAAGGAGTGGAAGATGTCCGGAAAAGCCCGTGCGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220982 representing NM_001010845
Red=Cloning site Green=Tags(s) MHWLRKVQGLCTLWGTQMSSRTLYINSRQLVSLQWGHQEVPAKFNFASDVLDHWADMEKAGKRLPSPALW WVNGKGKELMWNFRELSENSQQAANVLSGACGLQRGDRVAVVLPRVPEWWLVILGCIRAGLIFMPGTIQM KSTDILYRLQMSKAKAIVAGDEVIQEVDTVASECPSLRIKLLVSEKSCDGWLNFKKLLNEASTTHHCVET GSQEASAIYFTSGTSGLPKMAEHSYSSLGLKAKMDAGWTGLQASDIMWTISDTGWILNILCSLMEPWALG ACTFVHLLPKFDPLVILKTLSSYPIKSMMGAPIVYRMLLQQDLSSYKFPHLQNCVTVGESLLPETLENWR AQTGLDIRESYGQTETGLTCMVSKTMKIKPGYMGTAASCYDVQIIDDKGNVLPPGTEGDIGIRVKPIRPI GIFSGYVDNPDKTAANIRGDFWLLGDRGIKDEDGYFQFMGRADDIINSSGYRIGPSEVENALMEHPAVVE TAVISSPDPVRGEVVKAFVVLASQFLSHDPEQLTKELQQHVKSVTAPYKYPRKIEFVLNLPKTVTGKIQR AKLRDKEWKMSGKARAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001010845 |
| ORF Size | 1731 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001010845.1, NP_001010845.1 |
| RefSeq Size | 2322 bp |
| RefSeq ORF | 1734 bp |
| Locus ID | 123876 |
| Protein Pathways | Butanoate metabolism, Metabolic pathways |
| MW | 64 kDa |
| Gene Summary | This gene encodes a mitochondrial acyl-coenzyme A synthetase that is specific for medium chain fatty acids. These enzymes catalyze fatty acid activation, the first step of fatty acid metabolism, through the transfer of acyl-CoA. These enzymes also participate in the glycine conjugation pathway in the detoxification of xenobiotics such as benzoate and ibuprofen. Expression levels of this gene in the kidney may be correlated with kidney function. This gene and its paralog ACSM2B (Gene ID: 348158), both present on chromosome 16, likely arose from a chromosomal duplication event. [provided by RefSeq, May 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220982L3 | Lenti ORF clone of Human acyl-CoA synthetase medium-chain family member 2A (ACSM2A), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 8,470.00 |
|
| RC220982L4 | Lenti ORF clone of Human acyl-CoA synthetase medium-chain family member 2A (ACSM2A), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 8,470.00 |
|
| RG220982 | ACSM2A (tGFP-tagged) - Human acyl-CoA synthetase medium-chain family member 2A (ACSM2A), nuclear gene encoding mitochondrial protein |
CNY 8,040.00 |
|
| SC301474 | ACSM2A (untagged)-Human acyl-CoA synthetase medium-chain family member 2A (ACSM2A), nuclear gene encoding mitochondrial protein |
CNY 9,310.00 |
