COX15 (NM_004376) Human Tagged ORF Clone

CAT#: RC215190

  • TrueORF®

COX15 (Myc-DDK-tagged)-Human COX15 homolog, cytochrome c oxidase assembly protein (yeast) (COX15), nuclear gene encoding mitochondrial protein, transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_004376" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


COX15 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CEMCOX2; MC4DN6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC215190 representing NM_004376
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCGATTGCTCTTTCCGCCGTTGAGGGCCTTGAAGGGGAGGCAGTATCTGCCGCTCCTGGCTCCTA
GGGCAGCGCCTAGAGCACAGTGTGATTGCATCAGGCGCCCTTTGAGGCCAGGGCAATACAGCACCATCTC
TGAAGTAGCTTTGCAATCTGGAAGGGGTACAGTGTCCCTTCCCTCAAAGGCTGCTGAGCGGGTGGTGGGC
CGATGGCTCCTGGTCTGCAGTGGAACAGTGGCTGGAGCAGTTATTCTTGGTGGAGTAACTAGGTTGACAG
AGTCTGGCCTCTCGATGGTAGATTGGCATTTAATAAAGGAGATGAAGCCACCTACAAGCCAAGAGGAATG
GGAAGCAGAATTCCAAAGATACCAGCAATTTCCAGAATTTAAAATCTTGAATCATGATATGACACTGACA
GAATTCAAGTTCATCTGGTACATGGAGTACTCACACCGAATGTGGGGTCGCCTTGTAGGCCTTGTGTACA
TCCTGCCTGCTGCCTACTTTTGGAGAAAGGGCTGGCTCAGCCGTGGCATGAAAGGACGTGTTCTTGCCCT
CTGTGGCCTCGTCTGCTTCCAGGGTCTGTTGGGATGGTATATGGTGAAAAGTGGACTAGAAGAAAAATCA
GACTCCCATGACATCCCTCGGGTCAGTCAGTACCGCCTTGCTGCCCACCTGGGATCAGCCCTGGTTCTTT
ATTGTGCCAGCTTGTGGACCTCACTGTCACTGCTACTCCCTCCGCACAAGTTGCCTGAAACCCACCAACT
CCTACAGTTGAGACGATTTGCTCATGGAACAGCAGGTCTGGTGTTCCTTACGGCCCTCTCAGGGGCTTTT
GTGGCAGGGCTAGATGCTGGGCTTGTTTATAACTCCTTTCCCAAAATGGGAGAATCCTGGATCCCGGAGG
ACCTCTTTACCTTCTCCCCCATCCTGAGGAATGTTTTTGAGAATCCCACCATGGTGCAGTTTGATCACCG
GATTCTGGGAATCACTTCAGTCACTGCCATTACAGTGCTCTACTTCCTCTCTCGGAGAATTCCCCTTCCT
AGAAGGACCAAGATGGCAGCAGTGACTCTGCTGGCTTTGGCGTATACACAGGGCCCTGTCTTATTCAACT
TTACTTTTAAAATCAGTGATTTGGATGAAGGCATCAGAAACATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC215190 representing NM_004376
Red=Cloning site Green=Tags(s)

MQRLLFPPLRALKGRQYLPLLAPRAAPRAQCDCIRRPLRPGQYSTISEVALQSGRGTVSLPSKAAERVVG
RWLLVCSGTVAGAVILGGVTRLTESGLSMVDWHLIKEMKPPTSQEEWEAEFQRYQQFPEFKILNHDMTLT
EFKFIWYMEYSHRMWGRLVGLVYILPAAYFWRKGWLSRGMKGRVLALCGLVCFQGLLGWYMVKSGLEEKS
DSHDIPRVSQYRLAAHLGSALVLYCASLWTSLSLLLPPHKLPETHQLLQLRRFAHGTAGLVFLTALSGAF
VAGLDAGLVYNSFPKMGESWIPEDLFTFSPILRNVFENPTMVQFDHRILGITSVTAITVLYFLSRRIPLP
RRTKMAAVTLLALAYTQGPVLFNFTFKISDLDEGIRNI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004376
ORF Size 1164 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004376.7
RefSeq Size 2884 bp
RefSeq ORF 1167 bp
Locus ID 1355
UniProt ID Q7KZN9
Domains COX15-CtaA
Protein Families Transmembrane
Protein Pathways Metabolic pathways, Oxidative phosphorylation, Porphyrin and chlorophyll metabolism
MW 43.7 kDa
Gene Summary Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be essential for the biogenesis of COX formation and may function in the hydroxylation of heme O, according to the yeast mutant studies. This protein is predicted to contain 5 transmembrane domains localized in the mitochondrial inner membrane. Alternative splicing of this gene generates two transcript variants diverging in the 3' region. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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