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Cyclin E2 (CCNE2) (NM_057749) Human Tagged ORF Clone
CAT＃: RC218597
CCNE2 (Myc-DDK-tagged)-Human cyclin E2 (CCNE2)
ORF Plasmid: tGFP
"NM_057749" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CYCE2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218597 representing NM_057749
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAAGACGAAGTAGCCGTTTACAAGCTAAGCAGCAGCCCCAGCCCAGCCAGACGGAATCCCCCCAAG AAGCCCAGATAATCCAGGCCAAGAAGAGGAAAACTACCCAGGATGTCAAAAAAAGAAGAGAGGAGGTCAC CAAGAAACATCAGTATGAAATTAGGAATTGTTGGCCACCTGTATTATCTGGGGGGATCAGTCCTTGCATT ATCATTGAAACACCTCACAAAGAAATAGGAACAAGTGATTTCTCCAGATTTACAAATTACAGATTTAAAA ATCTTTTTATTAATCCTTCACCTTTGCCTGATTTAAGCTGGGGATGTTCAAAAGAAGTCTGGCTAAACAT GTTAAAAAAGGAGAGCAGATATGTTCATGACAAACATTTTGAAGTTCTGCATTCTGACTTGGAACCACAG ATGAGGTCCATACTTCTAGACTGGCTTTTAGAGGTATGTGAAGTATACACACTTCATAGGGAAACATTTT ATCTTGCACAAGACTTTTTTGATAGATTTATGTTGACACAAAAGGATATAAATAAAAATATGCTTCAACT CATTGGAATTACCTCATTATTCATTGCTTCCAAACTTGAGGAAATCTATGCTCCTAAACTCCAAGAGTTT GCTTACGTCACTGATGGTGCTTGCAGTGAAGAGGATATCTTAAGGATGGAACTCATTATATTAAAGGCTT TAAAATGGGAACTTTGTCCTGTAACAATCATCTCCTGGCTAAATCTCTTTCTCCAAGTTGATGCTCTTAA AGATGCTCCTAAAGTTCTTCTACCTCAGTATTCTCAGGAAACATTCATTCAAATAGCTCAGCTTTTAGAT CTGTGTATTCTAGCCATTGATTCATTAGAGTTCCAGTACAGAATACTGACTGCTGCTGCCTTGTGCCATT TTACCTCCATTGAAGTGGTTAAGAAAGCCTCAGGTTTGGAGTGGGACAGTATTTCAGAATGTGTAGATTG GATGGTACCTTTTGTCAATGTAGTAAAAAGTACTAGTCCAGTGAAGCTGAAGACTTTTAAGAAGATTCCT ATGGAAGACAGACATAATATCCAGACACATACAAACTATTTGGCTATGCTGGAGGAAGTAAATTACATAA ACACCTTCAGAAAAGGGGGACAGTTGTCACCAGTGTGCAATGGAGGCATTATGACACCACCGAAGAGCAC TGAAAAACCACCAGGAAAACAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218597 representing NM_057749
Red=Cloning site Green=Tags(s) MSRRSSRLQAKQQPQPSQTESPQEAQIIQAKKRKTTQDVKKRREEVTKKHQYEIRNCWPPVLSGGISPCI IIETPHKEIGTSDFSRFTNYRFKNLFINPSPLPDLSWGCSKEVWLNMLKKESRYVHDKHFEVLHSDLEPQ MRSILLDWLLEVCEVYTLHRETFYLAQDFFDRFMLTQKDINKNMLQLIGITSLFIASKLEEIYAPKLQEF AYVTDGACSEEDILRMELIILKALKWELCPVTIISWLNLFLQVDALKDAPKVLLPQYSQETFIQIAQLLD LCILAIDSLEFQYRILTAAALCHFTSIEVVKKASGLEWDSISECVDWMVPFVNVVKSTSPVKLKTFKKIP MEDRHNIQTHTNYLAMLEEVNYINTFRKGGQLSPVCNGGIMTPPKSTEKPPGKH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_057749 |
| ORF Size | 1212 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_057749.2 |
| RefSeq Size | 2748 bp |
| RefSeq ORF | 1215 bp |
| Locus ID | 9134 |
| UniProt ID | O96020 |
| Domains | cyclin_C, CYCLIN, cyclin |
| Protein Families | Druggable Genome |
| Protein Pathways | Cell cycle, Oocyte meiosis, p53 signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer |
| MW | 46.6 kDa |
| Gene Summary | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218597L1 | Lenti ORF clone of Human cyclin E2 (CCNE2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218597L2 | Lenti ORF clone of Human cyclin E2 (CCNE2), mGFP tagged |
CNY 5,890.00 |
|
| RC218597L3 | Lenti ORF clone of Human cyclin E2 (CCNE2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218597L4 | Lenti ORF clone of Human cyclin E2 (CCNE2), mGFP tagged |
CNY 5,890.00 |
|
| RG218597 | CCNE2 (tGFP-tagged) - Human cyclin E2 (CCNE2) |
CNY 4,370.00 |
|
| SC120236 | CCNE2 (untagged)-Human cyclin E2 (CCNE2) |
CNY 3,656.00 |
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