SUPT16H (NM_007192) Human Tagged ORF Clone

CAT#: RC218797

  • TrueORF®

SUPT16H (Myc-DDK-tagged)-Human suppressor of Ty 16 homolog (S. cerevisiae) (SUPT16H)

ORF Plasmid: DDK tGFP



  "NM_007192" in other vectors (4)

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CNY 10,936.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CDC68; FACTP140; SPT16; SPT16/CDC68
Vector pCMV6-AC-Myc-DDK
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NM_007192
ORF Size 3141 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_007192.2, NP_009123.1
RefSeq Size 4696 bp
RefSeq ORF 3144 bp
Locus ID 11198
UniProt ID Q9Y5B9
Domains Peptidase_M24
Protein Families Druggable Genome, Transcription Factors
MW 119.7 kDa
Gene Summary Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. [provided by RefSeq, Feb 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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