United States
Germany
Japan
United Kingdom
China
HCCS (NM_005333) Human Tagged ORF Clone
CAT＃: RC214346
- TrueORF®
HCCS (Myc-DDK-tagged)-Human holocytochrome c synthase (HCCS), transcript variant 1
ORF Plasmid: tGFP
"NM_005333" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CCHL; LSDMCA1; MCOPS7; MLS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214346 representing NM_005333
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTTTGTCTCCATCTGCTCCTGCTGTTGCAGTTCAGGCCTCAAATGCTTCAGCGTCCCCACCTTCAG GATGCCCGATGCATGAAGGGAAAATGAAAGGCTGTCCAGTGAATACAGAGCCATCTGGCCCAACCTGTGA GAAGAAAACATACTCTGTGCCTGCCCACCAGGAACGCGCCTATGAGTACGTGGAGTGTCCCATTAGGGGC ACTGCGGCTGAGAATAAGGAGAACCTAGATCCTTCAAATCTGATGCCACCACCAAATCAAACACCAGCTC CAGATCAGCCATTTGCATTGTCTACTGTCAGAGAAGAGTCATCCATTCCGAGAGCAGATTCAGAGAAAAA GTGGGTTTACCCTTCTGAGCAGATGTTCTGGAATGCAATGTTAAAGAAAGGGTGGAAGTGGAAGGATGAG GATATCAGTCAGAAGGATATGTATAATATCATTAGAATTCACAATCAGAATAACGAGCAGGCTTGGAAGG AGATTTTGAAGTGGGAAGCCCTTCATGCTGCAGAGTGTCCTTGTGGTCCATCATTGATCCGGTTTGGAGG GAAAGCAAAAGAGTATTCACCAAGGGCACGAATTCGTTCCTGGATGGGGTATGAGTTGCCTTTTGATAGG CACGATTGGATCATAAACCGTTGCGGGACAGAAGTTAGATATGTGATTGATTATTATGATGGTGGTGAAG TCAACAAGGACTACCAGTTCACCATCCTGGACGTCCGTCCTGCCTTAGATTCACTTTCGGCAGTATGGGA CAGAATGAAAGTCGCTTGGTGGCGTTGGACCTCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214346 representing NM_005333
Red=Cloning site Green=Tags(s) MGLSPSAPAVAVQASNASASPPSGCPMHEGKMKGCPVNTEPSGPTCEKKTYSVPAHQERAYEYVECPIRG TAAENKENLDPSNLMPPPNQTPAPDQPFALSTVREESSIPRADSEKKWVYPSEQMFWNAMLKKGWKWKDE DISQKDMYNIIRIHNQNNEQAWKEILKWEALHAAECPCGPSLIRFGGKAKEYSPRARIRSWMGYELPFDR HDWIINRCGTEVRYVIDYYDGGEVNKDYQFTILDVRPALDSLSAVWDRMKVAWWRWTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005333 |
| ORF Size | 804 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005333.5 |
| RefSeq Size | 2365 bp |
| RefSeq ORF | 807 bp |
| Locus ID | 3052 |
| UniProt ID | P53701 |
| Domains | Cyto_heme_lyase |
| Protein Pathways | Porphyrin and chlorophyll metabolism |
| MW | 30.6 kDa |
| Gene Summary | The protein encoded by this gene is an enzyme that covalently links a heme group to the apoprotein of cytochrome c. Defects in this gene are a cause of microphthalmia syndromic type 7 (MCOPS7). Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214346L3 | Lenti-ORF clone of HCCS (Myc-DDK-tagged)-Human holocytochrome c synthase (HCCS), transcript variant 1 |
CNY 5,890.00 |
|
| RC214346L4 | Lenti-ORF clone of HCCS (mGFP-tagged)-Human holocytochrome c synthase (HCCS), transcript variant 1 |
CNY 5,890.00 |
|
| RG214346 | HCCS (tGFP-tagged) - Human holocytochrome c synthase (HCCS), transcript variant 1 |
CNY 4,370.00 |
|
| SC127034 | HCCS (untagged)-Human holocytochrome c synthase (HCCS), transcript variant 1 |
CNY 2,400.00 |
